| Summary: | Currently, several biological activities are attributed to the acetogenins (ACGs) of <i>Annonaceae</i> (including <i>Annona muricata</i>); among these is antimicrobial activity. The main focus of this experiment was to evaluate the antimicrobial effect of the crude extract (CE) and purified acetogenins (P-ACGs) from the endosperm of <i>A. muricata</i> seeds using agar disk diffusion, lethality, sub-lethal, and potential damage membrane tests against Gram-positive and Gram-negative microorganisms. According to the results, P-ACGs present the highest antibacterial effect (12.5–4000 µg/mL) against <i>Enterococcus faecalis</i> (11–15.67 mm), <i>Listeria monocytogenes</i> (12–18 mm), <i>Aeromonas hydrophila</i> (10.33–11.67 mm), <i>Bulkholderia cenocepacia</i> (11–12 mm), and <i>Salmonella paratiphy</i> (11–15.67 mm), and a minimum inhibitory concentration ranging from 0.009 to 12.50 µg/mL. Measurement of the membrane potential shows that, in the presence of P-ACGs, the number of viable cells is reduced, with a significant logarithmic reduction observed (0.38, 1.27, and 1.81 CFU/mL) and a significant sub-lethal lesion (57.78, 96.14, and 98.42%) in <i>Escherichia coli</i>, <i>E. faecalis,</i> and <i>L. monocytogenes</i>, respectively. According to this study, the results demonstrate that P-ACGs from <i>A. muricata</i> seeds are potent and effective antibacterial compounds with potential pharmaceutical applications.
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