Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs
<p>Abstract</p> <p>Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hypnorm and Dormicum) has an influence on measurements o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-12-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalproceduresonline.com/content/11/1/9018 |
| _version_ | 1818584471904976896 |
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| author | Gothelf Anita Hojman Pernille Gehl Julie |
| author_facet | Gothelf Anita Hojman Pernille Gehl Julie |
| author_sort | Gothelf Anita |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hypnorm and Dormicum) has an influence on measurements of hemoglobin in mice. In a cross-over study, we have compared hemoglobin levels in two groups of mice: anesthetized versus non-anesthetized and found significant decrease in hemoglobin levels in the anesthetized group (<it>p</it> < 0.05) unrelated to which group received the anesthesia. The mean hemoglobin levels after intraperitoneal administration of Hypnorm and Dormicum was 8.7 mmol/L compared to mean hemoglobin 9.9 mmol/L before anesthesia (<it>p</it> < 0.001), and the decrease lasted for more than 30 min. These results show that anesthesia can be an important confounder in studies involving measurements of hemoglobin, and this should be taken into account when planning studies and analyzing data.</p> |
| first_indexed | 2024-12-16T08:21:43Z |
| format | Article |
| id | doaj.art-aae7a83e51de491c9e31f1dde74ef7e0 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-16T08:21:43Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-aae7a83e51de491c9e31f1dde74ef7e02022-12-21T22:38:06ZengBMCBiological Procedures Online1480-92222009-12-01111901810.1007/s12575-009-9018-8Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic DrugsGothelf AnitaHojman PernilleGehl Julie<p>Abstract</p> <p>Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hypnorm and Dormicum) has an influence on measurements of hemoglobin in mice. In a cross-over study, we have compared hemoglobin levels in two groups of mice: anesthetized versus non-anesthetized and found significant decrease in hemoglobin levels in the anesthetized group (<it>p</it> < 0.05) unrelated to which group received the anesthesia. The mean hemoglobin levels after intraperitoneal administration of Hypnorm and Dormicum was 8.7 mmol/L compared to mean hemoglobin 9.9 mmol/L before anesthesia (<it>p</it> < 0.001), and the decrease lasted for more than 30 min. These results show that anesthesia can be an important confounder in studies involving measurements of hemoglobin, and this should be taken into account when planning studies and analyzing data.</p>http://www.biologicalproceduresonline.com/content/11/1/9018anesthesiahemoglobinin vivofentanylmidazolam |
| spellingShingle | Gothelf Anita Hojman Pernille Gehl Julie Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs Biological Procedures Online anesthesia hemoglobin in vivo fentanyl midazolam |
| title | Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs |
| title_full | Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs |
| title_fullStr | Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs |
| title_full_unstemmed | Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs |
| title_short | Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs |
| title_sort | change in hemoglobin levels due to anesthesia in mice an important confounder in studies on hematopoietic drugs |
| topic | anesthesia hemoglobin in vivo fentanyl midazolam |
| url | http://www.biologicalproceduresonline.com/content/11/1/9018 |
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