Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells
Despite the ongoing clinical trials and the introduction of novel treatments over the past few decades, ovarian cancer remains one of the most fatal malignancies in women worldwide. Platinum- and paclitaxel-based chemotherapy is effective in treating the majority of patients with ovarian cancer. How...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/9/469 |
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| author | Chia-Yi Chan Yi-Chun Ni Hieu Duc Nguyen Yung-Fu Wu Kuen-Haur Lee |
| author_facet | Chia-Yi Chan Yi-Chun Ni Hieu Duc Nguyen Yung-Fu Wu Kuen-Haur Lee |
| author_sort | Chia-Yi Chan |
| collection | DOAJ |
| description | Despite the ongoing clinical trials and the introduction of novel treatments over the past few decades, ovarian cancer remains one of the most fatal malignancies in women worldwide. Platinum- and paclitaxel-based chemotherapy is effective in treating the majority of patients with ovarian cancer. However, more than 70% of patients experience recurrence and eventually develop chemoresistance. To improve clinical outcomes in patients with ovarian cancer, novel technologies must be developed for identifying molecular alterations following drug-based treatment of ovarian cancer. Recently, extracellular vesicles (EVs) have gained prominence as the mediators of tumor progression. In this study, we used mass spectrometry to identify the changes in EV protein signatures due to different chemotherapeutic agents used for treating ovarian cancer. By examining these alterations, we identified the specific protein induction patterns of cisplatin alone, paclitaxel alone, and a combination of cisplatin and paclitaxel. Specifically, we found that drug sensitivity was correlated with the expression levels of ANXA5, CD81, and RAB5C in patients receiving cisplatin with paclitaxel. Our findings suggest that chemotherapy-induced changes in EV protein signatures are crucial for the progression of ovarian cancer. |
| first_indexed | 2024-03-10T22:55:30Z |
| format | Article |
| id | doaj.art-ab23dc12c02e4a799b69aefcb4fe0daa |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T22:55:30Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-ab23dc12c02e4a799b69aefcb4fe0daa2023-11-19T10:05:00ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-09-014597417743110.3390/cimb45090469Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer CellsChia-Yi Chan0Yi-Chun Ni1Hieu Duc Nguyen2Yung-Fu Wu3Kuen-Haur Lee4Department of Nursing, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanGraduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanPhD Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanDepartment of Medical Research, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanGraduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanDespite the ongoing clinical trials and the introduction of novel treatments over the past few decades, ovarian cancer remains one of the most fatal malignancies in women worldwide. Platinum- and paclitaxel-based chemotherapy is effective in treating the majority of patients with ovarian cancer. However, more than 70% of patients experience recurrence and eventually develop chemoresistance. To improve clinical outcomes in patients with ovarian cancer, novel technologies must be developed for identifying molecular alterations following drug-based treatment of ovarian cancer. Recently, extracellular vesicles (EVs) have gained prominence as the mediators of tumor progression. In this study, we used mass spectrometry to identify the changes in EV protein signatures due to different chemotherapeutic agents used for treating ovarian cancer. By examining these alterations, we identified the specific protein induction patterns of cisplatin alone, paclitaxel alone, and a combination of cisplatin and paclitaxel. Specifically, we found that drug sensitivity was correlated with the expression levels of ANXA5, CD81, and RAB5C in patients receiving cisplatin with paclitaxel. Our findings suggest that chemotherapy-induced changes in EV protein signatures are crucial for the progression of ovarian cancer.https://www.mdpi.com/1467-3045/45/9/469ovarian cancerextracellular vesiclechemoresistanceprotein signature |
| spellingShingle | Chia-Yi Chan Yi-Chun Ni Hieu Duc Nguyen Yung-Fu Wu Kuen-Haur Lee Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells Current Issues in Molecular Biology ovarian cancer extracellular vesicle chemoresistance protein signature |
| title | Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells |
| title_full | Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells |
| title_fullStr | Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells |
| title_full_unstemmed | Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells |
| title_short | Identification of Potential Protein Targets in Extracellular Vesicles Isolated from Chemotherapy-Treated Ovarian Cancer Cells |
| title_sort | identification of potential protein targets in extracellular vesicles isolated from chemotherapy treated ovarian cancer cells |
| topic | ovarian cancer extracellular vesicle chemoresistance protein signature |
| url | https://www.mdpi.com/1467-3045/45/9/469 |
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