Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation
This study aimed to evaluate the impact of different surface treatments (machined; sandblasted, large grit, and acid-etched (SLA); hydrophilic; and hydrophobic) on dental titanium (Ti) implant surface morphology, roughness, and biofilm formation. Four groups of Ti disks were prepared using distinct...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/6/297 |
| _version_ | 1797594080690044928 |
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| author | Bo Yun Seo KeunBaDa Son Young-Tak Son Ram Hari Dahal Shukho Kim Jungmin Kim JunHo Hwang Sung-Min Kwon Jae-Mok Lee Kyu-Bok Lee Jin-Wook Kim |
| author_facet | Bo Yun Seo KeunBaDa Son Young-Tak Son Ram Hari Dahal Shukho Kim Jungmin Kim JunHo Hwang Sung-Min Kwon Jae-Mok Lee Kyu-Bok Lee Jin-Wook Kim |
| author_sort | Bo Yun Seo |
| collection | DOAJ |
| description | This study aimed to evaluate the impact of different surface treatments (machined; sandblasted, large grit, and acid-etched (SLA); hydrophilic; and hydrophobic) on dental titanium (Ti) implant surface morphology, roughness, and biofilm formation. Four groups of Ti disks were prepared using distinct surface treatments, including femtosecond and nanosecond lasers for hydrophilic and hydrophobic treatments. Surface morphology, wettability, and roughness were assessed. Biofilm formation was evaluated by counting the colonies of <i>Aggregatibacter actinomycetemcomitans</i> (Aa), <i>Porphyromonas gingivalis</i> (Pg), and <i>Prevotella intermedia</i> (Pi) at 48 and 72 h. Statistical analysis was conducted to compare the groups using the Kruskal–Wallis H test and the Wilcoxon signed-rank test (α = 0.05). The analysis revealed that the hydrophobic group had the highest surface contact angle and roughness (<i>p</i> < 0.05), whereas the machined group had significantly higher bacterial counts across all biofilms (<i>p</i> < 0.05). At 48 h, the lowest bacterial counts were observed in the SLA group for Aa and the SLA and hydrophobic groups for Pg and Pi. At 72 h, low bacterial counts were observed in the SLA, hydrophilic, and hydrophobic groups. The results indicate that various surface treatments affect implant surface properties, with the hydrophobic surface using femtosecond laser treatment exerting a particularly inhibitory effect on initial biofilm growth (Pg and Pi). |
| first_indexed | 2024-03-11T02:18:40Z |
| format | Article |
| id | doaj.art-aae97cb5ddb64cb883a9d16fe125b663 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T02:18:40Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-aae97cb5ddb64cb883a9d16fe125b6632023-11-18T11:03:06ZengMDPI AGJournal of Functional Biomaterials2079-49832023-05-0114629710.3390/jfb14060297Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm FormationBo Yun Seo0KeunBaDa Son1Young-Tak Son2Ram Hari Dahal3Shukho Kim4Jungmin Kim5JunHo Hwang6Sung-Min Kwon7Jae-Mok Lee8Kyu-Bok Lee9Jin-Wook Kim10Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of KoreaAdvanced Dental Device Development Institute (A3DI), Kyungpook National University, Daegu 41940, Republic of KoreaAdvanced Dental Device Development Institute (A3DI), Kyungpook National University, Daegu 41940, Republic of KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of KoreaInstitute of Advanced Convergence Technology, Kyungpook National University, Daegu 41061, Republic of KoreaInstitute of Advanced Convergence Technology, Kyungpook National University, Daegu 41061, Republic of KoreaDepartment of Periodontology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of KoreaAdvanced Dental Device Development Institute (A3DI), Kyungpook National University, Daegu 41940, Republic of KoreaDepartment of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of KoreaThis study aimed to evaluate the impact of different surface treatments (machined; sandblasted, large grit, and acid-etched (SLA); hydrophilic; and hydrophobic) on dental titanium (Ti) implant surface morphology, roughness, and biofilm formation. Four groups of Ti disks were prepared using distinct surface treatments, including femtosecond and nanosecond lasers for hydrophilic and hydrophobic treatments. Surface morphology, wettability, and roughness were assessed. Biofilm formation was evaluated by counting the colonies of <i>Aggregatibacter actinomycetemcomitans</i> (Aa), <i>Porphyromonas gingivalis</i> (Pg), and <i>Prevotella intermedia</i> (Pi) at 48 and 72 h. Statistical analysis was conducted to compare the groups using the Kruskal–Wallis H test and the Wilcoxon signed-rank test (α = 0.05). The analysis revealed that the hydrophobic group had the highest surface contact angle and roughness (<i>p</i> < 0.05), whereas the machined group had significantly higher bacterial counts across all biofilms (<i>p</i> < 0.05). At 48 h, the lowest bacterial counts were observed in the SLA group for Aa and the SLA and hydrophobic groups for Pg and Pi. At 72 h, low bacterial counts were observed in the SLA, hydrophilic, and hydrophobic groups. The results indicate that various surface treatments affect implant surface properties, with the hydrophobic surface using femtosecond laser treatment exerting a particularly inhibitory effect on initial biofilm growth (Pg and Pi).https://www.mdpi.com/2079-4983/14/6/297dental implantssurface treatmentsurface roughnessbiofilm formationfemtosecond lasernanosecond laser |
| spellingShingle | Bo Yun Seo KeunBaDa Son Young-Tak Son Ram Hari Dahal Shukho Kim Jungmin Kim JunHo Hwang Sung-Min Kwon Jae-Mok Lee Kyu-Bok Lee Jin-Wook Kim Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation Journal of Functional Biomaterials dental implants surface treatment surface roughness biofilm formation femtosecond laser nanosecond laser |
| title | Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation |
| title_full | Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation |
| title_fullStr | Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation |
| title_full_unstemmed | Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation |
| title_short | Influence of Dental Titanium Implants with Different Surface Treatments Using Femtosecond and Nanosecond Lasers on Biofilm Formation |
| title_sort | influence of dental titanium implants with different surface treatments using femtosecond and nanosecond lasers on biofilm formation |
| topic | dental implants surface treatment surface roughness biofilm formation femtosecond laser nanosecond laser |
| url | https://www.mdpi.com/2079-4983/14/6/297 |
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