Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications
Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/1/338 |
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| author | Shinya Yano Megumi Mori Naozumi Teramoto Makoto Iisaka Natsumi Suzuki Masanari Noto Yasuko Kaimoto Masashi Kakimoto Michio Yamada Eri Shiratsuchi Toshiaki Shimasaki Mitsuhiro Shibata |
| author_facet | Shinya Yano Megumi Mori Naozumi Teramoto Makoto Iisaka Natsumi Suzuki Masanari Noto Yasuko Kaimoto Masashi Kakimoto Michio Yamada Eri Shiratsuchi Toshiaki Shimasaki Mitsuhiro Shibata |
| author_sort | Shinya Yano |
| collection | DOAJ |
| description | Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N′-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress–strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity. |
| first_indexed | 2024-04-11T20:49:19Z |
| format | Article |
| id | doaj.art-aadf32b0afa844e6a816d13661cc65e5 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T20:49:19Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-aadf32b0afa844e6a816d13661cc65e52022-12-22T04:03:53ZengMDPI AGMarine Drugs1660-33972015-01-0113133835310.3390/md13010338md13010338Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial ApplicationsShinya Yano0Megumi Mori1Naozumi Teramoto2Makoto Iisaka3Natsumi Suzuki4Masanari Noto5Yasuko Kaimoto6Masashi Kakimoto7Michio Yamada8Eri Shiratsuchi9Toshiaki Shimasaki10Mitsuhiro Shibata11Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanResearch & Development Division, Hayashikane Sangyo Co., Ltd., 2-4-8 Yamato-machi, Shimonoseki, Yamaguchi 750-8608, JapanResearch & Development Division, Hayashikane Sangyo Co., Ltd., 2-4-8 Yamato-machi, Shimonoseki, Yamaguchi 750-8608, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanDepartment of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, JapanPhotocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N′-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress–strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity.http://www.mdpi.com/1660-3397/13/1/338elastinfish peptidemicrofibrillated cellulosehydrogelphotocrosslinkingcomposite gel |
| spellingShingle | Shinya Yano Megumi Mori Naozumi Teramoto Makoto Iisaka Natsumi Suzuki Masanari Noto Yasuko Kaimoto Masashi Kakimoto Michio Yamada Eri Shiratsuchi Toshiaki Shimasaki Mitsuhiro Shibata Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications Marine Drugs elastin fish peptide microfibrillated cellulose hydrogel photocrosslinking composite gel |
| title | Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications |
| title_full | Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications |
| title_fullStr | Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications |
| title_full_unstemmed | Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications |
| title_short | Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications |
| title_sort | preparation of photocrosslinked fish elastin polypeptide microfibrillated cellulose composite gels with elastic properties for biomaterial applications |
| topic | elastin fish peptide microfibrillated cellulose hydrogel photocrosslinking composite gel |
| url | http://www.mdpi.com/1660-3397/13/1/338 |
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