Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast
<i>Petiveria alliacea</i> L. is a plant used in traditional medicine harboring pharmacological properties with anti-inflammatory, antinociceptive, hypoglycemiant and anesthetic activities. This study assessed the potential cytotoxic, genotoxic and mutagenic effects of ethanolic extract o...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/23/3263 |
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| author | Bruna B. F. Cal Luana B. N. Araújo Brenno M. Nunes Claudia R. da Silva Marcia B. N. Oliveira Bianka O. Soares Alvaro A. C. Leitão Marcelo de Pádula Debora Nascimento Douglas S. A. Chaves Rachel F. Gagliardi Flavio J. S. Dantas |
| author_facet | Bruna B. F. Cal Luana B. N. Araújo Brenno M. Nunes Claudia R. da Silva Marcia B. N. Oliveira Bianka O. Soares Alvaro A. C. Leitão Marcelo de Pádula Debora Nascimento Douglas S. A. Chaves Rachel F. Gagliardi Flavio J. S. Dantas |
| author_sort | Bruna B. F. Cal |
| collection | DOAJ |
| description | <i>Petiveria alliacea</i> L. is a plant used in traditional medicine harboring pharmacological properties with anti-inflammatory, antinociceptive, hypoglycemiant and anesthetic activities. This study assessed the potential cytotoxic, genotoxic and mutagenic effects of ethanolic extract of <i>P. alliacea</i> on <i>Saccharomyces cerevisiae</i> strains. <i>S. cerevisiae</i> FF18733 (wild type) and CD138 (<i>ogg1</i>) strains were exposed to fractioned ethanolic extracts of <i>P. alliacea</i> in different concentrations. Three experimental assays were performed: cellular inactivation, mutagenesis (canavanine resistance system) and loss of mitochondrial function (petites colonies). The chemical analyses revealed a rich extract with phenolic compounds such as protocatechuic acid, cinnamic and catechin epicatechin. A decreased cell viability in wild-type and <i>ogg1</i> strains was demonstrated. All fractions of the extract exerted a mutagenic effect on the <i>ogg1</i> strain. Only ethyl acetate and n-butanol fractions increased the rate of petites colonies in the <i>ogg1</i> strain, but not in the wild-type strain. The results indicate that fractions of mid-polarity of the ethanolic extract, at the studied concentrations, can induce mutagenicity mediated by oxidative lesions in the mitochondrial and genomic genomes of the ogg1-deficient <i>S. cerevisiae</i> strain. These findings indicate that the lesions caused by the fractions of <i>P. alliacea</i> ethanolic extract can be mediated by reactive oxygen species and can reach multiple molecular targets to exert their toxicity. |
| first_indexed | 2024-03-09T17:35:55Z |
| format | Article |
| id | doaj.art-ab27b9dc8ad84a80a12634094c4ee0e0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T17:35:55Z |
| publishDate | 2022-11-01 |
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| series | Plants |
| spelling | doaj.art-ab27b9dc8ad84a80a12634094c4ee0e02023-11-24T11:55:02ZengMDPI AGPlants2223-77472022-11-011123326310.3390/plants11233263Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on YeastBruna B. F. Cal0Luana B. N. Araújo1Brenno M. Nunes2Claudia R. da Silva3Marcia B. N. Oliveira4Bianka O. Soares5Alvaro A. C. Leitão6Marcelo de Pádula7Debora Nascimento8Douglas S. A. Chaves9Rachel F. Gagliardi10Flavio J. S. Dantas11Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, BrazilDepartamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, BrazilDepartamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, BrazilDepartamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, BrazilDepartamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, BrazilNúcleo de Biotecnologia Vegetal, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20550-013, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, BrazilLaboratório de Microbiologia e Avaliação Genotóxica (LAMIAG), Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, BrazilLaboratório de Química de Bioativos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro 23897-000, BrazilLaboratório de Química de Bioativos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro 23897-000, BrazilNúcleo de Biotecnologia Vegetal, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20550-013, BrazilDepartamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20551-030, Brazil<i>Petiveria alliacea</i> L. is a plant used in traditional medicine harboring pharmacological properties with anti-inflammatory, antinociceptive, hypoglycemiant and anesthetic activities. This study assessed the potential cytotoxic, genotoxic and mutagenic effects of ethanolic extract of <i>P. alliacea</i> on <i>Saccharomyces cerevisiae</i> strains. <i>S. cerevisiae</i> FF18733 (wild type) and CD138 (<i>ogg1</i>) strains were exposed to fractioned ethanolic extracts of <i>P. alliacea</i> in different concentrations. Three experimental assays were performed: cellular inactivation, mutagenesis (canavanine resistance system) and loss of mitochondrial function (petites colonies). The chemical analyses revealed a rich extract with phenolic compounds such as protocatechuic acid, cinnamic and catechin epicatechin. A decreased cell viability in wild-type and <i>ogg1</i> strains was demonstrated. All fractions of the extract exerted a mutagenic effect on the <i>ogg1</i> strain. Only ethyl acetate and n-butanol fractions increased the rate of petites colonies in the <i>ogg1</i> strain, but not in the wild-type strain. The results indicate that fractions of mid-polarity of the ethanolic extract, at the studied concentrations, can induce mutagenicity mediated by oxidative lesions in the mitochondrial and genomic genomes of the ogg1-deficient <i>S. cerevisiae</i> strain. These findings indicate that the lesions caused by the fractions of <i>P. alliacea</i> ethanolic extract can be mediated by reactive oxygen species and can reach multiple molecular targets to exert their toxicity.https://www.mdpi.com/2223-7747/11/23/3263genotoxicitymutagenicityoxidative stressyeast<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Bruna B. F. Cal Luana B. N. Araújo Brenno M. Nunes Claudia R. da Silva Marcia B. N. Oliveira Bianka O. Soares Alvaro A. C. Leitão Marcelo de Pádula Debora Nascimento Douglas S. A. Chaves Rachel F. Gagliardi Flavio J. S. Dantas Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast Plants genotoxicity mutagenicity oxidative stress yeast <i>Saccharomyces cerevisiae</i> |
| title | Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast |
| title_full | Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast |
| title_fullStr | Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast |
| title_full_unstemmed | Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast |
| title_short | Cytotoxicity of Extracts from <i>Petiveria alliacea</i> Leaves on Yeast |
| title_sort | cytotoxicity of extracts from i petiveria alliacea i leaves on yeast |
| topic | genotoxicity mutagenicity oxidative stress yeast <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/2223-7747/11/23/3263 |
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