A framework for real-time monitoring, analysis and adaptive sampling of viral amplicon nanopore sequencing

The ongoing SARS-CoV-2 pandemic demonstrates the utility of real-time sequence analysis in monitoring and surveillance of pathogens. However, cost-effective sequencing requires that samples be PCR amplified and multiplexed via barcoding onto a single flow cell, resulting in challenges with maximisin...

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Hlavní autoři: Rory Munro, Nadine Holmes, Christopher Moore, Matthew Carlile, Alexander Payne, John R. Tyson, Thomas Williams, Christopher Alder, Luke B. Snell, Gaia Nebbia, Roberto Santos, Matt Loose
Médium: Článek
Jazyk:English
Vydáno: Frontiers Media S.A. 2023-03-01
Edice:Frontiers in Genetics
Témata:
On-line přístup:https://www.frontiersin.org/articles/10.3389/fgene.2023.1138582/full