Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli
Abstract Background Poultry remains one of the most important reservoir for zoonotic multidrug resistant pathogens. The global rise of antimicrobial resistance in Gram-negative bacteria is of reasonable concern and demands intensified surveillance. Methods In 2016, 576 cloacal swabs were collected f...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-09-01
|
| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13099-018-0266-5 |
| _version_ | 1818014297035374592 |
|---|---|
| author | Amira A. Moawad Helmut Hotzel Heinrich Neubauer Ralf Ehricht Stefan Monecke Herbert Tomaso Hafez M. Hafez Uwe Roesler Hosny El-Adawy |
| author_facet | Amira A. Moawad Helmut Hotzel Heinrich Neubauer Ralf Ehricht Stefan Monecke Herbert Tomaso Hafez M. Hafez Uwe Roesler Hosny El-Adawy |
| author_sort | Amira A. Moawad |
| collection | DOAJ |
| description | Abstract Background Poultry remains one of the most important reservoir for zoonotic multidrug resistant pathogens. The global rise of antimicrobial resistance in Gram-negative bacteria is of reasonable concern and demands intensified surveillance. Methods In 2016, 576 cloacal swabs were collected from 48 broiler farms located in five governorates in northern Egypt. Isolates of Enterobacteriaceae could be cultivated on different media and were identified by MALDI-TOF MS and PCR. Escherichia coli isolates were genotyped by DNA-microarray-based assays. The antimicrobial susceptibility to 14 antibiotics was determined and resistance-associated genes were detected. The VITEK-2 system was applied for phenotypical confirmation of extended-spectrum β-lactamase-producing isolates. The determination of colistin resistance was carried out phenotypically using E-test and genotypically using PCR for detection of the mcr-1 gene. Results Out of 576 samples, 72 representatives of Enterobacteriaceae were isolated and identified as 63 E. coli (87.5%), 5 Enterobacter cloacae (6.9%), 2 Klebsiella pneumoniae (2.8%) and 2 Citrobacter spp. (2.8%). Seven out of 56 cultivated E. coli (12.5%) were confirmed as ESBL-producing E. coli and one isolate (1.8%) as ESBL/carbapenemase-producing E. coli. Five out of 63 E. coli isolates (7.9%) recovered from different poultry flocks were phenotypically resistant to colistin and harboured mcr-1 gene. Conclusions This is the first study reporting colistin resistance and emergence of multidrug resistance in Enterobacteriaceae isolated from healthy broilers in the Nile Delta region, Egypt. Colistin-resistant E. coli in poultry is of public health significance. The global rise of ESBL- and carbapenemase-producing Gram-negative bacteria demands intensified surveillance. ESBL-producing E. coli in poultry farms in Egypt are of major concern that emphasizes the possibility of spread of such strains to humans. The results also reinforce the need to develop strategies and to implement specific control procedures to reduce the use of antibiotics. |
| first_indexed | 2024-04-14T06:43:11Z |
| format | Article |
| id | doaj.art-ab060c39dbe14806b50e82c5664a085e |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-04-14T06:43:11Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-ab060c39dbe14806b50e82c5664a085e2022-12-22T02:07:15ZengBMCGut Pathogens1757-47492018-09-0110111210.1186/s13099-018-0266-5Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coliAmira A. Moawad0Helmut Hotzel1Heinrich Neubauer2Ralf Ehricht3Stefan Monecke4Herbert Tomaso5Hafez M. Hafez6Uwe Roesler7Hosny El-Adawy8Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and ZoonosesFriedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and ZoonosesFriedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and ZoonosesAlere Technologies GmbHAlere Technologies GmbHFriedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and ZoonosesInstitute for Poultry Diseases, Free University BerlinInstitute for Animal Hygiene and Environmental Health, Free University BerlinFriedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and ZoonosesAbstract Background Poultry remains one of the most important reservoir for zoonotic multidrug resistant pathogens. The global rise of antimicrobial resistance in Gram-negative bacteria is of reasonable concern and demands intensified surveillance. Methods In 2016, 576 cloacal swabs were collected from 48 broiler farms located in five governorates in northern Egypt. Isolates of Enterobacteriaceae could be cultivated on different media and were identified by MALDI-TOF MS and PCR. Escherichia coli isolates were genotyped by DNA-microarray-based assays. The antimicrobial susceptibility to 14 antibiotics was determined and resistance-associated genes were detected. The VITEK-2 system was applied for phenotypical confirmation of extended-spectrum β-lactamase-producing isolates. The determination of colistin resistance was carried out phenotypically using E-test and genotypically using PCR for detection of the mcr-1 gene. Results Out of 576 samples, 72 representatives of Enterobacteriaceae were isolated and identified as 63 E. coli (87.5%), 5 Enterobacter cloacae (6.9%), 2 Klebsiella pneumoniae (2.8%) and 2 Citrobacter spp. (2.8%). Seven out of 56 cultivated E. coli (12.5%) were confirmed as ESBL-producing E. coli and one isolate (1.8%) as ESBL/carbapenemase-producing E. coli. Five out of 63 E. coli isolates (7.9%) recovered from different poultry flocks were phenotypically resistant to colistin and harboured mcr-1 gene. Conclusions This is the first study reporting colistin resistance and emergence of multidrug resistance in Enterobacteriaceae isolated from healthy broilers in the Nile Delta region, Egypt. Colistin-resistant E. coli in poultry is of public health significance. The global rise of ESBL- and carbapenemase-producing Gram-negative bacteria demands intensified surveillance. ESBL-producing E. coli in poultry farms in Egypt are of major concern that emphasizes the possibility of spread of such strains to humans. The results also reinforce the need to develop strategies and to implement specific control procedures to reduce the use of antibiotics.http://link.springer.com/article/10.1186/s13099-018-0266-5EnterobacteriaceaeAntibiotic resistanceDNA microarrayESBLColistinBroiler |
| spellingShingle | Amira A. Moawad Helmut Hotzel Heinrich Neubauer Ralf Ehricht Stefan Monecke Herbert Tomaso Hafez M. Hafez Uwe Roesler Hosny El-Adawy Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli Gut Pathogens Enterobacteriaceae Antibiotic resistance DNA microarray ESBL Colistin Broiler |
| title | Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli |
| title_full | Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli |
| title_fullStr | Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli |
| title_full_unstemmed | Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli |
| title_short | Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli |
| title_sort | antimicrobial resistance in enterobacteriaceae from healthy broilers in egypt emergence of colistin resistant and extended spectrum β lactamase producing escherichia coli |
| topic | Enterobacteriaceae Antibiotic resistance DNA microarray ESBL Colistin Broiler |
| url | http://link.springer.com/article/10.1186/s13099-018-0266-5 |
| work_keys_str_mv | AT amiraamoawad antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT helmuthotzel antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT heinrichneubauer antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT ralfehricht antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT stefanmonecke antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT herberttomaso antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT hafezmhafez antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT uweroesler antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli AT hosnyeladawy antimicrobialresistanceinenterobacteriaceaefromhealthybroilersinegyptemergenceofcolistinresistantandextendedspectrumblactamaseproducingescherichiacoli |