| Summary: | Oxidative stress-related conditions associated with lung cells, specifically lung cancer, often lead to a poor prognosis. We hypothesized that platinum nanoparticles (PtNPs) can play a role in reversing oxidative stress in human lung adenocarcinoma A549 epithelial lung cell lines. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was used to induce oxidative stress in cells, and the ability of PtNPs to lower the oxidative stress in the H<sub>2</sub>O<sub>2</sub> treated epithelial lung cell line was determined. The differential capacity of PtNPs to remove H<sub>2</sub>O<sub>2</sub> was studied through cell viability, nanoparticle uptake, DNA damage, ROS production, and antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). Results indicated that a higher concentration of PtNPs exhibited a higher antioxidant capacity and was able to reduce DNA damage and quench ROS production in the presence of 350 µM H<sub>2</sub>O<sub>2</sub>. All antioxidant enzymes’ activities also increased in the PtNPs treatment. Our data suggested that PtNPs could be a promising antioxidant in the treatment of lung cancer.
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