Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration

Sustainable processes accompanied by high extraction yields and minimized amounts of by-products are a major goal of current fruit juice production. Controlled degradation of cell wall polysaccharides, in particular pectin, may contribute to reduced emergence of side streams. Possible strategies for...

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Main Authors: Nicole Jasmin Nemetz, Anne Ruth Winter, Jan-Peter Hensen, Andreas Schieber, Fabian Weber
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Current Research in Food Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2665927123000862
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author Nicole Jasmin Nemetz
Anne Ruth Winter
Jan-Peter Hensen
Andreas Schieber
Fabian Weber
author_facet Nicole Jasmin Nemetz
Anne Ruth Winter
Jan-Peter Hensen
Andreas Schieber
Fabian Weber
author_sort Nicole Jasmin Nemetz
collection DOAJ
description Sustainable processes accompanied by high extraction yields and minimized amounts of by-products are a major goal of current fruit juice production. Controlled degradation of cell wall polysaccharides, in particular pectin, may contribute to reduced emergence of side streams. Possible strategies for the optimization are the selection of enzyme preparations based on comprehensive studies of their activities, the adjustment of maceration temperature toward more gentle conditions, and the application of alternative technologies such as ultrasound (US) during maceration. The present study provides insights into the effects of ultrasound-assisted enzymatic maceration (UAEM) on pectin degradation, total anthocyanin content, thermal and storage stability, and juice yield during chokeberry juice production on pilot-plant scale. The two enzyme preparations applied predominantly possessed polygalacturonase or pectin lyase activity. Cell wall polysaccharide degradation was improved by US and resulted in a 3% increase in juice yield by UAEM using an enzyme preparation that shows mostly polygalacturonase activity. Thermostability of anthocyanins was improved in juices produced using pectin lyase and applying US and matched the stability of anthocyanins in juices produced using polygalacturonase. Storage stability of anthocyanins was improved in juice produced using polygalacturonase during UAEM. UAEM also resulted in lower yields of pomace making the production more resource-efficient. Overall, the use of polygalacturonase has promising potential to advance conventional chokeberry juice production by applying US at gentle conditions.
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spelling doaj.art-ab2a8dd114fd424d8b0a0868fe38d6bd2023-06-22T05:05:11ZengElsevierCurrent Research in Food Science2665-92712023-01-016100518Toward gentle chokeberry juice production by ultrasound-assisted enzymatic macerationNicole Jasmin Nemetz0Anne Ruth Winter1Jan-Peter Hensen2Andreas Schieber3Fabian Weber4Institute of Nutritional and Food Sciences, Molecular Food Technology, University of Bonn, Friedrich-Hirzebruch-Allee 7, D-53115, Bonn, GermanyInstitute of Nutritional and Food Sciences, Molecular Food Technology, University of Bonn, Friedrich-Hirzebruch-Allee 7, D-53115, Bonn, GermanyInstitute of Nutritional and Food Sciences, Molecular Food Technology, University of Bonn, Friedrich-Hirzebruch-Allee 7, D-53115, Bonn, GermanyInstitute of Nutritional and Food Sciences, Molecular Food Technology, University of Bonn, Friedrich-Hirzebruch-Allee 7, D-53115, Bonn, GermanyCorresponding author.; Institute of Nutritional and Food Sciences, Molecular Food Technology, University of Bonn, Friedrich-Hirzebruch-Allee 7, D-53115, Bonn, GermanySustainable processes accompanied by high extraction yields and minimized amounts of by-products are a major goal of current fruit juice production. Controlled degradation of cell wall polysaccharides, in particular pectin, may contribute to reduced emergence of side streams. Possible strategies for the optimization are the selection of enzyme preparations based on comprehensive studies of their activities, the adjustment of maceration temperature toward more gentle conditions, and the application of alternative technologies such as ultrasound (US) during maceration. The present study provides insights into the effects of ultrasound-assisted enzymatic maceration (UAEM) on pectin degradation, total anthocyanin content, thermal and storage stability, and juice yield during chokeberry juice production on pilot-plant scale. The two enzyme preparations applied predominantly possessed polygalacturonase or pectin lyase activity. Cell wall polysaccharide degradation was improved by US and resulted in a 3% increase in juice yield by UAEM using an enzyme preparation that shows mostly polygalacturonase activity. Thermostability of anthocyanins was improved in juices produced using pectin lyase and applying US and matched the stability of anthocyanins in juices produced using polygalacturonase. Storage stability of anthocyanins was improved in juice produced using polygalacturonase during UAEM. UAEM also resulted in lower yields of pomace making the production more resource-efficient. Overall, the use of polygalacturonase has promising potential to advance conventional chokeberry juice production by applying US at gentle conditions.http://www.sciencedirect.com/science/article/pii/S2665927123000862Ultrasound-assisted macerationChokeberry juicePectin degradationPolygalacturonasePectin lyaseAnthocyanin extraction
spellingShingle Nicole Jasmin Nemetz
Anne Ruth Winter
Jan-Peter Hensen
Andreas Schieber
Fabian Weber
Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
Current Research in Food Science
Ultrasound-assisted maceration
Chokeberry juice
Pectin degradation
Polygalacturonase
Pectin lyase
Anthocyanin extraction
title Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
title_full Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
title_fullStr Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
title_full_unstemmed Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
title_short Toward gentle chokeberry juice production by ultrasound-assisted enzymatic maceration
title_sort toward gentle chokeberry juice production by ultrasound assisted enzymatic maceration
topic Ultrasound-assisted maceration
Chokeberry juice
Pectin degradation
Polygalacturonase
Pectin lyase
Anthocyanin extraction
url http://www.sciencedirect.com/science/article/pii/S2665927123000862
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AT anneruthwinter towardgentlechokeberryjuiceproductionbyultrasoundassistedenzymaticmaceration
AT janpeterhensen towardgentlechokeberryjuiceproductionbyultrasoundassistedenzymaticmaceration
AT andreasschieber towardgentlechokeberryjuiceproductionbyultrasoundassistedenzymaticmaceration
AT fabianweber towardgentlechokeberryjuiceproductionbyultrasoundassistedenzymaticmaceration