Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence
Summary: Fluorescent in situ hybridization (FISH) on the RNA moiety of human telomerase (hTR) with 50-mer probes detects hTR RNA accumulated in Cajal bodies. Using both live-cell imaging and single-molecule inexpensive FISH, our published work revealed that only a fraction of hTR localizes to Cajal...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300915 |
| _version_ | 1819009619150766080 |
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| author | Emmanuelle Querido Agnel Sfeir Pascal Chartrand |
| author_facet | Emmanuelle Querido Agnel Sfeir Pascal Chartrand |
| author_sort | Emmanuelle Querido |
| collection | DOAJ |
| description | Summary: Fluorescent in situ hybridization (FISH) on the RNA moiety of human telomerase (hTR) with 50-mer probes detects hTR RNA accumulated in Cajal bodies. Using both live-cell imaging and single-molecule inexpensive FISH, our published work revealed that only a fraction of hTR localizes to Cajal bodies, with the majority of hTR molecules distributed throughout the nucleoplasm. This protocol is an application guide to the smiFISH method for the dual detection of hTR RNA and telomeres or Cajal bodies by immunofluorescence.For complete details on the use and execution of this protocol, please refer to Laprade et al. (2020). |
| first_indexed | 2024-12-21T00:59:15Z |
| format | Article |
| id | doaj.art-aadc26f97b02435498ada899af27e14d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T00:59:15Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-aadc26f97b02435498ada899af27e14d2022-12-21T19:21:14ZengElsevierSTAR Protocols2666-16672020-09-0112100104Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with ImmunofluorescenceEmmanuelle Querido0Agnel Sfeir1Pascal Chartrand2Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC H3C 3J7, Canada; Corresponding authorSkirball Institute of Biomolecular Medicine, Cell Biology Department, NYU School of Medicine, New York, NY 10016, USADepartment of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC H3C 3J7, Canada; Corresponding authorSummary: Fluorescent in situ hybridization (FISH) on the RNA moiety of human telomerase (hTR) with 50-mer probes detects hTR RNA accumulated in Cajal bodies. Using both live-cell imaging and single-molecule inexpensive FISH, our published work revealed that only a fraction of hTR localizes to Cajal bodies, with the majority of hTR molecules distributed throughout the nucleoplasm. This protocol is an application guide to the smiFISH method for the dual detection of hTR RNA and telomeres or Cajal bodies by immunofluorescence.For complete details on the use and execution of this protocol, please refer to Laprade et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720300915 |
| spellingShingle | Emmanuelle Querido Agnel Sfeir Pascal Chartrand Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence STAR Protocols |
| title | Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence |
| title_full | Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence |
| title_fullStr | Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence |
| title_full_unstemmed | Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence |
| title_short | Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence |
| title_sort | imaging of telomerase rna by single molecule inexpensive fish combined with immunofluorescence |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300915 |
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