Improving Dispersion of Bacterial Endospores for Enumeration
Precise enumeration of spores is crucial for accurate evaluation of spore survival in the presence of inactivating agents or extreme environmental conditions. Bacterial endospores tend to agglomerate, leading to low estimates of spore numbers. We have shown that addition of SDS to diluent used in co...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hosokawa Powder Technology Foundation
2015-09-01
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| Series: | KONA Powder and Particle Journal |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/kona/33/0/33_2016015/_html/-char/en |
| _version_ | 1818332952805769216 |
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| author | Vijay B. Krishna Jue Zhao Smithi Pumprueg Ben L. Koopman Brij M. Moudgil |
| author_facet | Vijay B. Krishna Jue Zhao Smithi Pumprueg Ben L. Koopman Brij M. Moudgil |
| author_sort | Vijay B. Krishna |
| collection | DOAJ |
| description | Precise enumeration of spores is crucial for accurate evaluation of spore survival in the presence of inactivating agents or extreme environmental conditions. Bacterial endospores tend to agglomerate, leading to low estimates of spore numbers. We have shown that addition of SDS to diluent used in counting bacterial endospores better disperses spores, leading to a ten-fold increase in counts. We attribute this effect to steric hindrance and electrostatic repulsion between micellar structures of SDS adsorbed at spore surfaces. We have also demonstrated that use of a low surface energy material (Teflon) improves spread-plate counts, an effect we attribute to lesser hold-up of liquid on Teflon versus glass. |
| first_indexed | 2024-12-13T13:43:55Z |
| format | Article |
| id | doaj.art-aafc736c98854760a388cbe59a90261a |
| institution | Directory Open Access Journal |
| issn | 0288-4534 2187-5537 |
| language | English |
| last_indexed | 2024-12-13T13:43:55Z |
| publishDate | 2015-09-01 |
| publisher | Hosokawa Powder Technology Foundation |
| record_format | Article |
| series | KONA Powder and Particle Journal |
| spelling | doaj.art-aafc736c98854760a388cbe59a90261a2022-12-21T23:43:30ZengHosokawa Powder Technology FoundationKONA Powder and Particle Journal0288-45342187-55372015-09-0133030430910.14356/kona.2016015konaImproving Dispersion of Bacterial Endospores for EnumerationVijay B. Krishna0Jue Zhao1Smithi Pumprueg2Ben L. Koopman3Brij M. Moudgil4Particle Engineering Research Center, Department of Materials Science and Engineering, University of Florida, USAParticle Engineering Research Center, Department of Environmental Engineering Sciences, University of Florida, USAParticle Engineering Research Center, Department of Environmental Engineering Sciences, University of Florida, USAParticle Engineering Research Center, Department of Environmental Engineering Sciences, University of Florida, USAParticle Engineering Research Center, Department of Materials Science and Engineering, University of Florida, USAPrecise enumeration of spores is crucial for accurate evaluation of spore survival in the presence of inactivating agents or extreme environmental conditions. Bacterial endospores tend to agglomerate, leading to low estimates of spore numbers. We have shown that addition of SDS to diluent used in counting bacterial endospores better disperses spores, leading to a ten-fold increase in counts. We attribute this effect to steric hindrance and electrostatic repulsion between micellar structures of SDS adsorbed at spore surfaces. We have also demonstrated that use of a low surface energy material (Teflon) improves spread-plate counts, an effect we attribute to lesser hold-up of liquid on Teflon versus glass.https://www.jstage.jst.go.jp/article/kona/33/0/33_2016015/_html/-char/ensporeenumerationplatingagglomerationsurfactantteflon |
| spellingShingle | Vijay B. Krishna Jue Zhao Smithi Pumprueg Ben L. Koopman Brij M. Moudgil Improving Dispersion of Bacterial Endospores for Enumeration KONA Powder and Particle Journal spore enumeration plating agglomeration surfactant teflon |
| title | Improving Dispersion of Bacterial Endospores for Enumeration |
| title_full | Improving Dispersion of Bacterial Endospores for Enumeration |
| title_fullStr | Improving Dispersion of Bacterial Endospores for Enumeration |
| title_full_unstemmed | Improving Dispersion of Bacterial Endospores for Enumeration |
| title_short | Improving Dispersion of Bacterial Endospores for Enumeration |
| title_sort | improving dispersion of bacterial endospores for enumeration |
| topic | spore enumeration plating agglomeration surfactant teflon |
| url | https://www.jstage.jst.go.jp/article/kona/33/0/33_2016015/_html/-char/en |
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