The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma
PurposeTo construct a prognostic signature composed of DNA repair genes to effectively predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC).MethodsAfter downloading the transcriptome and clinical data of HNSCC from the Cancer Genome Atlas (TCGA), 499 patients with HNS...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-07-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.710694/full |
| _version_ | 1818918044720693248 |
|---|---|
| author | Ruijie Ming Enhao Wang Jiahui Wei Jinxiong Shen Shimin Zong Hongjun Xiao |
| author_facet | Ruijie Ming Enhao Wang Jiahui Wei Jinxiong Shen Shimin Zong Hongjun Xiao |
| author_sort | Ruijie Ming |
| collection | DOAJ |
| description | PurposeTo construct a prognostic signature composed of DNA repair genes to effectively predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC).MethodsAfter downloading the transcriptome and clinical data of HNSCC from the Cancer Genome Atlas (TCGA), 499 patients with HNSCC were equally divided into training and testing sets. In the training set, 13 DNA repair genes were screened using univariate proportional hazard (Cox) regression analysis and least absolute shrinkage and selection operator (LASSO) Cox regression analysis to construct a risk model, which was validated in the testing set.ResultsIn the training and testing sets, there were significant differences in the clinical outcomes of patients in the high- and low-risk groups showed by Kaplan-Meier survival curves (P < 0.001). Univariate and multivariate Cox regression analyses showed that the risk score had independent prognostic predictive ability (P < 0.001). At the same time, the immune cell infiltration, immune score, immune-related gene expression, and tumor mutation burden (TMB) of patients with HNSCC were also different between the high- and low-risk groups (P < 0.05). Finally, we screened several chemotherapeutics for HNSCC, which showed significant differences in drug sensitivity between the high- and low-risk groups (P < 0.05).ConclusionThis study constructed a 13-DNA-repair-gene signature for the prognosis of HNSCC, which could accurately and independently predict the clinical outcome of the patient. We then revealed the immune landscape, TMB, and sensitivity to chemotherapy drugs in different risk groups, which might be used to guide clinical treatment decisions. |
| first_indexed | 2024-12-20T00:43:42Z |
| format | Article |
| id | doaj.art-aae91584b45d4a65adf8056dcc3b6dd6 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-20T00:43:42Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-aae91584b45d4a65adf8056dcc3b6dd62022-12-21T19:59:29ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-07-011110.3389/fonc.2021.710694710694The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell CarcinomaRuijie MingEnhao WangJiahui WeiJinxiong ShenShimin ZongHongjun XiaoPurposeTo construct a prognostic signature composed of DNA repair genes to effectively predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC).MethodsAfter downloading the transcriptome and clinical data of HNSCC from the Cancer Genome Atlas (TCGA), 499 patients with HNSCC were equally divided into training and testing sets. In the training set, 13 DNA repair genes were screened using univariate proportional hazard (Cox) regression analysis and least absolute shrinkage and selection operator (LASSO) Cox regression analysis to construct a risk model, which was validated in the testing set.ResultsIn the training and testing sets, there were significant differences in the clinical outcomes of patients in the high- and low-risk groups showed by Kaplan-Meier survival curves (P < 0.001). Univariate and multivariate Cox regression analyses showed that the risk score had independent prognostic predictive ability (P < 0.001). At the same time, the immune cell infiltration, immune score, immune-related gene expression, and tumor mutation burden (TMB) of patients with HNSCC were also different between the high- and low-risk groups (P < 0.05). Finally, we screened several chemotherapeutics for HNSCC, which showed significant differences in drug sensitivity between the high- and low-risk groups (P < 0.05).ConclusionThis study constructed a 13-DNA-repair-gene signature for the prognosis of HNSCC, which could accurately and independently predict the clinical outcome of the patient. We then revealed the immune landscape, TMB, and sensitivity to chemotherapy drugs in different risk groups, which might be used to guide clinical treatment decisions.https://www.frontiersin.org/articles/10.3389/fonc.2021.710694/fullhead and neck squamous cell carcinomaDNA repair geneprognostic signatureimmune microenvironmenttumor mutation burdendrug sensitivity |
| spellingShingle | Ruijie Ming Enhao Wang Jiahui Wei Jinxiong Shen Shimin Zong Hongjun Xiao The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma Frontiers in Oncology head and neck squamous cell carcinoma DNA repair gene prognostic signature immune microenvironment tumor mutation burden drug sensitivity |
| title | The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma |
| title_full | The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma |
| title_fullStr | The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma |
| title_short | The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma |
| title_sort | prognostic value of the dna repair gene signature in head and neck squamous cell carcinoma |
| topic | head and neck squamous cell carcinoma DNA repair gene prognostic signature immune microenvironment tumor mutation burden drug sensitivity |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.710694/full |
| work_keys_str_mv | AT ruijieming theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT enhaowang theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT jiahuiwei theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT jinxiongshen theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT shiminzong theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT hongjunxiao theprognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT ruijieming prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT enhaowang prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT jiahuiwei prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT jinxiongshen prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT shiminzong prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma AT hongjunxiao prognosticvalueofthednarepairgenesignatureinheadandnecksquamouscellcarcinoma |