Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-01-01
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| Series: | Mycobiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/12298093.2018.1454004 |
| _version_ | 1819146486527557632 |
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| author | Fang-Jie Yao Li-Xin Lu Peng Wang Ming Fang You-Min Zhang Ying Chen Wei-Tong Zhang Xiang-Hui Kong Jia Lu Yoichi Honda |
| author_facet | Fang-Jie Yao Li-Xin Lu Peng Wang Ming Fang You-Min Zhang Ying Chen Wei-Tong Zhang Xiang-Hui Kong Jia Lu Yoichi Honda |
| author_sort | Fang-Jie Yao |
| collection | DOAJ |
| description | The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae. |
| first_indexed | 2024-12-22T13:14:41Z |
| format | Article |
| id | doaj.art-ab1a7140818242a585ac0570912dc63a |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-22T13:14:41Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-ab1a7140818242a585ac0570912dc63a2022-12-21T18:24:39ZengTaylor & Francis GroupMycobiology1229-80932092-93232018-01-01461727810.1080/12298093.2018.14540041454004Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judaeFang-Jie Yao0Li-Xin Lu1Peng Wang2Ming Fang3You-Min Zhang4Ying Chen5Wei-Tong Zhang6Xiang-Hui Kong7Jia Lu8Yoichi Honda9Jilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityJilin Agricultural UniversityKyoto UniversityThe fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.http://dx.doi.org/10.1080/12298093.2018.1454004Auricularia auricula-judaefruiting body patternsequence-related amplified polymorphismsequence characterized amplified region |
| spellingShingle | Fang-Jie Yao Li-Xin Lu Peng Wang Ming Fang You-Min Zhang Ying Chen Wei-Tong Zhang Xiang-Hui Kong Jia Lu Yoichi Honda Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae Mycobiology Auricularia auricula-judae fruiting body pattern sequence-related amplified polymorphism sequence characterized amplified region |
| title | Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae |
| title_full | Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae |
| title_fullStr | Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae |
| title_full_unstemmed | Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae |
| title_short | Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae |
| title_sort | development of a molecular marker for fruiting body pattern in auricularia auricula judae |
| topic | Auricularia auricula-judae fruiting body pattern sequence-related amplified polymorphism sequence characterized amplified region |
| url | http://dx.doi.org/10.1080/12298093.2018.1454004 |
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