Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds
Currently, several biological activities are attributed to the acetogenins (ACGs) of <i>Annonaceae</i> (including <i>Annona muricata</i>); among these is antimicrobial activity. The main focus of this experiment was to evaluate the antimicrobial effect of the crude extract (C...
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MDPI AG
2022-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/1/558 |
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| author | Gabriela Aguilar-Hernández Brandon A. López-Romero Alejandro Pérez-Larios José M. Ruvalcaba-Gómez Inkar Castellanos-Huerta Guillermo Tellez-Isaias Victor M. Petrone-García Luis M. Anaya-Esparza Efigenia Montalvo-González |
| author_facet | Gabriela Aguilar-Hernández Brandon A. López-Romero Alejandro Pérez-Larios José M. Ruvalcaba-Gómez Inkar Castellanos-Huerta Guillermo Tellez-Isaias Victor M. Petrone-García Luis M. Anaya-Esparza Efigenia Montalvo-González |
| author_sort | Gabriela Aguilar-Hernández |
| collection | DOAJ |
| description | Currently, several biological activities are attributed to the acetogenins (ACGs) of <i>Annonaceae</i> (including <i>Annona muricata</i>); among these is antimicrobial activity. The main focus of this experiment was to evaluate the antimicrobial effect of the crude extract (CE) and purified acetogenins (P-ACGs) from the endosperm of <i>A. muricata</i> seeds using agar disk diffusion, lethality, sub-lethal, and potential damage membrane tests against Gram-positive and Gram-negative microorganisms. According to the results, P-ACGs present the highest antibacterial effect (12.5–4000 µg/mL) against <i>Enterococcus faecalis</i> (11–15.67 mm), <i>Listeria monocytogenes</i> (12–18 mm), <i>Aeromonas hydrophila</i> (10.33–11.67 mm), <i>Bulkholderia cenocepacia</i> (11–12 mm), and <i>Salmonella paratiphy</i> (11–15.67 mm), and a minimum inhibitory concentration ranging from 0.009 to 12.50 µg/mL. Measurement of the membrane potential shows that, in the presence of P-ACGs, the number of viable cells is reduced, with a significant logarithmic reduction observed (0.38, 1.27, and 1.81 CFU/mL) and a significant sub-lethal lesion (57.78, 96.14, and 98.42%) in <i>Escherichia coli</i>, <i>E. faecalis,</i> and <i>L. monocytogenes</i>, respectively. According to this study, the results demonstrate that P-ACGs from <i>A. muricata</i> seeds are potent and effective antibacterial compounds with potential pharmaceutical applications. |
| first_indexed | 2024-03-11T10:07:05Z |
| format | Article |
| id | doaj.art-ab2bb0c14ca746fdb05e05a8052476e6 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T10:07:05Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-ab2bb0c14ca746fdb05e05a8052476e62023-11-16T14:58:40ZengMDPI AGApplied Sciences2076-34172022-12-0113155810.3390/app13010558Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> SeedsGabriela Aguilar-Hernández0Brandon A. López-Romero1Alejandro Pérez-Larios2José M. Ruvalcaba-Gómez3Inkar Castellanos-Huerta4Guillermo Tellez-Isaias5Victor M. Petrone-García6Luis M. Anaya-Esparza7Efigenia Montalvo-González8División de Ciencias Agropecuarias e Ingenierías, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de Morelos 47600, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Tepic 63175, MexicoDivisión de Ciencias Agropecuarias e Ingenierías, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de Morelos 47600, MexicoCentro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Tepatitlán de Morelos 47600, MexicoPrograma de Maestria y Doctorado en Ciencias de la Producción y de la Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico 04510, MexicoDepartment of Poultry Science, University of Arkansas, Fayetteville, AK 72701, USADepartamento de Ciencias Pecuarias, Facultad de Estudios Superiores Cuautitlán UNAM, Cuautitlán 54714, MexicoDivisión de Ciencias Agropecuarias e Ingenierías, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitlán de Morelos 47600, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Tepic 63175, MexicoCurrently, several biological activities are attributed to the acetogenins (ACGs) of <i>Annonaceae</i> (including <i>Annona muricata</i>); among these is antimicrobial activity. The main focus of this experiment was to evaluate the antimicrobial effect of the crude extract (CE) and purified acetogenins (P-ACGs) from the endosperm of <i>A. muricata</i> seeds using agar disk diffusion, lethality, sub-lethal, and potential damage membrane tests against Gram-positive and Gram-negative microorganisms. According to the results, P-ACGs present the highest antibacterial effect (12.5–4000 µg/mL) against <i>Enterococcus faecalis</i> (11–15.67 mm), <i>Listeria monocytogenes</i> (12–18 mm), <i>Aeromonas hydrophila</i> (10.33–11.67 mm), <i>Bulkholderia cenocepacia</i> (11–12 mm), and <i>Salmonella paratiphy</i> (11–15.67 mm), and a minimum inhibitory concentration ranging from 0.009 to 12.50 µg/mL. Measurement of the membrane potential shows that, in the presence of P-ACGs, the number of viable cells is reduced, with a significant logarithmic reduction observed (0.38, 1.27, and 1.81 CFU/mL) and a significant sub-lethal lesion (57.78, 96.14, and 98.42%) in <i>Escherichia coli</i>, <i>E. faecalis,</i> and <i>L. monocytogenes</i>, respectively. According to this study, the results demonstrate that P-ACGs from <i>A. muricata</i> seeds are potent and effective antibacterial compounds with potential pharmaceutical applications.https://www.mdpi.com/2076-3417/13/1/558<i>A. muricata</i>seedsacetogeninsnatural extractsantimicrobial activity |
| spellingShingle | Gabriela Aguilar-Hernández Brandon A. López-Romero Alejandro Pérez-Larios José M. Ruvalcaba-Gómez Inkar Castellanos-Huerta Guillermo Tellez-Isaias Victor M. Petrone-García Luis M. Anaya-Esparza Efigenia Montalvo-González Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds Applied Sciences <i>A. muricata</i> seeds acetogenins natural extracts antimicrobial activity |
| title | Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds |
| title_full | Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds |
| title_fullStr | Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds |
| title_full_unstemmed | Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds |
| title_short | Antibacterial Activity of Crude Extract and Purified Acetogenins from <i>Annona muricata</i> Seeds |
| title_sort | antibacterial activity of crude extract and purified acetogenins from i annona muricata i seeds |
| topic | <i>A. muricata</i> seeds acetogenins natural extracts antimicrobial activity |
| url | https://www.mdpi.com/2076-3417/13/1/558 |
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