An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.)
Along with being important pigments that determining the flower color in many plants, anthocyanins also perform crucial functions that attract pollinators and reduce abiotic stresses. Purple and white are two different colors of radish petals. In this study, two cDNA libraries constructed with purpl...
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| Format: | Article |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.643579/full |
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| author | Dongming Liu Dongming Liu Xiaochun Wei Dongling Sun Shuangjuan Yang Henan Su Zhiyong Wang Yanyan Zhao Lin Li Jinfang Liang Luming Yang Xiaowei Zhang Yuxiang Yuan |
| author_facet | Dongming Liu Dongming Liu Xiaochun Wei Dongling Sun Shuangjuan Yang Henan Su Zhiyong Wang Yanyan Zhao Lin Li Jinfang Liang Luming Yang Xiaowei Zhang Yuxiang Yuan |
| author_sort | Dongming Liu |
| collection | DOAJ |
| description | Along with being important pigments that determining the flower color in many plants, anthocyanins also perform crucial functions that attract pollinators and reduce abiotic stresses. Purple and white are two different colors of radish petals. In this study, two cDNA libraries constructed with purple and white petal plants were sequenced for transcriptome profiling. Transcriptome results implied that the expression level of the genes participating in the anthocyanin biosynthetic pathway was commonly higher in the purple petals than that in the white petals. In particular, two genes, F3′H and DFR, had a significantly higher expression pattern in the purple petals, suggesting the important roles these genes playing in radish petal coloration. BSA-seq aided-Next Generation Sequencing of two DNA pools revealed that the radish purple petal gene (RsPP) was located on chromosome 7. With additional genotyping of 617 F2 population plants, the RsPP was further confined within a region of 93.23 kb. Transcriptome and Sanger sequencing analysis further helped identify the target gene, Rs392880. Rs392880 is a homologous gene to F3′H, a key gene in the anthocyanin biosynthetic pathway. These results will aid in elucidating the molecular mechanism of plant petal coloration and developing strategies to modify flower color through genetic transformation. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T17:54:23Z |
| publishDate | 2021-06-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-ab1e807ebf884b93acab8e502f0d58ba2022-12-21T22:22:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.643579643579An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.)Dongming Liu0Dongming Liu1Xiaochun Wei2Dongling Sun3Shuangjuan Yang4Henan Su5Zhiyong Wang6Yanyan Zhao7Lin Li8Jinfang Liang9Luming Yang10Xiaowei Zhang11Yuxiang Yuan12Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Horticulture, Henan Agricultural University, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Horticulture, Henan Agricultural University, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Horticulture, Henan Agricultural University, Zhengzhou, ChinaCollege of Horticulture, Henan Agricultural University, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaAlong with being important pigments that determining the flower color in many plants, anthocyanins also perform crucial functions that attract pollinators and reduce abiotic stresses. Purple and white are two different colors of radish petals. In this study, two cDNA libraries constructed with purple and white petal plants were sequenced for transcriptome profiling. Transcriptome results implied that the expression level of the genes participating in the anthocyanin biosynthetic pathway was commonly higher in the purple petals than that in the white petals. In particular, two genes, F3′H and DFR, had a significantly higher expression pattern in the purple petals, suggesting the important roles these genes playing in radish petal coloration. BSA-seq aided-Next Generation Sequencing of two DNA pools revealed that the radish purple petal gene (RsPP) was located on chromosome 7. With additional genotyping of 617 F2 population plants, the RsPP was further confined within a region of 93.23 kb. Transcriptome and Sanger sequencing analysis further helped identify the target gene, Rs392880. Rs392880 is a homologous gene to F3′H, a key gene in the anthocyanin biosynthetic pathway. These results will aid in elucidating the molecular mechanism of plant petal coloration and developing strategies to modify flower color through genetic transformation.https://www.frontiersin.org/articles/10.3389/fpls.2021.643579/fullradishtranscriptomeanthocyaninsgene mappingpetal color |
| spellingShingle | Dongming Liu Dongming Liu Xiaochun Wei Dongling Sun Shuangjuan Yang Henan Su Zhiyong Wang Yanyan Zhao Lin Li Jinfang Liang Luming Yang Xiaowei Zhang Yuxiang Yuan An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) Frontiers in Plant Science radish transcriptome anthocyanins gene mapping petal color |
| title | An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) |
| title_full | An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) |
| title_fullStr | An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) |
| title_full_unstemmed | An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) |
| title_short | An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.) |
| title_sort | snp mutation of gene rspp converts petal color from purple to white in radish raphanus sativus l |
| topic | radish transcriptome anthocyanins gene mapping petal color |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.643579/full |
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