Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes
<p>A simple, fast and direct method to determine indole-3-acetic acid (IAA) was developed using a carbon paste electrode (CPE) as working electrode and square wave voltammetry (SWV) as an<strong> </strong>electrochemical method. The experimental and instrumental parameters were opt...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Mato Grosso do Sul
2019-09-01
|
| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | http://orbital.ufms.br/index.php/Chemistry/article/view/1329 |
| _version_ | 1818976950838886400 |
|---|---|
| author | Jaqueline da Silva Santos Etenaldo Felipe Santiago Gilberto José de Arruda |
| author_facet | Jaqueline da Silva Santos Etenaldo Felipe Santiago Gilberto José de Arruda |
| author_sort | Jaqueline da Silva Santos |
| collection | DOAJ |
| description | <p>A simple, fast and direct method to determine indole-3-acetic acid (IAA) was developed using a carbon paste electrode (CPE) as working electrode and square wave voltammetry (SWV) as an<strong> </strong>electrochemical method. The experimental and instrumental parameters were optimized taking into account the values that provided the best performance in terms of peak current intensity of IAA, being the best values for: pH 1.8, Pulse amplitude (mV) 25, Step potential (mV) 12.5, Frequency (Hz) 80. After this, an analytical curve was obtained in the concentration range of 1 - 12 x 10<sup>-6</sup> mol L<sup>-1</sup>. From the analysis of the curve the limits of detection and quantification were determined, obtaining the following values, 45.73 x 10<sup>-9</sup> mol L<sup>-1 </sup>and 152.44 x 10<sup>-9</sup> mol L<sup>-1</sup>, respectively. Using the methodology developed, the amount of IAA present in the <em>Pistia stratiotes</em> extracts was determined. To eliminate possible interferences the standard addition method was performed, using 100 μL of <em>P. stratiotes</em> extract, fortified with different concentrations of the analytical standard of the IAA. Was found in average of 0.13±0.01 μg/g (IAA/plant) in the extracts (<em>n</em> = 2). The present investigation shows that the complex matrix does not interfere in the proposed method, reinforcing the applicability of the electrochemical sensors in real samples.</p><p> </p><p>DOI: <a href="http://dx.doi.org/10.17807/orbital.v11i4.1329">http://dx.doi.org/10.17807/orbital.v11i4.1329</a></p><p> </p><br /> |
| first_indexed | 2024-12-20T16:20:00Z |
| format | Article |
| id | doaj.art-aae77d3a381144afaaccdc48d804fcb1 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-12-20T16:20:00Z |
| publishDate | 2019-09-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-aae77d3a381144afaaccdc48d804fcb12022-12-21T19:33:39ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282019-09-0111426827610.17807/orbital.v11i4.1329572Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotesJaqueline da Silva Santos0Etenaldo Felipe Santiago1Gilberto José de Arruda2Universidade Estadual do Mato Grosso do SulPrograma de Pós Graduação em Recursos NaturaisUniversidade Estadual do Mato Grosso do Sul<p>A simple, fast and direct method to determine indole-3-acetic acid (IAA) was developed using a carbon paste electrode (CPE) as working electrode and square wave voltammetry (SWV) as an<strong> </strong>electrochemical method. The experimental and instrumental parameters were optimized taking into account the values that provided the best performance in terms of peak current intensity of IAA, being the best values for: pH 1.8, Pulse amplitude (mV) 25, Step potential (mV) 12.5, Frequency (Hz) 80. After this, an analytical curve was obtained in the concentration range of 1 - 12 x 10<sup>-6</sup> mol L<sup>-1</sup>. From the analysis of the curve the limits of detection and quantification were determined, obtaining the following values, 45.73 x 10<sup>-9</sup> mol L<sup>-1 </sup>and 152.44 x 10<sup>-9</sup> mol L<sup>-1</sup>, respectively. Using the methodology developed, the amount of IAA present in the <em>Pistia stratiotes</em> extracts was determined. To eliminate possible interferences the standard addition method was performed, using 100 μL of <em>P. stratiotes</em> extract, fortified with different concentrations of the analytical standard of the IAA. Was found in average of 0.13±0.01 μg/g (IAA/plant) in the extracts (<em>n</em> = 2). The present investigation shows that the complex matrix does not interfere in the proposed method, reinforcing the applicability of the electrochemical sensors in real samples.</p><p> </p><p>DOI: <a href="http://dx.doi.org/10.17807/orbital.v11i4.1329">http://dx.doi.org/10.17807/orbital.v11i4.1329</a></p><p> </p><br />http://orbital.ufms.br/index.php/Chemistry/article/view/1329cyclic voltammetrysquare wave voltammetrypistia stratiotes extractcarbon paste electrode |
| spellingShingle | Jaqueline da Silva Santos Etenaldo Felipe Santiago Gilberto José de Arruda Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes Orbital: The Electronic Journal of Chemistry cyclic voltammetry square wave voltammetry pistia stratiotes extract carbon paste electrode |
| title | Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes |
| title_full | Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes |
| title_fullStr | Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes |
| title_full_unstemmed | Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes |
| title_short | Voltammetric Determination of Indole-3-acetic Acid in Extract of Pistia stratiotes |
| title_sort | voltammetric determination of indole 3 acetic acid in extract of pistia stratiotes |
| topic | cyclic voltammetry square wave voltammetry pistia stratiotes extract carbon paste electrode |
| url | http://orbital.ufms.br/index.php/Chemistry/article/view/1329 |
| work_keys_str_mv | AT jaquelinedasilvasantos voltammetricdeterminationofindole3aceticacidinextractofpistiastratiotes AT etenaldofelipesantiago voltammetricdeterminationofindole3aceticacidinextractofpistiastratiotes AT gilbertojosedearruda voltammetricdeterminationofindole3aceticacidinextractofpistiastratiotes |