Isolation of new Klebsiella pneumoniae phage PSKP16
Background and Objectives: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3899 |
| _version_ | 1797902830781071360 |
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| author | Sara Sadeqi Slawomir Michniewski Farhad Nikkhahi Eleanor Jameson Seyed Mahmoud Amin Marashi |
| author_facet | Sara Sadeqi Slawomir Michniewski Farhad Nikkhahi Eleanor Jameson Seyed Mahmoud Amin Marashi |
| author_sort | Sara Sadeqi |
| collection | DOAJ |
| description |
Background and Objectives: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.
Materials and Methods: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.
Results: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.
Conclusion: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.
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| first_indexed | 2024-04-10T09:22:22Z |
| format | Article |
| id | doaj.art-ab14243db29d453e9a90164adfe35927 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-10T09:22:22Z |
| publishDate | 2023-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-ab14243db29d453e9a90164adfe359272023-02-20T08:49:12ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-02-0115110.18502/ijm.v15i1.11919Isolation of new Klebsiella pneumoniae phage PSKP16Sara Sadeqi0Slawomir Michniewski1Farhad Nikkhahi2Eleanor Jameson3Seyed Mahmoud Amin Marashi4Department of Medical Microbiology, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Life Sciences, University of Warwick, Coventry, United KingdomMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Life Sciences, University of Warwick, Coventry, United Kingdom; Department of Natural Sciences, Bangor University, Bangor, Gwynedd, United KingdomMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran Background and Objectives: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections. Materials and Methods: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology. Results: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1. Conclusion: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections. https://ijm.tums.ac.ir/index.php/ijm/article/view/3899Klebsiella pneumoniae;Bacteriophages;Drug resistance;Infection control;High throughput nucleotide sequencing |
| spellingShingle | Sara Sadeqi Slawomir Michniewski Farhad Nikkhahi Eleanor Jameson Seyed Mahmoud Amin Marashi Isolation of new Klebsiella pneumoniae phage PSKP16 Iranian Journal of Microbiology Klebsiella pneumoniae; Bacteriophages; Drug resistance; Infection control; High throughput nucleotide sequencing |
| title | Isolation of new Klebsiella pneumoniae phage PSKP16 |
| title_full | Isolation of new Klebsiella pneumoniae phage PSKP16 |
| title_fullStr | Isolation of new Klebsiella pneumoniae phage PSKP16 |
| title_full_unstemmed | Isolation of new Klebsiella pneumoniae phage PSKP16 |
| title_short | Isolation of new Klebsiella pneumoniae phage PSKP16 |
| title_sort | isolation of new klebsiella pneumoniae phage pskp16 |
| topic | Klebsiella pneumoniae; Bacteriophages; Drug resistance; Infection control; High throughput nucleotide sequencing |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3899 |
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