Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus
Ageratum leaf curl Sichuan virus (ALCScV) is a novel monopartite begomovirus, which was identified from Ageratum conyzoides plants in Sichuan Province, China. In this study, we showed that ALCScV can induce typical dwarf and downward leaf-curling symptoms in Ageratum conyzoides, Helianthus annuus, a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.527787/full |
| _version_ | 1819144287124717568 |
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| author | Pengbai Li Chenchen Jing Hongyan Ren Zhou Jia Hussein Ghanem Gentu Wu Mingjun Li Ling Qing |
| author_facet | Pengbai Li Chenchen Jing Hongyan Ren Zhou Jia Hussein Ghanem Gentu Wu Mingjun Li Ling Qing |
| author_sort | Pengbai Li |
| collection | DOAJ |
| description | Ageratum leaf curl Sichuan virus (ALCScV) is a novel monopartite begomovirus, which was identified from Ageratum conyzoides plants in Sichuan Province, China. In this study, we showed that ALCScV can induce typical dwarf and downward leaf-curling symptoms in Ageratum conyzoides, Helianthus annuus, and Nicotiana benthamiana plants and that the noncognate betasatellite can enhance disease symptoms and increase viral accumulation. Expression of the ALCScV-encoded V2, C1, and C4 proteins through a Potato virus X (PVX) vector caused severe symptoms in N. benthamiana. Further study revealed no symptoms in N. benthamiana plants inoculated with infectious ALCScV clones lacking the C4 protein and that the relative viral DNA accumulation levels significantly decreased when compared with ALCScV-inoculated plants. Thus, our mutational analyses demonstrated that C4 is a pathogenicity determinant that plays key roles in symptom formation and virus accumulation. Furthermore, we also demonstrated that the second glycine of C4 was critical for ALCScV pathogenicity. |
| first_indexed | 2024-12-22T12:39:44Z |
| format | Article |
| id | doaj.art-ab288cd357a645d780e3d7f5d62fe2f3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T12:39:44Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ab288cd357a645d780e3d7f5d62fe2f32022-12-21T18:25:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-09-011110.3389/fpls.2020.527787527787Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virusPengbai LiChenchen JingHongyan RenZhou JiaHussein GhanemGentu WuMingjun LiLing QingAgeratum leaf curl Sichuan virus (ALCScV) is a novel monopartite begomovirus, which was identified from Ageratum conyzoides plants in Sichuan Province, China. In this study, we showed that ALCScV can induce typical dwarf and downward leaf-curling symptoms in Ageratum conyzoides, Helianthus annuus, and Nicotiana benthamiana plants and that the noncognate betasatellite can enhance disease symptoms and increase viral accumulation. Expression of the ALCScV-encoded V2, C1, and C4 proteins through a Potato virus X (PVX) vector caused severe symptoms in N. benthamiana. Further study revealed no symptoms in N. benthamiana plants inoculated with infectious ALCScV clones lacking the C4 protein and that the relative viral DNA accumulation levels significantly decreased when compared with ALCScV-inoculated plants. Thus, our mutational analyses demonstrated that C4 is a pathogenicity determinant that plays key roles in symptom formation and virus accumulation. Furthermore, we also demonstrated that the second glycine of C4 was critical for ALCScV pathogenicity.https://www.frontiersin.org/article/10.3389/fpls.2020.527787/fullAgeratum leaf curl Sichuan viruspathogenicitynoncognate betasatelliteC4 proteinN-myristoylationsymptom determinant |
| spellingShingle | Pengbai Li Chenchen Jing Hongyan Ren Zhou Jia Hussein Ghanem Gentu Wu Mingjun Li Ling Qing Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus Frontiers in Plant Science Ageratum leaf curl Sichuan virus pathogenicity noncognate betasatellite C4 protein N-myristoylation symptom determinant |
| title | Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus |
| title_full | Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus |
| title_fullStr | Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus |
| title_full_unstemmed | Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus |
| title_short | Analysis of Pathogenicity and Virulence Factors of Ageratum leaf curl Sichuan virus |
| title_sort | analysis of pathogenicity and virulence factors of ageratum leaf curl sichuan virus |
| topic | Ageratum leaf curl Sichuan virus pathogenicity noncognate betasatellite C4 protein N-myristoylation symptom determinant |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.527787/full |
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