RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine
Korat chicken (KRC) is a slow-growing chicken bred in Thailand, whose meat exhibits a unique toughness. A previous study produced KRC breast meat containing high carnosine content through dietary supplementation with β-alanine or L-histidine; however, the KRC that were fed an L-histidine-supplemente...
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MDPI AG
2021-09-01
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| author | Satoshi Kubota Kasarat Promkhun Panpradub Sinpru Chanadda Suwanvichanee Wittawat Molee Amonrat Molee |
| author_facet | Satoshi Kubota Kasarat Promkhun Panpradub Sinpru Chanadda Suwanvichanee Wittawat Molee Amonrat Molee |
| author_sort | Satoshi Kubota |
| collection | DOAJ |
| description | Korat chicken (KRC) is a slow-growing chicken bred in Thailand, whose meat exhibits a unique toughness. A previous study produced KRC breast meat containing high carnosine content through dietary supplementation with β-alanine or L-histidine; however, the KRC that were fed an L-histidine-supplemented diet produced meat that was significantly more tender. Herein, we performed RNA-Seq to identify candidate genes involved in the regulation of carnosine content and meat toughness. Total RNA was isolated from five female KRC breast muscles in each treatment group that KRC fed diets without supplementation, supplemented with β-alanine or L-histidine. Compared to the non-supplemented group, we identified 118 and 198 differentially expressed genes (DEGs) in the β-alanine or L-histidine supplementation groups, respectively. Genes potentially related to meat tenderness—i.e., those regulating myosin, collagen, intramuscular fat, and calpain—were upregulated (<i>LOC107051274</i>, <i>ACSBG1</i>, and <i>CAPNS2</i>) and downregulated (<i>MYO7B</i>, <i>MYBPH</i>, <i>SERPINH1</i>, and <i>PGAM1</i>). However, carnosine synthase gene was not identified. Functional enrichment analysis identified pathways affected by dietary supplementation, including the insulin signaling pathway (β-alanine supplementation) and the insulin resistance and adipocytokine signaling pathways (L-histidine supplementation). The FoxO signaling pathway was identified as a regulatory network for both supplementation groups. The identified genes can be used as molecular markers of meat tenderness in slow-growing chickens. |
| first_indexed | 2024-03-10T07:58:20Z |
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| language | English |
| last_indexed | 2024-03-10T07:58:20Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-ab11f0df2e72453db7f0c02984604f582023-11-22T11:42:34ZengMDPI AGAnimals2076-26152021-09-01119259610.3390/ani11092596RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-HistidineSatoshi Kubota0Kasarat Promkhun1Panpradub Sinpru2Chanadda Suwanvichanee3Wittawat Molee4Amonrat Molee5School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandKorat chicken (KRC) is a slow-growing chicken bred in Thailand, whose meat exhibits a unique toughness. A previous study produced KRC breast meat containing high carnosine content through dietary supplementation with β-alanine or L-histidine; however, the KRC that were fed an L-histidine-supplemented diet produced meat that was significantly more tender. Herein, we performed RNA-Seq to identify candidate genes involved in the regulation of carnosine content and meat toughness. Total RNA was isolated from five female KRC breast muscles in each treatment group that KRC fed diets without supplementation, supplemented with β-alanine or L-histidine. Compared to the non-supplemented group, we identified 118 and 198 differentially expressed genes (DEGs) in the β-alanine or L-histidine supplementation groups, respectively. Genes potentially related to meat tenderness—i.e., those regulating myosin, collagen, intramuscular fat, and calpain—were upregulated (<i>LOC107051274</i>, <i>ACSBG1</i>, and <i>CAPNS2</i>) and downregulated (<i>MYO7B</i>, <i>MYBPH</i>, <i>SERPINH1</i>, and <i>PGAM1</i>). However, carnosine synthase gene was not identified. Functional enrichment analysis identified pathways affected by dietary supplementation, including the insulin signaling pathway (β-alanine supplementation) and the insulin resistance and adipocytokine signaling pathways (L-histidine supplementation). The FoxO signaling pathway was identified as a regulatory network for both supplementation groups. The identified genes can be used as molecular markers of meat tenderness in slow-growing chickens.https://www.mdpi.com/2076-2615/11/9/2596RNA-Seqslow-growing chickenbreast meatcarnosine contentmeat toughness |
| spellingShingle | Satoshi Kubota Kasarat Promkhun Panpradub Sinpru Chanadda Suwanvichanee Wittawat Molee Amonrat Molee RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine Animals RNA-Seq slow-growing chicken breast meat carnosine content meat toughness |
| title | RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine |
| title_full | RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine |
| title_fullStr | RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine |
| title_full_unstemmed | RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine |
| title_short | RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine |
| title_sort | rna profiles of the korat chicken breast muscle with increased carnosine content produced through dietary supplementation with β alanine or l histidine |
| topic | RNA-Seq slow-growing chicken breast meat carnosine content meat toughness |
| url | https://www.mdpi.com/2076-2615/11/9/2596 |
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