The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri
Shigella flexneri is a waterborne and foodborne pathogen that can damage human health. The exopolysaccharides (S-EPS) produced by S. flexneri CMCC51574 were found to promote biofilm formation and virulence. In this research, the crude S-EPS produced by S. flexneri CMCC51574 were separated into three...
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.807397/full |
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| author | Yinglong Song Fenglian Ma Mengying Sun Guangqing Mu Guangqing Mu Yanfeng Tuo |
| author_facet | Yinglong Song Fenglian Ma Mengying Sun Guangqing Mu Guangqing Mu Yanfeng Tuo |
| author_sort | Yinglong Song |
| collection | DOAJ |
| description | Shigella flexneri is a waterborne and foodborne pathogen that can damage human health. The exopolysaccharides (S-EPS) produced by S. flexneri CMCC51574 were found to promote biofilm formation and virulence. In this research, the crude S-EPS produced by S. flexneri CMCC51574 were separated into three main different fractions, S-EPS 1-1, S-EPS 2-1, and S-EPS 3-1. The structure of the S-ESP 2-1 was identified by FT-IR, ion chromatography analysis, methylation analysis, and NMR analysis. The main chain of S-EPS 2-1 was α-Manp-(1 → 3)-α-Manp-[(1 → 2,6)-α-Manp]15-[(1 → 2)-Manf-(1→]8; there were two branched-chain R1 and R2 with a ratio of 4:1, R1: α-Manp-(1 → 6)- and R2: α-Manp-(1 → 6)- Glc-(1 → 6)- were linked with (1 → 2,6)-α-Manp. It was found that S-EPS 2-1 exhibited the highest promoting effect on biofilm formation of S. flexneri. The S-EPS 2-1 was identified to interact with extracellular DNA (eDNA) of S. flexneri, indicating that the S-EPS 2-1 was the specific polysaccharide in the spatial structure of biofilm formation. Our research found the important role of S-EPS in S. flexneri biofilm formation, which will help us to understand the underlining mechanisms of the biofilm formation and find effective ways to prevent S. flexneri biofilm infection. |
| first_indexed | 2024-04-11T14:41:57Z |
| format | Article |
| id | doaj.art-aaf945a471c14cfda6a274dc304d3d72 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T14:41:57Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aaf945a471c14cfda6a274dc304d3d722022-12-22T04:17:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011210.3389/fmicb.2021.807397807397The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneriYinglong Song0Fenglian Ma1Mengying Sun2Guangqing Mu3Guangqing Mu4Yanfeng Tuo5School of Food Science and Technology, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian, ChinaDalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian, ChinaShigella flexneri is a waterborne and foodborne pathogen that can damage human health. The exopolysaccharides (S-EPS) produced by S. flexneri CMCC51574 were found to promote biofilm formation and virulence. In this research, the crude S-EPS produced by S. flexneri CMCC51574 were separated into three main different fractions, S-EPS 1-1, S-EPS 2-1, and S-EPS 3-1. The structure of the S-ESP 2-1 was identified by FT-IR, ion chromatography analysis, methylation analysis, and NMR analysis. The main chain of S-EPS 2-1 was α-Manp-(1 → 3)-α-Manp-[(1 → 2,6)-α-Manp]15-[(1 → 2)-Manf-(1→]8; there were two branched-chain R1 and R2 with a ratio of 4:1, R1: α-Manp-(1 → 6)- and R2: α-Manp-(1 → 6)- Glc-(1 → 6)- were linked with (1 → 2,6)-α-Manp. It was found that S-EPS 2-1 exhibited the highest promoting effect on biofilm formation of S. flexneri. The S-EPS 2-1 was identified to interact with extracellular DNA (eDNA) of S. flexneri, indicating that the S-EPS 2-1 was the specific polysaccharide in the spatial structure of biofilm formation. Our research found the important role of S-EPS in S. flexneri biofilm formation, which will help us to understand the underlining mechanisms of the biofilm formation and find effective ways to prevent S. flexneri biofilm infection.https://www.frontiersin.org/articles/10.3389/fmicb.2021.807397/fullShigella flexneripolysaccharidestructure identificationbiofilm formationmatrix interaction |
| spellingShingle | Yinglong Song Fenglian Ma Mengying Sun Guangqing Mu Guangqing Mu Yanfeng Tuo The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri Frontiers in Microbiology Shigella flexneri polysaccharide structure identification biofilm formation matrix interaction |
| title | The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri |
| title_full | The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri |
| title_fullStr | The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri |
| title_full_unstemmed | The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri |
| title_short | The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced by Shigella flexneri |
| title_sort | chemical structure properties and promoting biofilm activity of exopolysaccharide produced by shigella flexneri |
| topic | Shigella flexneri polysaccharide structure identification biofilm formation matrix interaction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.807397/full |
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