High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i>
High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in <i>Populus canescens</i>. However, the mechanism by which high temperature induces pollen abortion remains unknown. Here, pollen abortion was induced by exposing male flower buds of &l...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/4/797 |
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| author | Zhiqun Li Yifan Zhao Xuetong Cheng Bo Kong Yaru Sang Qing Zhou Jian Wu Pingdong Zhang |
| author_facet | Zhiqun Li Yifan Zhao Xuetong Cheng Bo Kong Yaru Sang Qing Zhou Jian Wu Pingdong Zhang |
| author_sort | Zhiqun Li |
| collection | DOAJ |
| description | High temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in <i>Populus canescens</i>. However, the mechanism by which high temperature induces pollen abortion remains unknown. Here, pollen abortion was induced by exposing male flower buds of <i>P. canescens</i> to 38 and 41 °C; pollen morphology, meiotic abnormalities, defects of the meiotic microtubular cytoskeleton, and tapetum development were characterized. We found that the dominant meiotic stage, temperature, and duration of treatment significantly affected the percentage of high temperature-induced aborted pollen. Damaged spindle microtubules and depolymerized microtubular cytoskeletons were observed, which resulted in many lagging chromosomes at anaphase I and II as well as aneuploid male gametes and micronuclei, thereby generating aborted pollen grains. Tapetum disintegration was also delayed. The anther dehisced normally, and some viable pollen grains were released. However, no sufficient evidence showed that the delayed degradation of the tapetum was responsible for pollen abortion. |
| first_indexed | 2024-03-11T05:01:21Z |
| format | Article |
| id | doaj.art-ab05f1ad36ea48598a43fe7bf6b23b0e |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T05:01:21Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Forests |
| spelling | doaj.art-ab05f1ad36ea48598a43fe7bf6b23b0e2023-11-17T19:18:01ZengMDPI AGForests1999-49072023-04-0114479710.3390/f14040797High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i>Zhiqun Li0Yifan Zhao1Xuetong Cheng2Bo Kong3Yaru Sang4Qing Zhou5Jian Wu6Pingdong Zhang7State Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaState Key Laboratory of Efficient Production of Forest Tree Resources, Beijing Forestry University, Beijing 100083, ChinaHigh temperature can induce the production of 2n gametes and aborted pollen during microsporogenesis in <i>Populus canescens</i>. However, the mechanism by which high temperature induces pollen abortion remains unknown. Here, pollen abortion was induced by exposing male flower buds of <i>P. canescens</i> to 38 and 41 °C; pollen morphology, meiotic abnormalities, defects of the meiotic microtubular cytoskeleton, and tapetum development were characterized. We found that the dominant meiotic stage, temperature, and duration of treatment significantly affected the percentage of high temperature-induced aborted pollen. Damaged spindle microtubules and depolymerized microtubular cytoskeletons were observed, which resulted in many lagging chromosomes at anaphase I and II as well as aneuploid male gametes and micronuclei, thereby generating aborted pollen grains. Tapetum disintegration was also delayed. The anther dehisced normally, and some viable pollen grains were released. However, no sufficient evidence showed that the delayed degradation of the tapetum was responsible for pollen abortion.https://www.mdpi.com/1999-4907/14/4/797high temperatureaborted pollenspindlemicrotubular cytoskeletontapetum<i>Populus canescens</i> |
| spellingShingle | Zhiqun Li Yifan Zhao Xuetong Cheng Bo Kong Yaru Sang Qing Zhou Jian Wu Pingdong Zhang High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> Forests high temperature aborted pollen spindle microtubular cytoskeleton tapetum <i>Populus canescens</i> |
| title | High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> |
| title_full | High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> |
| title_fullStr | High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> |
| title_full_unstemmed | High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> |
| title_short | High Temperature-Induced Spindle Destabilization Results in Aborted Pollen Production in <i>Populus</i> |
| title_sort | high temperature induced spindle destabilization results in aborted pollen production in i populus i |
| topic | high temperature aborted pollen spindle microtubular cytoskeleton tapetum <i>Populus canescens</i> |
| url | https://www.mdpi.com/1999-4907/14/4/797 |
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