Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites
Objective: The effect of Artemisia herba-alba methanolic extract monotherapy and combination therapies on the in vitro growth of several Babesia and Theileria parasites in vitro and mice was investigated in this study. Materials and Methods: Fluorescence assay using SYBR Green I stain was used to e...
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| Format: | Article |
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Network for the Veterinarians of Bangladesh
2022-06-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=31667 |
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| author | Rasha Eltaysh Mohamed Abdo Rizk Shimaa Abd El-Salam El-Sayed Khaled Abouelnasr Abdelnaser Ahmed Abdallah Ikuo Igarashi |
| author_facet | Rasha Eltaysh Mohamed Abdo Rizk Shimaa Abd El-Salam El-Sayed Khaled Abouelnasr Abdelnaser Ahmed Abdallah Ikuo Igarashi |
| author_sort | Rasha Eltaysh |
| collection | DOAJ |
| description | Objective: The effect of Artemisia herba-alba methanolic extract monotherapy and combination therapies on the in vitro growth of several Babesia and Theileria parasites in vitro and mice was investigated in this study.
Materials and Methods: Fluorescence assay using SYBR Green I stain was used to evaluate the antibabesial efficacy inhibitory of A. herba-alba either in vitro or in vivo. Hematological parameters in the treated mice were analyzed using a Celltac MEK-6450 computerized hematology analyzer.
Results: Artemisia herba-alba reduced the growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi in vitro in a dose-dependent manner. The in vitro inhibitory impact of A. herba-alba on B. divergens and B. caballi cultures was amplified when combined with either diminazene aceturate (DA). In B. microti-infected mice, a combination therapy consisting of A. herba-alba and a low DA dose inhibited B. microti growth significantly (p < 0.05) better than treatment with 25 mg kg−1 DA.
Conclusions: These data show that A. herba-alba, when paired with a modest DA dose, could be a promising medicinal plant for babesiosis treatment. [J Adv Vet Anim Res 2022; 9(2.000): 267-274] |
| first_indexed | 2024-04-13T20:55:40Z |
| format | Article |
| id | doaj.art-ab1417e54ff64561af942f053df43826 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-13T20:55:40Z |
| publishDate | 2022-06-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-ab1417e54ff64561af942f053df438262022-12-22T02:30:20ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102022-06-019226727410.5455/javar.2022.i59231667Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasitesRasha Eltaysh0Mohamed Abdo Rizk1Shimaa Abd El-Salam El-Sayed2Khaled Abouelnasr3Abdelnaser Ahmed Abdallah4Ikuo Igarashi5National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. & Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. & Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. & Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.Objective: The effect of Artemisia herba-alba methanolic extract monotherapy and combination therapies on the in vitro growth of several Babesia and Theileria parasites in vitro and mice was investigated in this study. Materials and Methods: Fluorescence assay using SYBR Green I stain was used to evaluate the antibabesial efficacy inhibitory of A. herba-alba either in vitro or in vivo. Hematological parameters in the treated mice were analyzed using a Celltac MEK-6450 computerized hematology analyzer. Results: Artemisia herba-alba reduced the growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi in vitro in a dose-dependent manner. The in vitro inhibitory impact of A. herba-alba on B. divergens and B. caballi cultures was amplified when combined with either diminazene aceturate (DA). In B. microti-infected mice, a combination therapy consisting of A. herba-alba and a low DA dose inhibited B. microti growth significantly (p < 0.05) better than treatment with 25 mg kg−1 DA. Conclusions: These data show that A. herba-alba, when paired with a modest DA dose, could be a promising medicinal plant for babesiosis treatment. [J Adv Vet Anim Res 2022; 9(2.000): 267-274]http://www.ejmanager.com/fulltextpdf.php?mno=31667babesia; theileria; artemisia herbaalba; in vitro; in vivo; combination therapy |
| spellingShingle | Rasha Eltaysh Mohamed Abdo Rizk Shimaa Abd El-Salam El-Sayed Khaled Abouelnasr Abdelnaser Ahmed Abdallah Ikuo Igarashi Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites Journal of Advanced Veterinary and Animal Research babesia; theileria; artemisia herbaalba; in vitro; in vivo; combination therapy |
| title | Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites |
| title_full | Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites |
| title_fullStr | Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites |
| title_full_unstemmed | Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites |
| title_short | Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites |
| title_sort | evaluation of the in vitro and in vivo inhibitory effects of artemisia herba alba against the growth of piroplasm parasites |
| topic | babesia; theileria; artemisia herbaalba; in vitro; in vivo; combination therapy |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=31667 |
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