Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment
Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old <i>Arabidopsis thaliana</i> (L.) Heynh. seedling...
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MDPI AG
2022-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/11/1822 |
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| author | Alexandra Waskow Anthony Guihur Alan Howling Ivo Furno |
| author_facet | Alexandra Waskow Anthony Guihur Alan Howling Ivo Furno |
| author_sort | Alexandra Waskow |
| collection | DOAJ |
| description | Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old <i>Arabidopsis thaliana</i> (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response. |
| first_indexed | 2024-03-09T18:55:03Z |
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| id | doaj.art-ab1c23e8bd4c4be0a710704fb879ec03 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T18:55:03Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-ab1c23e8bd4c4be0a710704fb879ec032023-11-24T05:31:30ZengMDPI AGLife2075-17292022-11-011211182210.3390/life12111822Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed TreatmentAlexandra Waskow0Anthony Guihur1Alan Howling2Ivo Furno3Swiss Plasma Center (SPC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, SwitzerlandDepartment of Plant Molecular Biology, Faculty of Biology and Medicine, University of Lausanne (UNIL), CH-1015 Lausanne, SwitzerlandSwiss Plasma Center (SPC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, SwitzerlandSwiss Plasma Center (SPC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, SwitzerlandNon-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old <i>Arabidopsis thaliana</i> (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response.https://www.mdpi.com/2075-1729/12/11/1822non-thermal plasmaplant defenseglucosinolatesnitrilesRNA sequencing<i>Arabidopsis thaliana</i> |
| spellingShingle | Alexandra Waskow Anthony Guihur Alan Howling Ivo Furno Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment Life non-thermal plasma plant defense glucosinolates nitriles RNA sequencing <i>Arabidopsis thaliana</i> |
| title | Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment |
| title_full | Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment |
| title_fullStr | Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment |
| title_full_unstemmed | Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment |
| title_short | Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of <i>Arabidopsis thaliana</i> Seedlings after Non-Thermal Plasma-Seed Treatment |
| title_sort | catabolism of glucosinolates into nitriles revealed by rna sequencing of i arabidopsis thaliana i seedlings after non thermal plasma seed treatment |
| topic | non-thermal plasma plant defense glucosinolates nitriles RNA sequencing <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/2075-1729/12/11/1822 |
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