Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae)
In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of <i>Acianthera</i>, incompatible pollen tubes with anomalous morphology reach the ovar...
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MDPI AG
2020-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1758 |
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| author | Mariana Oliveira Duarte Denise Maria Trombert Oliveira Eduardo Leite Borba |
| author_facet | Mariana Oliveira Duarte Denise Maria Trombert Oliveira Eduardo Leite Borba |
| author_sort | Mariana Oliveira Duarte |
| collection | DOAJ |
| description | In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of <i>Acianthera</i>, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct self-incompatibility (SI) system is acting on the ovary of <i>A. johannensis</i>, which is a species with partial self-incompatibility, contrasting with a full SI species, <i>A. fabiobarrosii</i>. We analyzed the morphology and development of pollen tubes in the column, ovary, and fruit using light, epifluorescence, and transmission electron microscopy. Our results show that the main reaction site in <i>A. johannensis</i> is in the stylar canal inside the column, which was also recorded in <i>A. fabiobarrosii</i>. Morphological and cytological characteristics of the pollen tubes with obstructed growth in the column indicated a process of programmed cell death in these tubes, showing a possible GSI reaction. In addition, partially self-incompatible individuals of <i>A. johannensis</i> exhibit a second SI site in the ovary. We suggest that this self-incompatibility site in the ovary is only an extension of GSI that acts in the column, differing from the typical late-acting self-incompatibility system recorded in other plant groups. |
| first_indexed | 2024-03-10T14:08:01Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:08:01Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-ab1946483ffb4ea0b6b3d7a27b91920f2023-11-21T00:27:13ZengMDPI AGPlants2223-77472020-12-01912175810.3390/plants9121758Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae)Mariana Oliveira Duarte0Denise Maria Trombert Oliveira1Eduardo Leite Borba2Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte 31270-901, Minas Gerais, BrazilDepartamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte 31270-901, Minas Gerais, BrazilDepartamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, Pampulha, Belo Horizonte 31270-901, Minas Gerais, BrazilIn most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of <i>Acianthera</i>, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct self-incompatibility (SI) system is acting on the ovary of <i>A. johannensis</i>, which is a species with partial self-incompatibility, contrasting with a full SI species, <i>A. fabiobarrosii</i>. We analyzed the morphology and development of pollen tubes in the column, ovary, and fruit using light, epifluorescence, and transmission electron microscopy. Our results show that the main reaction site in <i>A. johannensis</i> is in the stylar canal inside the column, which was also recorded in <i>A. fabiobarrosii</i>. Morphological and cytological characteristics of the pollen tubes with obstructed growth in the column indicated a process of programmed cell death in these tubes, showing a possible GSI reaction. In addition, partially self-incompatible individuals of <i>A. johannensis</i> exhibit a second SI site in the ovary. We suggest that this self-incompatibility site in the ovary is only an extension of GSI that acts in the column, differing from the typical late-acting self-incompatibility system recorded in other plant groups.https://www.mdpi.com/2223-7747/9/12/1758gametophytic self-incompatibilitymating systemspollen tube ultrastructurepre-zygotic barriersprogrammed cell death |
| spellingShingle | Mariana Oliveira Duarte Denise Maria Trombert Oliveira Eduardo Leite Borba Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) Plants gametophytic self-incompatibility mating systems pollen tube ultrastructure pre-zygotic barriers programmed cell death |
| title | Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) |
| title_full | Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) |
| title_fullStr | Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) |
| title_full_unstemmed | Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) |
| title_short | Two Self-Incompatibility Sites Occur Simultaneously in the Same <i>Acianthera</i> Species (Orchidaceae, Pleurothallidinae) |
| title_sort | two self incompatibility sites occur simultaneously in the same i acianthera i species orchidaceae pleurothallidinae |
| topic | gametophytic self-incompatibility mating systems pollen tube ultrastructure pre-zygotic barriers programmed cell death |
| url | https://www.mdpi.com/2223-7747/9/12/1758 |
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