Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken
ABSTRACT: Inosine monophosphate (IMP) plays a significant role in meat taste, yet the molecular mechanisms controlling IMP deposition in muscle tissues still require elucidation. The present study systematically and comprehensively explores the molecular network governing IMP deposition in different...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123006375 |
| _version_ | 1797401837448462336 |
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| author | Zengwen Huang Zhengyun Cai Juan Zhang Yaling Gu Jing Wang Jinzeng Yang Gang Lv Chaoyun Yang Yi Zhang Chen Ji Shengwang Jiang |
| author_facet | Zengwen Huang Zhengyun Cai Juan Zhang Yaling Gu Jing Wang Jinzeng Yang Gang Lv Chaoyun Yang Yi Zhang Chen Ji Shengwang Jiang |
| author_sort | Zengwen Huang |
| collection | DOAJ |
| description | ABSTRACT: Inosine monophosphate (IMP) plays a significant role in meat taste, yet the molecular mechanisms controlling IMP deposition in muscle tissues still require elucidation. The present study systematically and comprehensively explores the molecular network governing IMP deposition in different regions of Jingyuan chicken muscle. Two muscle groups, the breast and leg, were examined as test materials. Using nontargeted metabolomic sequencing, we screened and identified 20 metabolites that regulate IMP-specific deposition. We maintained regular author and institution formatting, used clear, objective, and value-neutral language, and avoided biased or emotional language. We followed a consistent footnote style and formatting features and used precise word choice with technical terms where appropriate. Out of these, 5 were identified as significant contributors to the regulation of IMP deposition. We explained technical term abbreviations when first used and ensured a logical flow of information with causal connections between statements. The results indicate that PGM1, a key enzyme involved in synthesis, is higher in the breast muscle compared to the leg muscle, which may provide an explanation for the increased deposition of IMP in the breast muscle. We aimed for a clear structure with logical progression, avoided filler words, and ensured grammatical correctness. The activity of key enzymes (PKM2, AK1, AMPD1) involved in this process was higher in the breast muscle than in the leg muscle. In the case of IMP degradation metabolism, the activity of its participating enzyme (PurH) was lower in the breast muscle than in the leg muscle. These findings suggest that the increased deposition of IMP in Jingyuan chickens' breast muscle may result from elevated metabolism and reduced catabolism of key metabolites. In summary, a metaomic strategy was utilized to assess the molecular network regulation mechanism of IMP-specific deposition in various segments of Jingyuan chicken. These findings provide insight into genetic improvement and molecular breeding of meat quality traits for top-notch broilers. |
| first_indexed | 2024-03-09T02:16:05Z |
| format | Article |
| id | doaj.art-ab0dc56e3bd64e90a890d503af726a34 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-09T02:16:05Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-ab0dc56e3bd64e90a890d503af726a342023-12-07T05:27:16ZengElsevierPoultry Science0032-57912023-12-0110212103118Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chickenZengwen Huang0Zhengyun Cai1Juan Zhang2Yaling Gu3Jing Wang4Jinzeng Yang5Gang Lv6Chaoyun Yang7Yi Zhang8Chen Ji9Shengwang Jiang10Agriculture College, Ningxia University, Ningxia, Yinchuan 750021, China; College of Animal Science, Xichang University, Sichuan, Xichang 615012, China; Xinjiang Taikun Group Co., Ltd., Xinjiang, Changji 831100, ChinaAgriculture College, Ningxia University, Ningxia, Yinchuan 750021, ChinaAgriculture College, Ningxia University, Ningxia, Yinchuan 750021, China; Corresponding author:Agriculture College, Ningxia University, Ningxia, Yinchuan 750021, ChinaCollege of Animal Science, Xichang University, Sichuan, Xichang 615012, ChinaDepartment of Human Nutrition, Food & Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Manoa, HI 96822Xinjiang Taikun Group Co., Ltd., Xinjiang, Changji 831100, ChinaCollege of Animal Science, Xichang University, Sichuan, Xichang 615012, ChinaCollege of Animal Science, Xichang University, Sichuan, Xichang 615012, ChinaCollege of Animal Science, Xichang University, Sichuan, Xichang 615012, ChinaCollege of Animal Science, Xichang University, Sichuan, Xichang 615012, ChinaABSTRACT: Inosine monophosphate (IMP) plays a significant role in meat taste, yet the molecular mechanisms controlling IMP deposition in muscle tissues still require elucidation. The present study systematically and comprehensively explores the molecular network governing IMP deposition in different regions of Jingyuan chicken muscle. Two muscle groups, the breast and leg, were examined as test materials. Using nontargeted metabolomic sequencing, we screened and identified 20 metabolites that regulate IMP-specific deposition. We maintained regular author and institution formatting, used clear, objective, and value-neutral language, and avoided biased or emotional language. We followed a consistent footnote style and formatting features and used precise word choice with technical terms where appropriate. Out of these, 5 were identified as significant contributors to the regulation of IMP deposition. We explained technical term abbreviations when first used and ensured a logical flow of information with causal connections between statements. The results indicate that PGM1, a key enzyme involved in synthesis, is higher in the breast muscle compared to the leg muscle, which may provide an explanation for the increased deposition of IMP in the breast muscle. We aimed for a clear structure with logical progression, avoided filler words, and ensured grammatical correctness. The activity of key enzymes (PKM2, AK1, AMPD1) involved in this process was higher in the breast muscle than in the leg muscle. In the case of IMP degradation metabolism, the activity of its participating enzyme (PurH) was lower in the breast muscle than in the leg muscle. These findings suggest that the increased deposition of IMP in Jingyuan chickens' breast muscle may result from elevated metabolism and reduced catabolism of key metabolites. In summary, a metaomic strategy was utilized to assess the molecular network regulation mechanism of IMP-specific deposition in various segments of Jingyuan chicken. These findings provide insight into genetic improvement and molecular breeding of meat quality traits for top-notch broilers.http://www.sciencedirect.com/science/article/pii/S0032579123006375IMPJingyuan chickenintegromicsproteomicsmetabonomics |
| spellingShingle | Zengwen Huang Zhengyun Cai Juan Zhang Yaling Gu Jing Wang Jinzeng Yang Gang Lv Chaoyun Yang Yi Zhang Chen Ji Shengwang Jiang Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken Poultry Science IMP Jingyuan chicken integromics proteomics metabonomics |
| title | Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken |
| title_full | Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken |
| title_fullStr | Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken |
| title_full_unstemmed | Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken |
| title_short | Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken |
| title_sort | integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate specific deposition in jingyuan chicken |
| topic | IMP Jingyuan chicken integromics proteomics metabonomics |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123006375 |
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