Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy
The progression of periodontitis depends on interactions between the periodontal pathogens and the host immune cytokines, including interleukin (IL)-1β and IL-18. Production of IL-1β is regulated by NOD-like receptors family pyrin domain containing 3 (NLRP3). This study aimed to evaluate the effect...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-12-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2821 |
| _version_ | 1818772625241931776 |
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| author | Shahrzad Shahbeik Ferial Taleghani Mandana Sattari Mahyar Mahvash Mohammadi Mohamadreza Moravej |
| author_facet | Shahrzad Shahbeik Ferial Taleghani Mandana Sattari Mahyar Mahvash Mohammadi Mohamadreza Moravej |
| author_sort | Shahrzad Shahbeik |
| collection | DOAJ |
| description | The progression of periodontitis depends on interactions between the periodontal pathogens and the host immune cytokines, including interleukin (IL)-1β and IL-18. Production of IL-1β is regulated by NOD-like receptors family pyrin domain containing 3 (NLRP3). This study aimed to evaluate the effect of periodontal treatment on the concentrations of IL-18 and NLRP3 in patients with chronic periodontitis.
In this experimental study, 18 patients with chronic periodontitis and a mean age of 46.2±8.95 years, were included. The gingival crevicular fluid (GCF) was collected at the beginning of the study, 4 weeks after non-surgical (phase I), and 4 weeks after surgical periodontal treatment. The levels of NLRP3 and IL-18 were measured; using an enzyme-linked immunosorbent assay. Pearson correlation test was used to analyze the concentration of NLRP3 and IL-18 before and after the treatments with CAL and PD.
There was a significant association between the level of NLRP3 and the mean values of PD and CAL before treatment. After each treatment phase, a significant decrease was observed in the NLRP3 level. There was no significant relationship between IL-18 and clinical parameters before and after periodontal treatments.
Given the possible association between the level of NLRP3 and clinical parameters, we suggest it as a possible indicator of inflammation in chronic periodontitis and an index for evaluating the treatment outcome. |
| first_indexed | 2024-12-18T10:12:20Z |
| format | Article |
| id | doaj.art-ab1d999b3e9040b78ca017a4e7bbdd68 |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-18T10:12:20Z |
| publishDate | 2021-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-ab1d999b3e9040b78ca017a4e7bbdd682022-12-21T21:11:24ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492021-12-0120610.18502/ijaai.v20i6.8028Evaluation of NLRP3 and IL-18 Levels after Periodontal TherapyShahrzad Shahbeik0Ferial Taleghani1Mandana Sattari2Mahyar Mahvash Mohammadi3Mohamadreza Moravej4Department of Periodontology, Faculty of Dental, Alborz University, Karaj, IranDepartment of Periodontics, Faculty of Dentistry, Shahed University, Tehran, IranDepartment of Immunology, Shahid Beheshti University of Medical Sciences, Tehran, IranOrthodontist, Graduated of Dental Branch of Islamic Azad University, Tehran, IranFaculty of Dentistry, Alborz University, Karaj, IranThe progression of periodontitis depends on interactions between the periodontal pathogens and the host immune cytokines, including interleukin (IL)-1β and IL-18. Production of IL-1β is regulated by NOD-like receptors family pyrin domain containing 3 (NLRP3). This study aimed to evaluate the effect of periodontal treatment on the concentrations of IL-18 and NLRP3 in patients with chronic periodontitis. In this experimental study, 18 patients with chronic periodontitis and a mean age of 46.2±8.95 years, were included. The gingival crevicular fluid (GCF) was collected at the beginning of the study, 4 weeks after non-surgical (phase I), and 4 weeks after surgical periodontal treatment. The levels of NLRP3 and IL-18 were measured; using an enzyme-linked immunosorbent assay. Pearson correlation test was used to analyze the concentration of NLRP3 and IL-18 before and after the treatments with CAL and PD. There was a significant association between the level of NLRP3 and the mean values of PD and CAL before treatment. After each treatment phase, a significant decrease was observed in the NLRP3 level. There was no significant relationship between IL-18 and clinical parameters before and after periodontal treatments. Given the possible association between the level of NLRP3 and clinical parameters, we suggest it as a possible indicator of inflammation in chronic periodontitis and an index for evaluating the treatment outcome.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2821Chronic periodontitisInterleukin-18NLRP3 protein |
| spellingShingle | Shahrzad Shahbeik Ferial Taleghani Mandana Sattari Mahyar Mahvash Mohammadi Mohamadreza Moravej Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy Iranian Journal of Allergy, Asthma and Immunology Chronic periodontitis Interleukin-18 NLRP3 protein |
| title | Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy |
| title_full | Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy |
| title_fullStr | Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy |
| title_full_unstemmed | Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy |
| title_short | Evaluation of NLRP3 and IL-18 Levels after Periodontal Therapy |
| title_sort | evaluation of nlrp3 and il 18 levels after periodontal therapy |
| topic | Chronic periodontitis Interleukin-18 NLRP3 protein |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2821 |
| work_keys_str_mv | AT shahrzadshahbeik evaluationofnlrp3andil18levelsafterperiodontaltherapy AT ferialtaleghani evaluationofnlrp3andil18levelsafterperiodontaltherapy AT mandanasattari evaluationofnlrp3andil18levelsafterperiodontaltherapy AT mahyarmahvashmohammadi evaluationofnlrp3andil18levelsafterperiodontaltherapy AT mohamadrezamoravej evaluationofnlrp3andil18levelsafterperiodontaltherapy |