Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity
AbstractVibrio vulnificus an opportunistic human pathogen native to marine/estuarine environment, is one of the leading causes of death due to seafood consumption and exposure of wounds to seawater worldwide. The present study involves the whole genome sequence analysis of an environmental strain of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Veterinary Quarterly |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2023.2240389 |
| _version_ | 1797391740345253888 |
|---|---|
| author | Kattapuni Suresh Prithvisagar Pavan Gollapalli Caroline D’Souza Praveen Rai Iddya Karunasagar Indrani Karunasagar Krishna Kumar Ballamoole |
| author_facet | Kattapuni Suresh Prithvisagar Pavan Gollapalli Caroline D’Souza Praveen Rai Iddya Karunasagar Indrani Karunasagar Krishna Kumar Ballamoole |
| author_sort | Kattapuni Suresh Prithvisagar |
| collection | DOAJ |
| description | AbstractVibrio vulnificus an opportunistic human pathogen native to marine/estuarine environment, is one of the leading causes of death due to seafood consumption and exposure of wounds to seawater worldwide. The present study involves the whole genome sequence analysis of an environmental strain of V. vulnificus (clinical genotype) isolated from seafood along the Mangaluru coast of India. The sequenced genome data was subjected to in-silico analysis of phylogeny, virulence genes, antimicrobial resistance determinants, and secretary proteins using suitable bioinformatics tools. The sequenced isolate had an overall genome length of 4.8 Mb and GC content of 46% with 4400 coding DNA sequences. The sequenced strain belongs to a new sequence type (Multilocus sequence typing) and was also found to branch with a phylogenetic lineage that groups the most infectious strains of V. vulnificus. The seafood isolate had complete genes involved in conferring serum resistance yet showed limited serum resistance. The study identified several genes against the antibiotics that are commonly used in their treatment, highlighting the need for alternative treatments. Also, the secretory protein analysis revealed genes associated with major pathways like ABC transporters, two-component systems, quorum sensing, biofilm formation, cationic antimicrobial peptide (CAMP) resistance, and others that play a critical role in the pathogenesis of the V. vulnificus. To the best of our knowledge, this is the first report of a detailed analysis of the genomic information of a V. vulnificus isolated from the Indian subcontinent and provides evidence that raises public health concerns about the safety of seafood. |
| first_indexed | 2024-03-08T23:37:03Z |
| format | Article |
| id | doaj.art-ab1159c4400e4aaaac438d4934c74503 |
| institution | Directory Open Access Journal |
| issn | 0165-2176 1875-5941 |
| language | English |
| last_indexed | 2024-03-08T23:37:03Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj.art-ab1159c4400e4aaaac438d4934c745032023-12-14T06:25:09ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412023-12-0143111710.1080/01652176.2023.2240389Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicityKattapuni Suresh Prithvisagar0Pavan Gollapalli1Caroline D’Souza2Praveen Rai3Iddya Karunasagar4Indrani Karunasagar5Krishna Kumar Ballamoole6Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaCenter for Bioinformatics and Biostatistics, Nitte (Deemed to be University), Mangaluru, IndiaDepartment of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaDepartment of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaDepartment of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaDepartment of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaDepartment of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Nitte (Deemed to be University), Mangaluru, IndiaAbstractVibrio vulnificus an opportunistic human pathogen native to marine/estuarine environment, is one of the leading causes of death due to seafood consumption and exposure of wounds to seawater worldwide. The present study involves the whole genome sequence analysis of an environmental strain of V. vulnificus (clinical genotype) isolated from seafood along the Mangaluru coast of India. The sequenced genome data was subjected to in-silico analysis of phylogeny, virulence genes, antimicrobial resistance determinants, and secretary proteins using suitable bioinformatics tools. The sequenced isolate had an overall genome length of 4.8 Mb and GC content of 46% with 4400 coding DNA sequences. The sequenced strain belongs to a new sequence type (Multilocus sequence typing) and was also found to branch with a phylogenetic lineage that groups the most infectious strains of V. vulnificus. The seafood isolate had complete genes involved in conferring serum resistance yet showed limited serum resistance. The study identified several genes against the antibiotics that are commonly used in their treatment, highlighting the need for alternative treatments. Also, the secretory protein analysis revealed genes associated with major pathways like ABC transporters, two-component systems, quorum sensing, biofilm formation, cationic antimicrobial peptide (CAMP) resistance, and others that play a critical role in the pathogenesis of the V. vulnificus. To the best of our knowledge, this is the first report of a detailed analysis of the genomic information of a V. vulnificus isolated from the Indian subcontinent and provides evidence that raises public health concerns about the safety of seafood.https://www.tandfonline.com/doi/10.1080/01652176.2023.2240389Vibrio vulnificuswhole genome sequencepublic healthIndia |
| spellingShingle | Kattapuni Suresh Prithvisagar Pavan Gollapalli Caroline D’Souza Praveen Rai Iddya Karunasagar Indrani Karunasagar Krishna Kumar Ballamoole Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity Veterinary Quarterly Vibrio vulnificus whole genome sequence public health India |
| title | Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity |
| title_full | Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity |
| title_fullStr | Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity |
| title_full_unstemmed | Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity |
| title_short | Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity |
| title_sort | genome analysis of clinical genotype vibrio vulnificus isolated from seafood in mangaluru coast india provides insights into its pathogenicity |
| topic | Vibrio vulnificus whole genome sequence public health India |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2023.2240389 |
| work_keys_str_mv | AT kattapunisureshprithvisagar genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT pavangollapalli genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT carolinedsouza genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT praveenrai genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT iddyakarunasagar genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT indranikarunasagar genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity AT krishnakumarballamoole genomeanalysisofclinicalgenotypevibriovulnificusisolatedfromseafoodinmangalurucoastindiaprovidesinsightsintoitspathogenicity |