H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki
We determined 2H stable isotope fractionation at natural abundances associated with hydrogenase activity by whole cells of Desulfovibrio vulgaris strain Miyazaki F expressing a NiFe(Se) hydrogenase. Inhibition of sulfate reduction by molybdate inhibited the overall oxidation of hydrogen but still fa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01545/full |
| _version_ | 1818961547693654016 |
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| author | Michaela Löffler Steffen Kümmel Carsten Vogt Hans-Hermann Richnow |
| author_facet | Michaela Löffler Steffen Kümmel Carsten Vogt Hans-Hermann Richnow |
| author_sort | Michaela Löffler |
| collection | DOAJ |
| description | We determined 2H stable isotope fractionation at natural abundances associated with hydrogenase activity by whole cells of Desulfovibrio vulgaris strain Miyazaki F expressing a NiFe(Se) hydrogenase. Inhibition of sulfate reduction by molybdate inhibited the overall oxidation of hydrogen but still facilitated an equilibrium isotope exchange reaction with water. The theoretical equilibrium isotope exchange δ2H-values of the chemical exchange reaction were identical to the hydrogenase reaction, as confirmed using three isotopically different waters with δ2H-values of – 62, +461, and + 1533‰. Expected kinetic isotope fractionation of hydrogen oxidation by non-inhibited cells was also superimposed by an equilibrium isotope exchange. The isotope effects were solely catalyzed biotically as hydrogen isotope signatures did not change in control experiments without cells of D. vulgaris Miyazaki. |
| first_indexed | 2024-12-20T12:15:10Z |
| format | Article |
| id | doaj.art-aafbbb677e8d4ce6abb17479d2e68830 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T12:15:10Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aafbbb677e8d4ce6abb17479d2e688302022-12-21T19:41:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-07-011010.3389/fmicb.2019.01545468965H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain MiyazakiMichaela LöfflerSteffen KümmelCarsten VogtHans-Hermann RichnowWe determined 2H stable isotope fractionation at natural abundances associated with hydrogenase activity by whole cells of Desulfovibrio vulgaris strain Miyazaki F expressing a NiFe(Se) hydrogenase. Inhibition of sulfate reduction by molybdate inhibited the overall oxidation of hydrogen but still facilitated an equilibrium isotope exchange reaction with water. The theoretical equilibrium isotope exchange δ2H-values of the chemical exchange reaction were identical to the hydrogenase reaction, as confirmed using three isotopically different waters with δ2H-values of – 62, +461, and + 1533‰. Expected kinetic isotope fractionation of hydrogen oxidation by non-inhibited cells was also superimposed by an equilibrium isotope exchange. The isotope effects were solely catalyzed biotically as hydrogen isotope signatures did not change in control experiments without cells of D. vulgaris Miyazaki.https://www.frontiersin.org/article/10.3389/fmicb.2019.01545/fullhydrogenaseD. vulgaris strain MiyazakimonitoringGC-IRMSequilibrium isotope fractionationkinetic isotope fractionation |
| spellingShingle | Michaela Löffler Steffen Kümmel Carsten Vogt Hans-Hermann Richnow H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki Frontiers in Microbiology hydrogenase D. vulgaris strain Miyazaki monitoring GC-IRMS equilibrium isotope fractionation kinetic isotope fractionation |
| title | H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki |
| title_full | H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki |
| title_fullStr | H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki |
| title_full_unstemmed | H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki |
| title_short | H2 Kinetic Isotope Fractionation Superimposed by Equilibrium Isotope Fractionation During Hydrogenase Activity of D. vulgaris Strain Miyazaki |
| title_sort | h2 kinetic isotope fractionation superimposed by equilibrium isotope fractionation during hydrogenase activity of d vulgaris strain miyazaki |
| topic | hydrogenase D. vulgaris strain Miyazaki monitoring GC-IRMS equilibrium isotope fractionation kinetic isotope fractionation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01545/full |
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