Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center
Background and Objectives: Stored blood undergoes many metabolic, biochemical, and molecular changes known as storage lesions which are responsible for negative posttransfusion effects in recipients. This study aims to detect serial in vitro qualitative changes that occur during processing and blood...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Global Journal of Transfusion Medicine |
| Subjects: | |
| Online Access: | http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2023;volume=8;issue=2;spage=190;epage=196;aulast=Gaur |
| _version_ | 1797352179895369728 |
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| author | Parisha Gaur Aparna Bhardwaj Gaurav Raturi Saqib Ahmed Rana Usmani |
| author_facet | Parisha Gaur Aparna Bhardwaj Gaurav Raturi Saqib Ahmed Rana Usmani |
| author_sort | Parisha Gaur |
| collection | DOAJ |
| description | Background and Objectives: Stored blood undergoes many metabolic, biochemical, and molecular changes known as storage lesions which are responsible for negative posttransfusion effects in recipients. This study aims to detect serial in vitro qualitative changes that occur during processing and blood storage including changes in plasma hemoglobin (Hb), plasma lactate dehydrogenase (LDH), and potassium levels and changes (fall) in pH of packed red blood cell at serial intervals under a standard condition of storage in the blood bank. This is a two year prospective study. Methods: Fifty donors were selected and blood units were collected and stored under blood bank conditions. Mean plasma Hb of stored blood was estimated by measuring the transmittance and absorbance of modified azide-methemoglobin and percentage hemolysis was calculated on days 0, 1, 7, 21, 28, 35, and 42 days. Similarly, plasma LDH and plasma potassium levels were also assessed during storage. Results: It was noted that free Hb level and percentage hemolysis progressively increased with storage along with the level of LDH and potassium. However, the extent of hemolysis did not exceed the permissible limit of up to 42 days of storage. Conclusion: It was concluded that quantitative estimation of Hb is superior to the visual method for the determination of hemolysis. Hemolysis can be further reduced by additive solution saline, adenine, glucose, and mannitol and using di-2-ethyl hexyl pHthalate as s plasticizer in blood bags for storage. |
| first_indexed | 2024-03-08T13:11:33Z |
| format | Article |
| id | doaj.art-ab2d5fc172ae4b06bb866154cb6263ed |
| institution | Directory Open Access Journal |
| issn | 2468-8398 2455-8893 |
| language | English |
| last_indexed | 2024-03-08T13:11:33Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Global Journal of Transfusion Medicine |
| spelling | doaj.art-ab2d5fc172ae4b06bb866154cb6263ed2024-01-18T12:17:55ZengWolters Kluwer Medknow PublicationsGlobal Journal of Transfusion Medicine2468-83982455-88932023-01-018219019610.4103/gjtm.gjtm_10_23Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care centerParisha GaurAparna BhardwajGaurav RaturiSaqib AhmedRana UsmaniBackground and Objectives: Stored blood undergoes many metabolic, biochemical, and molecular changes known as storage lesions which are responsible for negative posttransfusion effects in recipients. This study aims to detect serial in vitro qualitative changes that occur during processing and blood storage including changes in plasma hemoglobin (Hb), plasma lactate dehydrogenase (LDH), and potassium levels and changes (fall) in pH of packed red blood cell at serial intervals under a standard condition of storage in the blood bank. This is a two year prospective study. Methods: Fifty donors were selected and blood units were collected and stored under blood bank conditions. Mean plasma Hb of stored blood was estimated by measuring the transmittance and absorbance of modified azide-methemoglobin and percentage hemolysis was calculated on days 0, 1, 7, 21, 28, 35, and 42 days. Similarly, plasma LDH and plasma potassium levels were also assessed during storage. Results: It was noted that free Hb level and percentage hemolysis progressively increased with storage along with the level of LDH and potassium. However, the extent of hemolysis did not exceed the permissible limit of up to 42 days of storage. Conclusion: It was concluded that quantitative estimation of Hb is superior to the visual method for the determination of hemolysis. Hemolysis can be further reduced by additive solution saline, adenine, glucose, and mannitol and using di-2-ethyl hexyl pHthalate as s plasticizer in blood bags for storage.http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2023;volume=8;issue=2;spage=190;epage=196;aulast=Gaurbiochemical markersblood bankblood storagehemolysis |
| spellingShingle | Parisha Gaur Aparna Bhardwaj Gaurav Raturi Saqib Ahmed Rana Usmani Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center Global Journal of Transfusion Medicine biochemical markers blood bank blood storage hemolysis |
| title | Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center |
| title_full | Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center |
| title_fullStr | Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center |
| title_full_unstemmed | Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center |
| title_short | Correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health-care center |
| title_sort | correlation of hemolysis by plasma hemoglobin with biochemical markers during storage of blood under standard conditions in the blood bank of a tertiary health care center |
| topic | biochemical markers blood bank blood storage hemolysis |
| url | http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2023;volume=8;issue=2;spage=190;epage=196;aulast=Gaur |
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