The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence
Abstract Background Osteosarcoma is the most common primary bone cancer occurring in young adults and the 5-year survival rate of patients with metastatic osteosarcoma is less than 30% due to high metastatic recurrence and drug resistance. Notch is a highly conserved cell to cell signaling pathway i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-03-01
|
| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-021-02372-y |
| _version_ | 1797999489784479744 |
|---|---|
| author | Jianping Zhang Na Li Siyu Lu Yanling Chen Lequn Shan Xingcheng Zhao Yongqing Xu |
| author_facet | Jianping Zhang Na Li Siyu Lu Yanling Chen Lequn Shan Xingcheng Zhao Yongqing Xu |
| author_sort | Jianping Zhang |
| collection | DOAJ |
| description | Abstract Background Osteosarcoma is the most common primary bone cancer occurring in young adults and the 5-year survival rate of patients with metastatic osteosarcoma is less than 30% due to high metastatic recurrence and drug resistance. Notch is a highly conserved cell to cell signaling pathway in evolution, and Jagged1 is an important ligand of Notch. Although some studies have found that Notch receptors and ligands including Jagged1 were highly expressed in osteosarcoma tissues and osteosarcoma cells, the role of Jagged1 in osteosarcoma progression and metastasis are still not clear. Methods Tumor tissues were collected from 68 patients and immunohistochemical staining was employed to group these patients by expression of Jagged1. Real-time quantitative PCR and Western blotting were used to detect the expression of Jagged1. We used siRNA to knockdown the expression of Jagged1 in F5M2 cells. Colony formation assay and MTT were employed to detect and analyze the proliferation of F5M2 cells with or without knockdown of Jagged1. Transwell assay were used to detect the migration and invasion of F5M2 cells. Results In this study, we found that the high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma in 68 clinical specimens. The expression of Jagged1 in F5M2 cells with high metastasis was significantly higher than that in F4 cells with low metastasis. Knockdown of Jagged1 led to lower ability of proliferation, migration, and invasion in F5M2 cells. Conclusion The high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma. Knockdown of Jagged1 significantly reduced the proliferation, migration, and invasion of osteosarcoma cells. Our results suggested that knockdown of Jagged1 may be a potentially effective treatment for metastatic osteosarcoma. |
| first_indexed | 2024-04-11T11:05:31Z |
| format | Article |
| id | doaj.art-aae9b24aaabe4da69224df2f16fdef0d |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T11:05:31Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-aae9b24aaabe4da69224df2f16fdef0d2022-12-22T04:28:22ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-03-011611810.1186/s13018-021-02372-yThe role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrenceJianping Zhang0Na Li1Siyu Lu2Yanling Chen3Lequn Shan4Xingcheng Zhao5Yongqing Xu6Department of Orthopedic Surgery, 920th Hospital of Joint Logistics Support ForceDepartment of Oncology, Tangdu Hospital, Fourth Military Medical UniversityDepartment of Orthopedic Surgery, 920th Hospital of Joint Logistics Support ForceDepartment of Orthopedic Surgery, 920th Hospital of Joint Logistics Support ForceDepartment of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical UniversitySchool of Aerospace Medicine, Fourth Military Medical UniversityDepartment of Orthopedic Surgery, 920th Hospital of Joint Logistics Support ForceAbstract Background Osteosarcoma is the most common primary bone cancer occurring in young adults and the 5-year survival rate of patients with metastatic osteosarcoma is less than 30% due to high metastatic recurrence and drug resistance. Notch is a highly conserved cell to cell signaling pathway in evolution, and Jagged1 is an important ligand of Notch. Although some studies have found that Notch receptors and ligands including Jagged1 were highly expressed in osteosarcoma tissues and osteosarcoma cells, the role of Jagged1 in osteosarcoma progression and metastasis are still not clear. Methods Tumor tissues were collected from 68 patients and immunohistochemical staining was employed to group these patients by expression of Jagged1. Real-time quantitative PCR and Western blotting were used to detect the expression of Jagged1. We used siRNA to knockdown the expression of Jagged1 in F5M2 cells. Colony formation assay and MTT were employed to detect and analyze the proliferation of F5M2 cells with or without knockdown of Jagged1. Transwell assay were used to detect the migration and invasion of F5M2 cells. Results In this study, we found that the high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma in 68 clinical specimens. The expression of Jagged1 in F5M2 cells with high metastasis was significantly higher than that in F4 cells with low metastasis. Knockdown of Jagged1 led to lower ability of proliferation, migration, and invasion in F5M2 cells. Conclusion The high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma. Knockdown of Jagged1 significantly reduced the proliferation, migration, and invasion of osteosarcoma cells. Our results suggested that knockdown of Jagged1 may be a potentially effective treatment for metastatic osteosarcoma.https://doi.org/10.1186/s13018-021-02372-yOsteosarcomaJagged1NotchMetastasisRecurrence |
| spellingShingle | Jianping Zhang Na Li Siyu Lu Yanling Chen Lequn Shan Xingcheng Zhao Yongqing Xu The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence Journal of Orthopaedic Surgery and Research Osteosarcoma Jagged1 Notch Metastasis Recurrence |
| title | The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence |
| title_full | The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence |
| title_fullStr | The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence |
| title_full_unstemmed | The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence |
| title_short | The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence |
| title_sort | role of notch ligand jagged1 in osteosarcoma proliferation metastasis and recurrence |
| topic | Osteosarcoma Jagged1 Notch Metastasis Recurrence |
| url | https://doi.org/10.1186/s13018-021-02372-y |
| work_keys_str_mv | AT jianpingzhang theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT nali theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT siyulu theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT yanlingchen theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT lequnshan theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT xingchengzhao theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT yongqingxu theroleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT jianpingzhang roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT nali roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT siyulu roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT yanlingchen roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT lequnshan roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT xingchengzhao roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence AT yongqingxu roleofnotchligandjagged1inosteosarcomaproliferationmetastasisandrecurrence |