Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms
It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to understand that bioelectrochemical systems (BESs) have be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1841 |
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| author | Stéphane Pinck Lucila Martínez Ostormujof Sébastien Teychené Benjamin Erable |
| author_facet | Stéphane Pinck Lucila Martínez Ostormujof Sébastien Teychené Benjamin Erable |
| author_sort | Stéphane Pinck |
| collection | DOAJ |
| description | It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to understand that bioelectrochemical systems (BESs) have been miniaturized into microBES by taking advantage of the worldwide development of microfluidics. Microfluidics tools applied to bioelectrochemistry permit even more fundamental studies of interactions and coupled phenomena occurring at the microscale, thanks, in particular, to the concomitant combination of electroanalysis, spectroscopic analytical techniques and real-time microscopy that is now possible. The analytical microsystem is therefore much better suited to the monitoring, not only of electroactive biofilm formation but also of the expression and disentangling of extracellular electron transfer (EET) catalytic mechanisms. This article reviews the details of the configurations of microfluidic BESs designed for selected objectives and their microfabrication techniques. Because the aim is to manipulate microvolumes and due to the high modularity of the experimental systems, the interfacial conditions between electrodes and electrolytes are perfectly controlled in terms of physicochemistry (pH, nutrients, chemical effectors, etc.) and hydrodynamics (shear, material transport, etc.). Most of the theoretical advances have been obtained thanks to work carried out using models of electroactive bacteria monocultures, mainly to simplify biological investigation systems. However, a huge virgin field of investigation still remains to be explored by taking advantage of the capacities of microfluidic BESs regarding the complexity and interactions of mixed electroactive biofilms. |
| first_indexed | 2024-03-10T14:39:12Z |
| format | Article |
| id | doaj.art-aae968d89ff0426e837bb85c0a8c7a5e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:39:12Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-aae968d89ff0426e837bb85c0a8c7a5e2023-11-20T21:59:00ZengMDPI AGMicroorganisms2076-26072020-11-01811184110.3390/microorganisms8111841Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial BiofilmsStéphane Pinck0Lucila Martínez Ostormujof1Sébastien Teychené2Benjamin Erable3Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, 31432 Toulouse, FranceLaboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, 31432 Toulouse, FranceLaboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, 31432 Toulouse, FranceLaboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, 31432 Toulouse, FranceIt is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to understand that bioelectrochemical systems (BESs) have been miniaturized into microBES by taking advantage of the worldwide development of microfluidics. Microfluidics tools applied to bioelectrochemistry permit even more fundamental studies of interactions and coupled phenomena occurring at the microscale, thanks, in particular, to the concomitant combination of electroanalysis, spectroscopic analytical techniques and real-time microscopy that is now possible. The analytical microsystem is therefore much better suited to the monitoring, not only of electroactive biofilm formation but also of the expression and disentangling of extracellular electron transfer (EET) catalytic mechanisms. This article reviews the details of the configurations of microfluidic BESs designed for selected objectives and their microfabrication techniques. Because the aim is to manipulate microvolumes and due to the high modularity of the experimental systems, the interfacial conditions between electrodes and electrolytes are perfectly controlled in terms of physicochemistry (pH, nutrients, chemical effectors, etc.) and hydrodynamics (shear, material transport, etc.). Most of the theoretical advances have been obtained thanks to work carried out using models of electroactive bacteria monocultures, mainly to simplify biological investigation systems. However, a huge virgin field of investigation still remains to be explored by taking advantage of the capacities of microfluidic BESs regarding the complexity and interactions of mixed electroactive biofilms.https://www.mdpi.com/2076-2607/8/11/1841microfluidicsmicrofabricationbioelectrochemical systemsmicrobial fuel cellelectroactive biofilmsextracellular electron transfer |
| spellingShingle | Stéphane Pinck Lucila Martínez Ostormujof Sébastien Teychené Benjamin Erable Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms Microorganisms microfluidics microfabrication bioelectrochemical systems microbial fuel cell electroactive biofilms extracellular electron transfer |
| title | Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms |
| title_full | Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms |
| title_fullStr | Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms |
| title_full_unstemmed | Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms |
| title_short | Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms |
| title_sort | microfluidic microbial bioelectrochemical systems an integrated investigation platform for a more fundamental understanding of electroactive bacterial biofilms |
| topic | microfluidics microfabrication bioelectrochemical systems microbial fuel cell electroactive biofilms extracellular electron transfer |
| url | https://www.mdpi.com/2076-2607/8/11/1841 |
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