Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat
Abstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine corre...
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| Format: | Article |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78367-2 |
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| author | Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour |
| author_facet | Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour |
| author_sort | Md. Masudur Rahman |
| collection | DOAJ |
| description | Abstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine correlation between resistance phenotype and genotype. A total of 600 chicken meat swabs (divided equally between broiler and layer farms, n = 300 each) were collected and the isolates identified as E. coli (n = 381) were selected. Disc diffusion antimicrobial susceptibility assay showed resistance of these isolates to ampicillin, erythromycin, tetracycline, streptomycin, trimethoprim-sulfamethoxazole, chloramphenicol, and gentamicin. Polymerase chain reaction (PCR) identified several antibiotic resistance genes (ARGs) in our isolates. Among these ARGs, the prevalence of tetA (for tetracycline) was the highest (72.58%) in broiler chicken isolates, followed by sul1 (for sulfonamide; 44.16%), aadA1 (for streptomycin; 33.50%), ereA (for erythromycin; 27.41%), aac-3-IV (for gentamicin; 25.38%), and the two genes cmlA (24.87%) and catA1 (8.63%) for chloramphenicol. On the other hand, the respective prevalence in layer chicken isolates were 82.06%, 47.83%, 35.87%, 35.33%, 23.91%, 19.02%, and 5.43%. Furthermore, 49.23% of the isolates from broiler chicken were MDR, with the presence of multiple antibiotic resistance genes, including 3 (40.11%) and 4 (9.13%) genes. On the other hand, 51.09% of layer chicken E. coli isolates were MDR, with 3, 4 or 5 ARGs detected in 36.41%, 14.13%, and 0.54% of the isolates, respectively. We also found that 12.8% of broiler chicken E. coli isolates and 7.61% of layer chicken isolates carried genes coding for extended-spectrum SHV beta-lactamases. Lastly, we report the presence of the AmpC beta-lactamase producing gene (CITM) in 4.56% and 3.26% of broiler and layer chicken E. coli isolates, respectively. We found significant correlations between most of the antimicrobial resistant phenotypes and genotypes observed among the investigated E. coli isolates. Our findings highlight the need for the prudent use of antimicrobials in chickens to minimize the development of antibiotic-resistant bacterial strains. |
| first_indexed | 2024-12-18T05:16:58Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T05:16:58Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ab19a1318aaf428aae6e545409b85bc62022-12-21T21:19:45ZengNature PortfolioScientific Reports2045-23222020-12-0110111110.1038/s41598-020-78367-2Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meatMd. Masudur Rahman0Asmaul Husna1Hatem A. Elshabrawy2Jahangir Alam3Nurjahan Yasmin Runa4A. T. M. Badruzzaman5Nahid Arjuman Banu6Mohammad Al Mamun7Bashudeb Paul8Shobhan Das9Md. Mahfujur Rahman10A. T. M. Mahbub-E-Elahi11Ahmed S. Khairalla12Hossam M. Ashour13Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Molecular and Cellular Biology, College of Osteopathic Medicine, Sam Houston State UniversityAnimal Biotechnology Division, National Institute of BiotechnologyDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Anatomy and Histology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityDepartment of Microbiology and Immunology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural UniversityMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Integrative Biology, College of Arts and Sciences, University of South FloridaAbstract Antibiotic-resistant Escherichia coli (E. coli) are common in retail poultry products. In this study, we aimed to isolate and characterize multidrug resistant (MDR) E. coli in raw chicken meat samples collected from poultry shops in Sylhet division, Bangladesh, as well as to determine correlation between resistance phenotype and genotype. A total of 600 chicken meat swabs (divided equally between broiler and layer farms, n = 300 each) were collected and the isolates identified as E. coli (n = 381) were selected. Disc diffusion antimicrobial susceptibility assay showed resistance of these isolates to ampicillin, erythromycin, tetracycline, streptomycin, trimethoprim-sulfamethoxazole, chloramphenicol, and gentamicin. Polymerase chain reaction (PCR) identified several antibiotic resistance genes (ARGs) in our isolates. Among these ARGs, the prevalence of tetA (for tetracycline) was the highest (72.58%) in broiler chicken isolates, followed by sul1 (for sulfonamide; 44.16%), aadA1 (for streptomycin; 33.50%), ereA (for erythromycin; 27.41%), aac-3-IV (for gentamicin; 25.38%), and the two genes cmlA (24.87%) and catA1 (8.63%) for chloramphenicol. On the other hand, the respective prevalence in layer chicken isolates were 82.06%, 47.83%, 35.87%, 35.33%, 23.91%, 19.02%, and 5.43%. Furthermore, 49.23% of the isolates from broiler chicken were MDR, with the presence of multiple antibiotic resistance genes, including 3 (40.11%) and 4 (9.13%) genes. On the other hand, 51.09% of layer chicken E. coli isolates were MDR, with 3, 4 or 5 ARGs detected in 36.41%, 14.13%, and 0.54% of the isolates, respectively. We also found that 12.8% of broiler chicken E. coli isolates and 7.61% of layer chicken isolates carried genes coding for extended-spectrum SHV beta-lactamases. Lastly, we report the presence of the AmpC beta-lactamase producing gene (CITM) in 4.56% and 3.26% of broiler and layer chicken E. coli isolates, respectively. We found significant correlations between most of the antimicrobial resistant phenotypes and genotypes observed among the investigated E. coli isolates. Our findings highlight the need for the prudent use of antimicrobials in chickens to minimize the development of antibiotic-resistant bacterial strains.https://doi.org/10.1038/s41598-020-78367-2 |
| spellingShingle | Md. Masudur Rahman Asmaul Husna Hatem A. Elshabrawy Jahangir Alam Nurjahan Yasmin Runa A. T. M. Badruzzaman Nahid Arjuman Banu Mohammad Al Mamun Bashudeb Paul Shobhan Das Md. Mahfujur Rahman A. T. M. Mahbub-E-Elahi Ahmed S. Khairalla Hossam M. Ashour Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat Scientific Reports |
| title | Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat |
| title_full | Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat |
| title_fullStr | Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat |
| title_full_unstemmed | Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat |
| title_short | Isolation and molecular characterization of multidrug-resistant Escherichia coli from chicken meat |
| title_sort | isolation and molecular characterization of multidrug resistant escherichia coli from chicken meat |
| url | https://doi.org/10.1038/s41598-020-78367-2 |
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