Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies
Most vertebrates host a wide variety of haematophagous parasites, which may play an important role in the transmission of vector-borne microorganisms to hosts. Surveillance is usually performed by collecting blood and/or tissue samples from vertebrate hosts. There are multiple methods to obtain samp...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite200069/parasite200069.html |
| _version_ | 1827613891074457600 |
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| author | Szentiványi Tamara Markotter Wanda Dietrich Muriel Clément Laura Ançay Laurie Brun Loïc Genzoni Eléonore Kearney Teresa Seamark Ernest Estók Peter Christe Philippe Glaizot Olivier |
| author_facet | Szentiványi Tamara Markotter Wanda Dietrich Muriel Clément Laura Ançay Laurie Brun Loïc Genzoni Eléonore Kearney Teresa Seamark Ernest Estók Peter Christe Philippe Glaizot Olivier |
| author_sort | Szentiványi Tamara |
| collection | DOAJ |
| description | Most vertebrates host a wide variety of haematophagous parasites, which may play an important role in the transmission of vector-borne microorganisms to hosts. Surveillance is usually performed by collecting blood and/or tissue samples from vertebrate hosts. There are multiple methods to obtain samples, which can be stored for decades if properly kept. However, blood sampling is considered an invasive method and may possibly be harmful to the sampled individual. In this study, we investigated the use of ectoparasites as a tool to acquire molecular information about the presence and diversity of infectious microorganism in host populations. We tested the presence of three distinct vector-borne microorganisms in both bat blood and bat flies: Bartonella bacteria, malaria-like Polychromophilus sp. (Apicomplexa), and Trypanosoma sp. (Kinetoplastea). We detected the presence of these microorganisms both in bats and in their bat flies, with the exception of Trypanosoma sp. in South African bat flies. Additionally, we found Bartonella sp. in bat flies from one population in Spain, suggesting its presence in the host population even if not detected in bats. Bartonella and Polychromophilus infection showed the highest prevalence in both bat and bat fly populations. Single, co- and triple infections were also frequently present in both. We highlight the use of haematophagous ectoparasites to study the presence of infectious microorganism in host blood and its use as an alternative, less invasive sampling method. |
| first_indexed | 2024-03-09T08:46:05Z |
| format | Article |
| id | doaj.art-aaed87c92f0e4530bc7140ac3258c4a5 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:46:05Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-aaed87c92f0e4530bc7140ac3258c4a52023-12-02T15:27:08ZengEDP SciencesParasite1776-10422020-01-01277210.1051/parasite/2020069parasite200069Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat fliesSzentiványi Tamarahttps://orcid.org/0000-0001-8123-0374Markotter Wandahttps://orcid.org/0000-0002-7550-0080Dietrich Muriel0https://orcid.org/0000-0002-2665-7970Clément Laura1Ançay Laurie2https://orcid.org/0000-0002-4325-0291Brun Loïc3https://orcid.org/0000-0003-3009-9091Genzoni Eléonore4Kearney Teresahttps://orcid.org/0000-0002-0050-4060Seamark Ernest5https://orcid.org/0000-0001-9741-9147Estók Peter6Christe Philippe7https://orcid.org/0000-0002-8605-7002Glaizot Olivierhttps://orcid.org/0000-0001-9116-3355UMR Processus Infectieux en Milieu Insulaire TropicalDepartment of Ecology and Evolution, University of LausanneDepartment of Ecology and Evolution, University of LausanneDepartment of Ecology and Evolution, University of LausanneDepartment of Ecology and Evolution, University of LausanneAfricanBats NPCDepartment of Zoology, Eszterházy Károly UniversityDepartment of Ecology and Evolution, University of LausanneMost vertebrates host a wide variety of haematophagous parasites, which may play an important role in the transmission of vector-borne microorganisms to hosts. Surveillance is usually performed by collecting blood and/or tissue samples from vertebrate hosts. There are multiple methods to obtain samples, which can be stored for decades if properly kept. However, blood sampling is considered an invasive method and may possibly be harmful to the sampled individual. In this study, we investigated the use of ectoparasites as a tool to acquire molecular information about the presence and diversity of infectious microorganism in host populations. We tested the presence of three distinct vector-borne microorganisms in both bat blood and bat flies: Bartonella bacteria, malaria-like Polychromophilus sp. (Apicomplexa), and Trypanosoma sp. (Kinetoplastea). We detected the presence of these microorganisms both in bats and in their bat flies, with the exception of Trypanosoma sp. in South African bat flies. Additionally, we found Bartonella sp. in bat flies from one population in Spain, suggesting its presence in the host population even if not detected in bats. Bartonella and Polychromophilus infection showed the highest prevalence in both bat and bat fly populations. Single, co- and triple infections were also frequently present in both. We highlight the use of haematophagous ectoparasites to study the presence of infectious microorganism in host blood and its use as an alternative, less invasive sampling method.https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite200069/parasite200069.htmlbartonellablood-samplingnon-invasive methodnycteribiidaepolychromophilustrypanosoma |
| spellingShingle | Szentiványi Tamara Markotter Wanda Dietrich Muriel Clément Laura Ançay Laurie Brun Loïc Genzoni Eléonore Kearney Teresa Seamark Ernest Estók Peter Christe Philippe Glaizot Olivier Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies Parasite bartonella blood-sampling non-invasive method nycteribiidae polychromophilus trypanosoma |
| title | Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies |
| title_full | Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies |
| title_fullStr | Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies |
| title_full_unstemmed | Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies |
| title_short | Host conservation through their parasites: molecular surveillance of vector-borne microorganisms in bats using ectoparasitic bat flies |
| title_sort | host conservation through their parasites molecular surveillance of vector borne microorganisms in bats using ectoparasitic bat flies |
| topic | bartonella blood-sampling non-invasive method nycteribiidae polychromophilus trypanosoma |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite200069/parasite200069.html |
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