Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand?
Background: Plasmodium falciparum (P.f.)has developed resistance to most of the drugs that are being used currently. The continued search for a new drug is required to successfully eliminate the parasite. A pathway that is unique to the parasite is an ideal target to inhibit the growth of the parasi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031321001901 |
| _version_ | 1819290671688712192 |
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| author | Vikky Awasthi Yash Gupta Rubika Chauhan Prakasha Kempaiah Jyoti Das |
| author_facet | Vikky Awasthi Yash Gupta Rubika Chauhan Prakasha Kempaiah Jyoti Das |
| author_sort | Vikky Awasthi |
| collection | DOAJ |
| description | Background: Plasmodium falciparum (P.f.)has developed resistance to most of the drugs that are being used currently. The continued search for a new drug is required to successfully eliminate the parasite. A pathway that is unique to the parasite is an ideal target to inhibit the growth of the parasite without affecting the host metabolism. Purpose: In this study, Zingiber officinale Roscoe (Zingiberaceae)(Z.o.) extract, used ethnobotanically as a potent antimicrobial, was found to inhibit P.f. in vitro. Methods: To find the active compounds that may target the essential proteins of the P.f., the known secondary metabolite constituents of Z.o. rhizome were subjected to a preliminary in-silico target search. Chorismate synthase (CS) came up as a hit with a high binding score through multiple screening strategies. The antimalarial activity of the Z.o. extracts were tested with CS downstream metabolic products i.e. aromatic amino acids. The antimalarial targetablity of CS was confirmed with the phylogenetic conservancy, protein modeling, validation and subjected to High throughput virtual screening (HTVS) with a complete secondary metabolite library of Z.o. Among the top hits, the second hit 6-paradol (1-(4‑hydroxy-3-methoxyphenyl)decan-3-one) was available as a nature-identical synthetic compound. Compound 6-paradol was further analyzed for antimalarial and cytotoxic profiles and subjected to the same CS downstream product supplementation rescue assay. Results: 6-paradol is a highly potent antimalarial and a major component of Z.o. secondary metabolite pool. Though it is predicted to bind to the active site of chorismate synthase along with other metabolites of Z.o., an enzyme, part of the shikimate pathway involved in the aromatic amino acid synthesis. Parasite growth was rescued upon supplementation of aromatic amino acids along with crude Z.o. extract in parasite culture and a shift in IC50 of > 2 folds was observed, confirming the CS as the primary target of Z.o. crude extract. However, the rescue was inconsequential when we assayed with pure 6-paradol. Conclusion: This approach exploits natural compounds with high specificity and potency to kill pathogens developed due to eons of co-evolution with related pathogens. We report a novel potent antimalarial 6-paradol with nano-molar range IC50 already approved by FDA for human consumption as a nature-identical flavoring agent with immediate repurposing potential as an anti-malarial. However, CS rescue assay failure point to a different mechanism of action by active compounds and highlights Z.o. as a treasure trove of more potent anti-malarial which need further exploration. |
| first_indexed | 2024-12-24T03:26:27Z |
| format | Article |
| id | doaj.art-ab2667c2d03c450aa9867b0a74fd67f0 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-12-24T03:26:27Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
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| spelling | doaj.art-ab2667c2d03c450aa9867b0a74fd67f02022-12-21T17:17:20ZengElsevierPhytomedicine Plus2667-03132022-02-0121100208Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand?Vikky Awasthi0Yash Gupta1Rubika Chauhan2Prakasha Kempaiah3Jyoti Das4Parasite-Host Biology, National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, 110077, IndiaInfectious Diseases Division, Mayo Clinic, Jacksonville, Florida, USAParasite-Host Biology, National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, 110077, IndiaInfectious Diseases Division, Mayo Clinic, Jacksonville, Florida, USA; Corresponding authorParasite-Host Biology, National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, 110077, India; Corresponding authorBackground: Plasmodium falciparum (P.f.)has developed resistance to most of the drugs that are being used currently. The continued search for a new drug is required to successfully eliminate the parasite. A pathway that is unique to the parasite is an ideal target to inhibit the growth of the parasite without affecting the host metabolism. Purpose: In this study, Zingiber officinale Roscoe (Zingiberaceae)(Z.o.) extract, used ethnobotanically as a potent antimicrobial, was found to inhibit P.f. in vitro. Methods: To find the active compounds that may target the essential proteins of the P.f., the known secondary metabolite constituents of Z.o. rhizome were subjected to a preliminary in-silico target search. Chorismate synthase (CS) came up as a hit with a high binding score through multiple screening strategies. The antimalarial activity of the Z.o. extracts were tested with CS downstream metabolic products i.e. aromatic amino acids. The antimalarial targetablity of CS was confirmed with the phylogenetic conservancy, protein modeling, validation and subjected to High throughput virtual screening (HTVS) with a complete secondary metabolite library of Z.o. Among the top hits, the second hit 6-paradol (1-(4‑hydroxy-3-methoxyphenyl)decan-3-one) was available as a nature-identical synthetic compound. Compound 6-paradol was further analyzed for antimalarial and cytotoxic profiles and subjected to the same CS downstream product supplementation rescue assay. Results: 6-paradol is a highly potent antimalarial and a major component of Z.o. secondary metabolite pool. Though it is predicted to bind to the active site of chorismate synthase along with other metabolites of Z.o., an enzyme, part of the shikimate pathway involved in the aromatic amino acid synthesis. Parasite growth was rescued upon supplementation of aromatic amino acids along with crude Z.o. extract in parasite culture and a shift in IC50 of > 2 folds was observed, confirming the CS as the primary target of Z.o. crude extract. However, the rescue was inconsequential when we assayed with pure 6-paradol. Conclusion: This approach exploits natural compounds with high specificity and potency to kill pathogens developed due to eons of co-evolution with related pathogens. We report a novel potent antimalarial 6-paradol with nano-molar range IC50 already approved by FDA for human consumption as a nature-identical flavoring agent with immediate repurposing potential as an anti-malarial. However, CS rescue assay failure point to a different mechanism of action by active compounds and highlights Z.o. as a treasure trove of more potent anti-malarial which need further exploration.http://www.sciencedirect.com/science/article/pii/S2667031321001901MalariaPlasmodiumGingerChorismate synthase |
| spellingShingle | Vikky Awasthi Yash Gupta Rubika Chauhan Prakasha Kempaiah Jyoti Das Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? Phytomedicine Plus Malaria Plasmodium Ginger Chorismate synthase |
| title | Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? |
| title_full | Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? |
| title_fullStr | Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? |
| title_full_unstemmed | Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? |
| title_short | Growth inhibition of plasmodium falciparum by Nano-molar concentrations of 1-(4‑hydroxy-3-methoxyphenyl) decan-3-one (6-paradol); is a cure at hand? |
| title_sort | growth inhibition of plasmodium falciparum by nano molar concentrations of 1 4 hydroxy 3 methoxyphenyl decan 3 one 6 paradol is a cure at hand |
| topic | Malaria Plasmodium Ginger Chorismate synthase |
| url | http://www.sciencedirect.com/science/article/pii/S2667031321001901 |
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