An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth
Abstract Microprobes are used to repair neuronal injury by recording electrical signals from neuronal cells around the surface of the device. Following implantation into the brain, the immune response results in formation of scar tissue around the microprobe. However, neurons must be in close proxim...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-12-01
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| Series: | Micro and Nano Systems Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40486-017-0062-x |
| _version_ | 1818910974928748544 |
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| author | Eunhee Kim Jin-Young Kim Hongsoo Choi |
| author_facet | Eunhee Kim Jin-Young Kim Hongsoo Choi |
| author_sort | Eunhee Kim |
| collection | DOAJ |
| description | Abstract Microprobes are used to repair neuronal injury by recording electrical signals from neuronal cells around the surface of the device. Following implantation into the brain, the immune response results in formation of scar tissue around the microprobe. However, neurons must be in close proximity to the microprobe to enable signal recording. A common reason for failure of microprobes is impaired signal recording due to scar tissue, which is not related to the microprobe itself. Therefore, the device–cell interface must be improved to increase the number of neurons in contact with the surface. In this study, we developed nanostructured SU-8 microprobes to support neuronal growth. Nanostructures of 200 nm diameter and depth were applied to the surface of microprobes, and the attachment and neurite outgrowth of PC12 cells on the microprobes were evaluated. Neuronal attachment and neurite outgrowth on the nanostructured microprobes were significantly greater than those on non-nanostructured microprobes. The enhanced neuronal attachment and neurite outgrowth on the nanostructured microprobes occurred in the absence of an adhesive coating, such as poly-l-lysine, and so may be useful for implantable devices for long-term use. Therefore, nanostructured microprobes can be implanted without adhesive coating, which can cause problems in vivo over the long term. |
| first_indexed | 2024-12-19T22:51:20Z |
| format | Article |
| id | doaj.art-ab0d2a2e947443ed8c9476d4032735f1 |
| institution | Directory Open Access Journal |
| issn | 2213-9621 |
| language | English |
| last_indexed | 2024-12-19T22:51:20Z |
| publishDate | 2017-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Micro and Nano Systems Letters |
| spelling | doaj.art-ab0d2a2e947443ed8c9476d4032735f12022-12-21T20:02:48ZengSpringerOpenMicro and Nano Systems Letters2213-96212017-12-01511810.1186/s40486-017-0062-xAn SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growthEunhee Kim0Jin-Young Kim1Hongsoo Choi2DGIST-ETH Microrobotics Research Center (DEMRC), Daegu Gyeongbuk Institute of Science and Technology (DGIST)DGIST-ETH Microrobotics Research Center (DEMRC), Daegu Gyeongbuk Institute of Science and Technology (DGIST)DGIST-ETH Microrobotics Research Center (DEMRC), Daegu Gyeongbuk Institute of Science and Technology (DGIST)Abstract Microprobes are used to repair neuronal injury by recording electrical signals from neuronal cells around the surface of the device. Following implantation into the brain, the immune response results in formation of scar tissue around the microprobe. However, neurons must be in close proximity to the microprobe to enable signal recording. A common reason for failure of microprobes is impaired signal recording due to scar tissue, which is not related to the microprobe itself. Therefore, the device–cell interface must be improved to increase the number of neurons in contact with the surface. In this study, we developed nanostructured SU-8 microprobes to support neuronal growth. Nanostructures of 200 nm diameter and depth were applied to the surface of microprobes, and the attachment and neurite outgrowth of PC12 cells on the microprobes were evaluated. Neuronal attachment and neurite outgrowth on the nanostructured microprobes were significantly greater than those on non-nanostructured microprobes. The enhanced neuronal attachment and neurite outgrowth on the nanostructured microprobes occurred in the absence of an adhesive coating, such as poly-l-lysine, and so may be useful for implantable devices for long-term use. Therefore, nanostructured microprobes can be implanted without adhesive coating, which can cause problems in vivo over the long term.http://link.springer.com/article/10.1186/s40486-017-0062-xSurface modificationNanostructured surfaceNanosphere lithographyCell attachmentNeurite outgrowth |
| spellingShingle | Eunhee Kim Jin-Young Kim Hongsoo Choi An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth Micro and Nano Systems Letters Surface modification Nanostructured surface Nanosphere lithography Cell attachment Neurite outgrowth |
| title | An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| title_full | An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| title_fullStr | An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| title_full_unstemmed | An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| title_short | An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| title_sort | su 8 based microprobe with a nanostructured surface enhances neuronal cell attachment and growth |
| topic | Surface modification Nanostructured surface Nanosphere lithography Cell attachment Neurite outgrowth |
| url | http://link.springer.com/article/10.1186/s40486-017-0062-x |
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