Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma
Weiwei Li,*,1,2 Luman Wang*,3, Chunying Shen1,2, Tingting Xu1,2, Yiwei Chu,3 Chaosu Hu1,21Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People’s Republic of China; 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People&a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-07-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/radiation-therapy-induced-reactive-oxygen-species-specifically-elimina-peer-reviewed-article-CMAR |
| _version_ | 1818886378904092672 |
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| author | Li W Wang L Shen C Xu T Chu Y Hu C |
| author_facet | Li W Wang L Shen C Xu T Chu Y Hu C |
| author_sort | Li W |
| collection | DOAJ |
| description | Weiwei Li,*,1,2 Luman Wang*,3, Chunying Shen1,2, Tingting Xu1,2, Yiwei Chu,3 Chaosu Hu1,21Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People’s Republic of China; 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China; 3Department of Immunology, School of Basic Medical Sciences, and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, People’s Republic of China*These authors contributed equally to this workPurpose: Nasopharyngeal carcinoma (NPC) is one of the most common head and neck cancers and is thought to be related to the mucosal immune system. Radiation therapy (RT) is the primary treatment for NPC due to the high radiosensitivity of cancer cells. However, little is known about the impact of RT on the mucosal immune system.Patients and methods: In this study, the expression of immune markers CD19, CD24, CD27, CD8, and IgA before and after RT, were analyzed using flow cytometry. Cytokines were assessed using the enzyme-linked immunosorbent assay. Reactive oxygen species (ROS) was assayed by flow cytometry and fluorescence staining using 2ʹ,7ʹ -dichlorofluorescein diacetate.Results: We found that primary NPC patients had a significant increase in CD19+CD138−IgA+, B cells, which was then decreased after RT. Interestingly, the changes in CD19+CD138−IgA+, B cell frequency was accompanied by corresponding frequency changes in cytotoxic T cells (CTL), which are powerful anti-tumor lymphocytes. Mechanistically, we found that ROS release during RT specifically eliminated CD19+CD138−IgA+ B cells.Conclusion: These findings suggest that RT may regulate the immune system and opens up new avenues for the utilization of immune-radiotherapy in NPC.Keywords: B cells, IgA, nasopharyngeal carcinoma, radiation therapy, regulatory immune cells |
| first_indexed | 2024-12-19T16:20:24Z |
| format | Article |
| id | doaj.art-aaf559f7888c40f19d7c018b0960d292 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-19T16:20:24Z |
| publishDate | 2019-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-aaf559f7888c40f19d7c018b0960d2922022-12-21T20:14:29ZengDove Medical PressCancer Management and Research1179-13222019-07-01Volume 116299630946923Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinomaLi WWang LShen CXu TChu YHu CWeiwei Li,*,1,2 Luman Wang*,3, Chunying Shen1,2, Tingting Xu1,2, Yiwei Chu,3 Chaosu Hu1,21Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People’s Republic of China; 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of China; 3Department of Immunology, School of Basic Medical Sciences, and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, People’s Republic of China*These authors contributed equally to this workPurpose: Nasopharyngeal carcinoma (NPC) is one of the most common head and neck cancers and is thought to be related to the mucosal immune system. Radiation therapy (RT) is the primary treatment for NPC due to the high radiosensitivity of cancer cells. However, little is known about the impact of RT on the mucosal immune system.Patients and methods: In this study, the expression of immune markers CD19, CD24, CD27, CD8, and IgA before and after RT, were analyzed using flow cytometry. Cytokines were assessed using the enzyme-linked immunosorbent assay. Reactive oxygen species (ROS) was assayed by flow cytometry and fluorescence staining using 2ʹ,7ʹ -dichlorofluorescein diacetate.Results: We found that primary NPC patients had a significant increase in CD19+CD138−IgA+, B cells, which was then decreased after RT. Interestingly, the changes in CD19+CD138−IgA+, B cell frequency was accompanied by corresponding frequency changes in cytotoxic T cells (CTL), which are powerful anti-tumor lymphocytes. Mechanistically, we found that ROS release during RT specifically eliminated CD19+CD138−IgA+ B cells.Conclusion: These findings suggest that RT may regulate the immune system and opens up new avenues for the utilization of immune-radiotherapy in NPC.Keywords: B cells, IgA, nasopharyngeal carcinoma, radiation therapy, regulatory immune cellshttps://www.dovepress.com/radiation-therapy-induced-reactive-oxygen-species-specifically-elimina-peer-reviewed-article-CMARB cellsIgANasopharyngeal carcinomaRadiation therapyRegulatory immune cells |
| spellingShingle | Li W Wang L Shen C Xu T Chu Y Hu C Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma Cancer Management and Research B cells IgA Nasopharyngeal carcinoma Radiation therapy Regulatory immune cells |
| title | Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma |
| title_full | Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma |
| title_fullStr | Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma |
| title_full_unstemmed | Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma |
| title_short | Radiation therapy-induced reactive oxygen species specifically eliminates CD19+ IgA+ B cells in nasopharyngeal carcinoma |
| title_sort | radiation therapy induced reactive oxygen species specifically eliminates cd19 nbsp iga nbsp b cells nbsp in nasopharyngeal carcinoma |
| topic | B cells IgA Nasopharyngeal carcinoma Radiation therapy Regulatory immune cells |
| url | https://www.dovepress.com/radiation-therapy-induced-reactive-oxygen-species-specifically-elimina-peer-reviewed-article-CMAR |
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