Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i>
The putative methyltransferase Lae1 is a global regulator in <i>Trichoderma,</i> which modulates the expression of secondary metabolite gene clusters, possibly via chromatin remodeling. Here we aimed to explore the specific transcription and metabolites profiles regulated by Lae1 in <...
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MDPI AG
2023-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/120 |
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| author | Yanxiang Shen Yiwen Zhang Hui Zhang Xinhua Wang Jie Chen Yaqian Li |
| author_facet | Yanxiang Shen Yiwen Zhang Hui Zhang Xinhua Wang Jie Chen Yaqian Li |
| author_sort | Yanxiang Shen |
| collection | DOAJ |
| description | The putative methyltransferase Lae1 is a global regulator in <i>Trichoderma,</i> which modulates the expression of secondary metabolite gene clusters, possibly via chromatin remodeling. Here we aimed to explore the specific transcription and metabolites profiles regulated by Lae1 in <i>T. atroviride</i> 23. Comparative transcriptomics and metabolome analyses between the <i>lae1</i> deletion (Mlae1) and over-expressing (Olae1) mutants were performed using RNA sequencing and QTOF-UPLC-MS techniques. In total, 1344 unique differentially expressed genes (DEGs) and 92 metabolites were identified across three strains. The significantly altered metabolic profiles revealed that the <i>lae1</i> gene modulates central carbon metabolism, amino acid metabolism, secondary metabolism, and phospholipid metabolism. The effects of <i>lae1</i> on phospholipid metabolism were further explored, and the findings showed that <i>lae1</i> modulates the composition and function of cell membranes and other metabolic activities, including the phosphotransferase system (PTS) and biosynthesis of secondary metabolites (SM). Phospholipid metabolism is related to energy metabolism, signal transduction, and environmental adaptability of microorganisms. These data showed that <i>Lae1</i> affects the primary metabolites, phospholipid, as well as the regulation of secondary metabolites in Trichoderma. This study could potentially provoke in-depth investigations of the Lae1-mediated target genes in phospholipid synthesis. The Lae1 may act as a novel target that is associated with disease defense and drug development in the future. |
| first_indexed | 2024-03-09T12:03:58Z |
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| id | doaj.art-ab352c9d4b8e46008a6d5a7dbb667e25 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:03:58Z |
| publishDate | 2023-01-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-ab352c9d4b8e46008a6d5a7dbb667e252023-11-30T23:00:36ZengMDPI AGJournal of Fungi2309-608X2023-01-019112010.3390/jof9010120Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i>Yanxiang Shen0Yiwen Zhang1Hui Zhang2Xinhua Wang3Jie Chen4Yaqian Li5School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaThe putative methyltransferase Lae1 is a global regulator in <i>Trichoderma,</i> which modulates the expression of secondary metabolite gene clusters, possibly via chromatin remodeling. Here we aimed to explore the specific transcription and metabolites profiles regulated by Lae1 in <i>T. atroviride</i> 23. Comparative transcriptomics and metabolome analyses between the <i>lae1</i> deletion (Mlae1) and over-expressing (Olae1) mutants were performed using RNA sequencing and QTOF-UPLC-MS techniques. In total, 1344 unique differentially expressed genes (DEGs) and 92 metabolites were identified across three strains. The significantly altered metabolic profiles revealed that the <i>lae1</i> gene modulates central carbon metabolism, amino acid metabolism, secondary metabolism, and phospholipid metabolism. The effects of <i>lae1</i> on phospholipid metabolism were further explored, and the findings showed that <i>lae1</i> modulates the composition and function of cell membranes and other metabolic activities, including the phosphotransferase system (PTS) and biosynthesis of secondary metabolites (SM). Phospholipid metabolism is related to energy metabolism, signal transduction, and environmental adaptability of microorganisms. These data showed that <i>Lae1</i> affects the primary metabolites, phospholipid, as well as the regulation of secondary metabolites in Trichoderma. This study could potentially provoke in-depth investigations of the Lae1-mediated target genes in phospholipid synthesis. The Lae1 may act as a novel target that is associated with disease defense and drug development in the future.https://www.mdpi.com/2309-608X/9/1/120<i>Trichoderma atroviride</i>methyltransferase <i>lae1</i>RNA-sequence transcriptomeLC-MS/MS metabolomephospholipid metabolism |
| spellingShingle | Yanxiang Shen Yiwen Zhang Hui Zhang Xinhua Wang Jie Chen Yaqian Li Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> Journal of Fungi <i>Trichoderma atroviride</i> methyltransferase <i>lae1</i> RNA-sequence transcriptome LC-MS/MS metabolome phospholipid metabolism |
| title | Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> |
| title_full | Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> |
| title_fullStr | Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> |
| title_full_unstemmed | Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> |
| title_short | Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism in <i>Trichoderma atroviride</i> |
| title_sort | integrated transcriptome and untargeted metabolomic analyses revealed the role of methyltransferase lae1 in the regulation of phospholipid metabolism in i trichoderma atroviride i |
| topic | <i>Trichoderma atroviride</i> methyltransferase <i>lae1</i> RNA-sequence transcriptome LC-MS/MS metabolome phospholipid metabolism |
| url | https://www.mdpi.com/2309-608X/9/1/120 |
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