Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats
Increased blood pressure and plasma cholesterol concentration are the major risk factors for cardiovascular diseases. We have previously shown that fermented milk products containing casein-derived bioactive tripeptides, isoleucine-proline-proline (Ile-Pro-Pro) and valine-proline-proline (Val-Pro-Pr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009-10-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464609000590 |
| _version_ | 1818417880870420480 |
|---|---|
| author | Pauliina Jäkälä Anne Hakala Anu M. Turpeinen Riitta Korpela Heikki Vapaatalo |
| author_facet | Pauliina Jäkälä Anne Hakala Anu M. Turpeinen Riitta Korpela Heikki Vapaatalo |
| author_sort | Pauliina Jäkälä |
| collection | DOAJ |
| description | Increased blood pressure and plasma cholesterol concentration are the major risk factors for cardiovascular diseases. We have previously shown that fermented milk products containing casein-derived bioactive tripeptides, isoleucine-proline-proline (Ile-Pro-Pro) and valine-proline-proline (Val-Pro-Pro) partly inhibit the blood pressure increase in spontaneously hypertensive rats (SHR). In the present study, we investigated the effect of bioactive tripeptide-containing fermented milk products (with or without plant sterols) on blood pressure and vascular function in salt-loaded type 2 diabetic Goto–Kakizaki (GK) rats after 8 weeks’ treatment. The development of blood pressure was attenuated in the groups receiving tripeptide-containing products (−10 to −12 mmHg vs. the control product group, P < 0.01). Tripeptide-containing products enhanced the impaired endothelium-dependent relaxation of mesenteric arteries. Also the role of endothelium-derived hyperpolarizing factor (EDHF) was demonstrated. Interestingly, pretreatment of arterial rings with verapamil normalized the impaired endothelial function. Endothelium-independent relaxations were similar between the groups. Furthermore, tripeptides had positive effects on renin–angiotensin–aldosterone system. |
| first_indexed | 2024-12-14T12:13:49Z |
| format | Article |
| id | doaj.art-aaef256b8abf4a62a5c85c68403e401a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T12:13:49Z |
| publishDate | 2009-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-aaef256b8abf4a62a5c85c68403e401a2022-12-21T23:01:41ZengElsevierJournal of Functional Foods1756-46462009-10-0114366374Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki ratsPauliina Jäkälä0Anne Hakala1Anu M. Turpeinen2Riitta Korpela3Heikki Vapaatalo4Institute of Biomedicine, Pharmacology, University of Helsinki, P.O. Box 63, 00014 University of Helsinki, Finland; Corresponding author: Tel.: +358 191 25345; fax: +358 191 25364.Institute of Biomedicine, Pharmacology, University of Helsinki, P.O. Box 63, 00014 University of Helsinki, FinlandValio Ltd., R&D, P.O. Box 30, 00039 Valio, FinlandInstitute of Biomedicine, Pharmacology, University of Helsinki, P.O. Box 63, 00014 University of Helsinki, Finland; Valio Ltd., R&D, P.O. Box 30, 00039 Valio, FinlandInstitute of Biomedicine, Pharmacology, University of Helsinki, P.O. Box 63, 00014 University of Helsinki, FinlandIncreased blood pressure and plasma cholesterol concentration are the major risk factors for cardiovascular diseases. We have previously shown that fermented milk products containing casein-derived bioactive tripeptides, isoleucine-proline-proline (Ile-Pro-Pro) and valine-proline-proline (Val-Pro-Pro) partly inhibit the blood pressure increase in spontaneously hypertensive rats (SHR). In the present study, we investigated the effect of bioactive tripeptide-containing fermented milk products (with or without plant sterols) on blood pressure and vascular function in salt-loaded type 2 diabetic Goto–Kakizaki (GK) rats after 8 weeks’ treatment. The development of blood pressure was attenuated in the groups receiving tripeptide-containing products (−10 to −12 mmHg vs. the control product group, P < 0.01). Tripeptide-containing products enhanced the impaired endothelium-dependent relaxation of mesenteric arteries. Also the role of endothelium-derived hyperpolarizing factor (EDHF) was demonstrated. Interestingly, pretreatment of arterial rings with verapamil normalized the impaired endothelial function. Endothelium-independent relaxations were similar between the groups. Furthermore, tripeptides had positive effects on renin–angiotensin–aldosterone system.http://www.sciencedirect.com/science/article/pii/S1756464609000590HypertensionType 2 diabetesGoto–Kakizaki ratBioactive peptidesVascular functionEndothelium |
| spellingShingle | Pauliina Jäkälä Anne Hakala Anu M. Turpeinen Riitta Korpela Heikki Vapaatalo Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats Journal of Functional Foods Hypertension Type 2 diabetes Goto–Kakizaki rat Bioactive peptides Vascular function Endothelium |
| title | Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats |
| title_full | Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats |
| title_fullStr | Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats |
| title_full_unstemmed | Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats |
| title_short | Casein-derived bioactive tripeptides Ile-Pro-Pro and Val-Pro-Pro attenuate the development of hypertension and improve endothelial function in salt-loaded Goto–Kakizaki rats |
| title_sort | casein derived bioactive tripeptides ile pro pro and val pro pro attenuate the development of hypertension and improve endothelial function in salt loaded goto kakizaki rats |
| topic | Hypertension Type 2 diabetes Goto–Kakizaki rat Bioactive peptides Vascular function Endothelium |
| url | http://www.sciencedirect.com/science/article/pii/S1756464609000590 |
| work_keys_str_mv | AT pauliinajakala caseinderivedbioactivetripeptidesileproproandvalproproattenuatethedevelopmentofhypertensionandimproveendothelialfunctioninsaltloadedgotokakizakirats AT annehakala caseinderivedbioactivetripeptidesileproproandvalproproattenuatethedevelopmentofhypertensionandimproveendothelialfunctioninsaltloadedgotokakizakirats AT anumturpeinen caseinderivedbioactivetripeptidesileproproandvalproproattenuatethedevelopmentofhypertensionandimproveendothelialfunctioninsaltloadedgotokakizakirats AT riittakorpela caseinderivedbioactivetripeptidesileproproandvalproproattenuatethedevelopmentofhypertensionandimproveendothelialfunctioninsaltloadedgotokakizakirats AT heikkivapaatalo caseinderivedbioactivetripeptidesileproproandvalproproattenuatethedevelopmentofhypertensionandimproveendothelialfunctioninsaltloadedgotokakizakirats |