Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology
It is known that NO is a ubiquitous mediator which acts as a universal modulator of various functions in organism and is produced by three isoforms of NO synthase. Nowadays the role of NO in the development of autoimmune diseases is actively studied. However, it remains unclear the biochemical and b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Poznan University of Medical Sciences
2015-03-01
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| Series: | Journal of Medical Science |
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| Online Access: | https://jms.ump.edu.pl/index.php/JMS/article/view/35 |
| _version_ | 1818910332100280320 |
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| author | Ulyana Efremova Nataliya Lychkovska Roman Fafula Zinoviy Vorobets |
| author_facet | Ulyana Efremova Nataliya Lychkovska Roman Fafula Zinoviy Vorobets |
| author_sort | Ulyana Efremova |
| collection | DOAJ |
| description | It is known that NO is a ubiquitous mediator which acts as a universal modulator of various functions in organism and is produced by three isoforms of NO synthase. Nowadays the role of NO in the development of autoimmune diseases is actively studied. However, it remains unclear the biochemical and biophysical mechanisms of disturbances of NOS activity in blood lymphocytes at autoimmune process. The aim of present work is to study the kinetic properties of NO-synthase of peripheral blood lymphocytes of patients with rheumatic pathology. The study was carried out on peripheral blood lymphocytes isolated from patients with rheumatoid arthritis and ankylosing spondylitis. NOS activity was determined on the saponin-permeabilized blood lymphocytes. The difference between the values of NADPH oxidation with L-Arg and with inhibitor L-NAME reflects the value of the NADPH oxidation, ie total NOS activity. The kinetic properties of NO-synthase in peripheral blood lymphocytes of patients with rheumatic pathology were studied. It was found that the development of rheumatic pathology is associated with an imbalance in the NO synthesis and changes of kinetic parameters of NOS. It was shown that reduction in eNOS activity is accompanied by a sharp increase in activity of its inducible form. It was established that inhibition of eNOS occurs by noncompetitive type. NO production in lymphocytes of patients with rheumatic diseases is mainly realized by iNOS, whereas under normal physiological conditions endothelial form of the enzyme is being involved. |
| first_indexed | 2024-12-19T22:41:07Z |
| format | Article |
| id | doaj.art-aaef3b712d164a078641793f7a4b850f |
| institution | Directory Open Access Journal |
| issn | 2353-9798 2353-9801 |
| language | English |
| last_indexed | 2024-12-19T22:41:07Z |
| publishDate | 2015-03-01 |
| publisher | Poznan University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Science |
| spelling | doaj.art-aaef3b712d164a078641793f7a4b850f2022-12-21T20:03:04ZengPoznan University of Medical SciencesJournal of Medical Science2353-97982353-98012015-03-0184110.20883/medical.e35Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathologyUlyana Efremova0Nataliya Lychkovska1Roman Fafula2Zinoviy Vorobets3Danylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineIt is known that NO is a ubiquitous mediator which acts as a universal modulator of various functions in organism and is produced by three isoforms of NO synthase. Nowadays the role of NO in the development of autoimmune diseases is actively studied. However, it remains unclear the biochemical and biophysical mechanisms of disturbances of NOS activity in blood lymphocytes at autoimmune process. The aim of present work is to study the kinetic properties of NO-synthase of peripheral blood lymphocytes of patients with rheumatic pathology. The study was carried out on peripheral blood lymphocytes isolated from patients with rheumatoid arthritis and ankylosing spondylitis. NOS activity was determined on the saponin-permeabilized blood lymphocytes. The difference between the values of NADPH oxidation with L-Arg and with inhibitor L-NAME reflects the value of the NADPH oxidation, ie total NOS activity. The kinetic properties of NO-synthase in peripheral blood lymphocytes of patients with rheumatic pathology were studied. It was found that the development of rheumatic pathology is associated with an imbalance in the NO synthesis and changes of kinetic parameters of NOS. It was shown that reduction in eNOS activity is accompanied by a sharp increase in activity of its inducible form. It was established that inhibition of eNOS occurs by noncompetitive type. NO production in lymphocytes of patients with rheumatic diseases is mainly realized by iNOS, whereas under normal physiological conditions endothelial form of the enzyme is being involved.https://jms.ump.edu.pl/index.php/JMS/article/view/35NO-synthasenitric oxidelymphocytesrheumatoid arthritisankylosing spondylitis |
| spellingShingle | Ulyana Efremova Nataliya Lychkovska Roman Fafula Zinoviy Vorobets Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology Journal of Medical Science NO-synthase nitric oxide lymphocytes rheumatoid arthritis ankylosing spondylitis |
| title | Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| title_full | Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| title_fullStr | Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| title_full_unstemmed | Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| title_short | Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| title_sort | characteristic of no synthase of peripheral blood lymphocytes of patients with rheumatic pathology |
| topic | NO-synthase nitric oxide lymphocytes rheumatoid arthritis ankylosing spondylitis |
| url | https://jms.ump.edu.pl/index.php/JMS/article/view/35 |
| work_keys_str_mv | AT ulyanaefremova characteristicofnosynthaseofperipheralbloodlymphocytesofpatientswithrheumaticpathology AT nataliyalychkovska characteristicofnosynthaseofperipheralbloodlymphocytesofpatientswithrheumaticpathology AT romanfafula characteristicofnosynthaseofperipheralbloodlymphocytesofpatientswithrheumaticpathology AT zinoviyvorobets characteristicofnosynthaseofperipheralbloodlymphocytesofpatientswithrheumaticpathology |