Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells
Oxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the <i>Allium hookeri</i> extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it wa...
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/9/1919 |
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| author | Seokmuk Park Nayeon Han Jung-Min Lee Jae-Ho Lee Seunghee Bae |
| author_facet | Seokmuk Park Nayeon Han Jung-Min Lee Jae-Ho Lee Seunghee Bae |
| author_sort | Seokmuk Park |
| collection | DOAJ |
| description | Oxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the <i>Allium hookeri</i> extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it was found that a non-cytotoxic concentration of the extracts increased the viability and size of the human DPC spheroid, which was associated with the increased expression of hair-growth-related genes in cells. To determine whether or not these effects could be attributed to intracellular anti-oxidative effects, liquid chromatography-mass spectrometry alongside various biochemical analyses are conducted herein. An ingredient called alliin was identified as one of the main components. Furthermore, AHE treatment induced a significant decrease in H<sub>2</sub>O<sub>2</sub>-mediated cytotoxicities, cell death, and cellular senescence in human DPCs. Upon analyzing these results with a molecular mechanism approach, it was shown that AHE treatment increased β-Catenin and NRF2 translocation into the nucleus while inhibiting the translocation of NF-κB (p50) through p38 and PKA-mediated phosphorylations of GSK3β, an upstream regulator of those proteins. These results overall indicate the possibility that AHE can regulate GSK3β-mediated β-Catenin, NRF2, and NF-κB signaling to enhance hair-inductive properties and ultimately protect against oxidative stress-induced cellular damage in human DPCs. |
| first_indexed | 2024-03-11T04:09:22Z |
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| id | doaj.art-aadc9c5149e544c0a0fb05db2e940c95 |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:09:22Z |
| publishDate | 2023-05-01 |
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| series | Plants |
| spelling | doaj.art-aadc9c5149e544c0a0fb05db2e940c952023-11-17T23:33:29ZengMDPI AGPlants2223-77472023-05-01129191910.3390/plants12091919Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla CellsSeokmuk Park0Nayeon Han1Jung-Min Lee2Jae-Ho Lee3Seunghee Bae4Department of Cosmetics Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Cosmetics Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDerma Bio Medical Research Center, Dermato Bio, Inc., 174-1 Songdo-dong, Yeonsu-gu, Incheon 21984, Republic of KoreaDepartment of Cosmetics Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Cosmetics Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaOxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the <i>Allium hookeri</i> extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it was found that a non-cytotoxic concentration of the extracts increased the viability and size of the human DPC spheroid, which was associated with the increased expression of hair-growth-related genes in cells. To determine whether or not these effects could be attributed to intracellular anti-oxidative effects, liquid chromatography-mass spectrometry alongside various biochemical analyses are conducted herein. An ingredient called alliin was identified as one of the main components. Furthermore, AHE treatment induced a significant decrease in H<sub>2</sub>O<sub>2</sub>-mediated cytotoxicities, cell death, and cellular senescence in human DPCs. Upon analyzing these results with a molecular mechanism approach, it was shown that AHE treatment increased β-Catenin and NRF2 translocation into the nucleus while inhibiting the translocation of NF-κB (p50) through p38 and PKA-mediated phosphorylations of GSK3β, an upstream regulator of those proteins. These results overall indicate the possibility that AHE can regulate GSK3β-mediated β-Catenin, NRF2, and NF-κB signaling to enhance hair-inductive properties and ultimately protect against oxidative stress-induced cellular damage in human DPCs.https://www.mdpi.com/2223-7747/12/9/1919alopeciaanti-hair lossanti-oxidantdermal papillahair inductivity<i>Allium hookeri</i> |
| spellingShingle | Seokmuk Park Nayeon Han Jung-Min Lee Jae-Ho Lee Seunghee Bae Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells Plants alopecia anti-hair loss anti-oxidant dermal papilla hair inductivity <i>Allium hookeri</i> |
| title | Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells |
| title_full | Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells |
| title_fullStr | Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells |
| title_full_unstemmed | Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells |
| title_short | Effects of <i>Allium hookeri</i> Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells |
| title_sort | effects of i allium hookeri i extracts on hair inductive and anti oxidative properties in human dermal papilla cells |
| topic | alopecia anti-hair loss anti-oxidant dermal papilla hair inductivity <i>Allium hookeri</i> |
| url | https://www.mdpi.com/2223-7747/12/9/1919 |
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