Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate
Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N rati...
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| Format: | Article |
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Elsevier
2014-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913603482 |
| _version_ | 1818839084938821632 |
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| author | Xiang ZHANG Chun-hua LÜ Yuan CHEN Gui-xia WANG Yuan CHEN De-hua CHEN |
| author_facet | Xiang ZHANG Chun-hua LÜ Yuan CHEN Gui-xia WANG Yuan CHEN De-hua CHEN |
| author_sort | Xiang ZHANG |
| collection | DOAJ |
| description | Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha−1, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of insectiocidal efficacy in Bt cotton, especially in peak boll period. |
| first_indexed | 2024-12-19T03:48:40Z |
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| id | doaj.art-ab3257f4d46242d68551141a3efa571a |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T03:48:40Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-ab3257f4d46242d68551141a3efa571a2022-12-21T20:37:04ZengElsevierJournal of Integrative Agriculture2095-31192014-01-011318288Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N RateXiang ZHANG0Chun-hua LÜ1Yuan CHEN2Gui-xia WANG3Yuan CHEN4De-hua CHEN5Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.ChinaJiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.ChinaDepartment of Soil and Crop Sciences, Texas A&M University, College Station 77843, USAJiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.ChinaJiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.ChinaJiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence CHEN De-hua, Tel: +86-514-87979357, Fax: +86-514-87996817Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha−1, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of insectiocidal efficacy in Bt cotton, especially in peak boll period.http://www.sciencedirect.com/science/article/pii/S2095311913603482Bt cottonC/N ratioBt proteininsecticidal efficacy |
| spellingShingle | Xiang ZHANG Chun-hua LÜ Yuan CHEN Gui-xia WANG Yuan CHEN De-hua CHEN Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate Journal of Integrative Agriculture Bt cotton C/N ratio Bt protein insecticidal efficacy |
| title | Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| title_full | Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| title_fullStr | Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| title_full_unstemmed | Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| title_short | Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| title_sort | relationship between leaf c n ratio and insecticidal protein expression in bt cotton as affected by high temperature and n rate |
| topic | Bt cotton C/N ratio Bt protein insecticidal efficacy |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913603482 |
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