The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis
This study examined the effect of sleep disturbance on gut microbiota (GM), atrial substrate, and atrial fibrillation (AF) inducibility. C57BL/6 mice were subjected to six weeks of sleep deprivation (SD) using the method of modified multiple-platform. Transesophageal burst pacing was performed to ev...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/11/1144 |
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| author | Kun Zuo Chen Fang Yuan Fu Zheng Liu Ye Liu Lifeng Liu Yuxing Wang Hongjiang Wang Xiandong Yin Xiaoqing Liu Jing Li Jiuchang Zhong Mulei Chen Xinchun Yang Li Xu |
| author_facet | Kun Zuo Chen Fang Yuan Fu Zheng Liu Ye Liu Lifeng Liu Yuxing Wang Hongjiang Wang Xiandong Yin Xiaoqing Liu Jing Li Jiuchang Zhong Mulei Chen Xinchun Yang Li Xu |
| author_sort | Kun Zuo |
| collection | DOAJ |
| description | This study examined the effect of sleep disturbance on gut microbiota (GM), atrial substrate, and atrial fibrillation (AF) inducibility. C57BL/6 mice were subjected to six weeks of sleep deprivation (SD) using the method of modified multiple-platform. Transesophageal burst pacing was performed to evaluate AF inducibility. Feces, plasma, and an atrium were collected and analyzed by 16s rRNA sequencing, liquid chromatography–mass spectrometry (LC-MS)-based metabolome, histological studies, and transcriptome. Higher AF inducibility (2/30 of control vs. 15/30 of SD, <i>p</i> = 0.001) and longer AF duration (<i>p</i> < 0.001), concomitant with aggravated fibrosis, collagen, and lipid accumulation, were seen in the SD mice compared to control mice. Meanwhile, elevated alpha diversity, higher abundance of <i>Flavonifractor</i>, <i>Ruminococcus,</i> and <i>Alloprevotella</i>, as well as imbalanced functional pathways, were observed in the gut of SD mice. Moreover, the global patterns for the plasma metabolome were altered, e.g., the decreased butanoate metabolism intermediates in SD mice. In addition, disrupted metabolic homeostasis in the SD atrium, such as fatty acid metabolism, was analyzed by the transcriptome. These results demonstrated that the crosstalk between GM and atrial metabolism might be a promising target for SD-mediated AF susceptibility. |
| first_indexed | 2024-03-09T18:09:09Z |
| format | Article |
| id | doaj.art-ab0e93bf4e0a44ae91e0f13ea6e98cf9 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T18:09:09Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-ab0e93bf4e0a44ae91e0f13ea6e98cf92023-11-24T09:13:46ZengMDPI AGMetabolites2218-19892022-11-011211114410.3390/metabo12111144The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics AnalysisKun Zuo0Chen Fang1Yuan Fu2Zheng Liu3Ye Liu4Lifeng Liu5Yuxing Wang6Hongjiang Wang7Xiandong Yin8Xiaoqing Liu9Jing Li10Jiuchang Zhong11Mulei Chen12Xinchun Yang13Li Xu14Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaHeart Center and Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, ChinaThis study examined the effect of sleep disturbance on gut microbiota (GM), atrial substrate, and atrial fibrillation (AF) inducibility. C57BL/6 mice were subjected to six weeks of sleep deprivation (SD) using the method of modified multiple-platform. Transesophageal burst pacing was performed to evaluate AF inducibility. Feces, plasma, and an atrium were collected and analyzed by 16s rRNA sequencing, liquid chromatography–mass spectrometry (LC-MS)-based metabolome, histological studies, and transcriptome. Higher AF inducibility (2/30 of control vs. 15/30 of SD, <i>p</i> = 0.001) and longer AF duration (<i>p</i> < 0.001), concomitant with aggravated fibrosis, collagen, and lipid accumulation, were seen in the SD mice compared to control mice. Meanwhile, elevated alpha diversity, higher abundance of <i>Flavonifractor</i>, <i>Ruminococcus,</i> and <i>Alloprevotella</i>, as well as imbalanced functional pathways, were observed in the gut of SD mice. Moreover, the global patterns for the plasma metabolome were altered, e.g., the decreased butanoate metabolism intermediates in SD mice. In addition, disrupted metabolic homeostasis in the SD atrium, such as fatty acid metabolism, was analyzed by the transcriptome. These results demonstrated that the crosstalk between GM and atrial metabolism might be a promising target for SD-mediated AF susceptibility.https://www.mdpi.com/2218-1989/12/11/1144sleep deprivationatrial fibrillationgut microbiotatranscriptomemetabolome |
| spellingShingle | Kun Zuo Chen Fang Yuan Fu Zheng Liu Ye Liu Lifeng Liu Yuxing Wang Hongjiang Wang Xiandong Yin Xiaoqing Liu Jing Li Jiuchang Zhong Mulei Chen Xinchun Yang Li Xu The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis Metabolites sleep deprivation atrial fibrillation gut microbiota transcriptome metabolome |
| title | The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis |
| title_full | The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis |
| title_fullStr | The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis |
| title_full_unstemmed | The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis |
| title_short | The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis |
| title_sort | impact of sleep disturbance on gut microbiota atrial substrate and atrial fibrillation inducibility in mice a multi omics analysis |
| topic | sleep deprivation atrial fibrillation gut microbiota transcriptome metabolome |
| url | https://www.mdpi.com/2218-1989/12/11/1144 |
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