Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis
Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturall...
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| Format: | Article |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.756764/full |
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| author | Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li |
| author_facet | Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li |
| author_sort | Kunli Zhang |
| collection | DOAJ |
| description | Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection. |
| first_indexed | 2024-12-17T20:50:28Z |
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| id | doaj.art-ab112a9b631c4b4d857f3c178494f140 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-17T20:50:28Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ab112a9b631c4b4d857f3c178494f1402022-12-21T21:33:03ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-11-01810.3389/fvets.2021.756764756764Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuisKunli Zhang0Kunli Zhang1Pinpin Chu2Pinpin Chu3Shuai Song4Shuai Song5Dongxia Yang6Dongxia Yang7Zhibiao Bian8Zhibiao Bian9Yan Li10Yan Li11Hongchao Gou12Hongchao Gou13Zhiyong Jiang14Zhiyong Jiang15Rujian Cai16Rujian Cai17Chunling Li18Chunling Li19Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaInstitute of Animal Health, Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, ChinaHaemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.https://www.frontiersin.org/articles/10.3389/fvets.2021.756764/fullHaemophilus parasuisouter membrane vesiclespurificationproteome analysisinflammatory response |
| spellingShingle | Kunli Zhang Kunli Zhang Pinpin Chu Pinpin Chu Shuai Song Shuai Song Dongxia Yang Dongxia Yang Zhibiao Bian Zhibiao Bian Yan Li Yan Li Hongchao Gou Hongchao Gou Zhiyong Jiang Zhiyong Jiang Rujian Cai Rujian Cai Chunling Li Chunling Li Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis Frontiers in Veterinary Science Haemophilus parasuis outer membrane vesicles purification proteome analysis inflammatory response |
| title | Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis |
| title_full | Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis |
| title_fullStr | Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis |
| title_full_unstemmed | Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis |
| title_short | Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis |
| title_sort | proteome analysis of outer membrane vesicles from a highly virulent strain of haemophilus parasuis |
| topic | Haemophilus parasuis outer membrane vesicles purification proteome analysis inflammatory response |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.756764/full |
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