Sensitive red protein calcium indicators for imaging neural activity
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/12727 |
| _version_ | 1811180431309012992 |
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| author | Hod Dana Boaz Mohar Yi Sun Sujatha Narayan Andrew Gordus Jeremy P Hasseman Getahun Tsegaye Graham T Holt Amy Hu Deepika Walpita Ronak Patel John J Macklin Cornelia I Bargmann Misha B Ahrens Eric R Schreiter Vivek Jayaraman Loren L Looger Karel Svoboda Douglas S Kim |
| author_facet | Hod Dana Boaz Mohar Yi Sun Sujatha Narayan Andrew Gordus Jeremy P Hasseman Getahun Tsegaye Graham T Holt Amy Hu Deepika Walpita Ronak Patel John J Macklin Cornelia I Bargmann Misha B Ahrens Eric R Schreiter Vivek Jayaraman Loren L Looger Karel Svoboda Douglas S Kim |
| author_sort | Hod Dana |
| collection | DOAJ |
| description | Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging. |
| first_indexed | 2024-04-11T09:03:02Z |
| format | Article |
| id | doaj.art-ab34ae2c5e2b47b2a8b288f3d69d627e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:03:02Z |
| publishDate | 2016-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ab34ae2c5e2b47b2a8b288f3d69d627e2022-12-22T04:32:43ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.12727Sensitive red protein calcium indicators for imaging neural activityHod Dana0Boaz Mohar1Yi Sun2Sujatha Narayan3Andrew Gordus4Jeremy P Hasseman5Getahun Tsegaye6Graham T Holt7Amy Hu8Deepika Walpita9Ronak Patel10John J Macklin11Cornelia I Bargmann12https://orcid.org/0000-0002-8484-0618Misha B Ahrens13https://orcid.org/0000-0002-3457-4462Eric R Schreiter14Vivek Jayaraman15Loren L Looger16Karel Svoboda17https://orcid.org/0000-0002-6670-7362Douglas S Kim18https://orcid.org/0000-0001-9029-314XJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States; Weizmann Institute of Science, Rehovot, IsraelJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesHoward Hughes Medical Institute, The Rockefeller University, New York, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesHoward Hughes Medical Institute, The Rockefeller University, New York, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesGenetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.https://elifesciences.org/articles/12727GECIfluorescent probescalcium imagingprotein engineering |
| spellingShingle | Hod Dana Boaz Mohar Yi Sun Sujatha Narayan Andrew Gordus Jeremy P Hasseman Getahun Tsegaye Graham T Holt Amy Hu Deepika Walpita Ronak Patel John J Macklin Cornelia I Bargmann Misha B Ahrens Eric R Schreiter Vivek Jayaraman Loren L Looger Karel Svoboda Douglas S Kim Sensitive red protein calcium indicators for imaging neural activity eLife GECI fluorescent probes calcium imaging protein engineering |
| title | Sensitive red protein calcium indicators for imaging neural activity |
| title_full | Sensitive red protein calcium indicators for imaging neural activity |
| title_fullStr | Sensitive red protein calcium indicators for imaging neural activity |
| title_full_unstemmed | Sensitive red protein calcium indicators for imaging neural activity |
| title_short | Sensitive red protein calcium indicators for imaging neural activity |
| title_sort | sensitive red protein calcium indicators for imaging neural activity |
| topic | GECI fluorescent probes calcium imaging protein engineering |
| url | https://elifesciences.org/articles/12727 |
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