Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines
In this study, the composition and richness of bacterial communities in treated and untreated wastewater from hospitals, commercial, and non-commercial fish farming sites, sewage effluents, and surface waters, which included seawater and fresh water in Sri Lanka and the Philippines, were investigate...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Water |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frwa.2021.730124/full |
| _version_ | 1818574547900694528 |
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| author | Andre Freire Cruz R. G. S. Wijesekara K. B. S. N. Jinadasa Benjamin J. Gonzales Takeshi Ohura Keerthi S. Guruge Keerthi S. Guruge Keerthi S. Guruge |
| author_facet | Andre Freire Cruz R. G. S. Wijesekara K. B. S. N. Jinadasa Benjamin J. Gonzales Takeshi Ohura Keerthi S. Guruge Keerthi S. Guruge Keerthi S. Guruge |
| author_sort | Andre Freire Cruz |
| collection | DOAJ |
| description | In this study, the composition and richness of bacterial communities in treated and untreated wastewater from hospitals, commercial, and non-commercial fish farming sites, sewage effluents, and surface waters, which included seawater and fresh water in Sri Lanka and the Philippines, were investigated through 16S rRNA gene amplicon sequence analysis. Firmicutes were found predominantly in Sri Lankan hospital wastewaters, while Cyanobacteria and Acidobacteria were typically detected in fish culture sites and the waste canal in Sri Lanka, respectively. The Shannon–Weaver index (SW) and number of Operational Taxonomic Units (OTUs) were higher in the Philippines than in Sri Lanka. The bacterial richness in the university non-commercial fish pond and sewage effluent displayed greater than that in hospital wastewaters. In addition, the bacterial richness was higher in the untreated wastewater compared to that in the treated wastewater in hospitals. These results indicate the differences among water types in terms of bacterial community, especially influenced by their source. |
| first_indexed | 2024-12-15T00:28:00Z |
| format | Article |
| id | doaj.art-aafd8caf71184b25859de5bd0ec429d4 |
| institution | Directory Open Access Journal |
| issn | 2624-9375 |
| language | English |
| last_indexed | 2024-12-15T00:28:00Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Water |
| spelling | doaj.art-aafd8caf71184b25859de5bd0ec429d42022-12-21T22:42:07ZengFrontiers Media S.A.Frontiers in Water2624-93752021-12-01310.3389/frwa.2021.730124730124Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the PhilippinesAndre Freire Cruz0R. G. S. Wijesekara1K. B. S. N. Jinadasa2Benjamin J. Gonzales3Takeshi Ohura4Keerthi S. Guruge5Keerthi S. Guruge6Keerthi S. Guruge7Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanDepartment of Aquaculture and Fisheries, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Sri LankaDepartment of Civil Engineering, University of Peradeniya, Peradeniya, Sri LankaCollege of Fisheries and Aquatic Sciences, Western Philippines University Puerto Princesa Campus, Puerto Princesa, PhilippinesGraduate School of Agriculture, Meijo University, Nagoya, JapanNational Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, JapanNational Institute of Fundamental Studies, Kandy, Sri LankaIn this study, the composition and richness of bacterial communities in treated and untreated wastewater from hospitals, commercial, and non-commercial fish farming sites, sewage effluents, and surface waters, which included seawater and fresh water in Sri Lanka and the Philippines, were investigated through 16S rRNA gene amplicon sequence analysis. Firmicutes were found predominantly in Sri Lankan hospital wastewaters, while Cyanobacteria and Acidobacteria were typically detected in fish culture sites and the waste canal in Sri Lanka, respectively. The Shannon–Weaver index (SW) and number of Operational Taxonomic Units (OTUs) were higher in the Philippines than in Sri Lanka. The bacterial richness in the university non-commercial fish pond and sewage effluent displayed greater than that in hospital wastewaters. In addition, the bacterial richness was higher in the untreated wastewater compared to that in the treated wastewater in hospitals. These results indicate the differences among water types in terms of bacterial community, especially influenced by their source.https://www.frontiersin.org/articles/10.3389/frwa.2021.730124/fullbacterial communityhospital wastewaterfish culture sitessurface water16S rRNA (16S rDNA) |
| spellingShingle | Andre Freire Cruz R. G. S. Wijesekara K. B. S. N. Jinadasa Benjamin J. Gonzales Takeshi Ohura Keerthi S. Guruge Keerthi S. Guruge Keerthi S. Guruge Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines Frontiers in Water bacterial community hospital wastewater fish culture sites surface water 16S rRNA (16S rDNA) |
| title | Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines |
| title_full | Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines |
| title_fullStr | Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines |
| title_full_unstemmed | Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines |
| title_short | Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines |
| title_sort | preliminary investigation of microbial community in wastewater and surface waters in sri lanka and the philippines |
| topic | bacterial community hospital wastewater fish culture sites surface water 16S rRNA (16S rDNA) |
| url | https://www.frontiersin.org/articles/10.3389/frwa.2021.730124/full |
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