Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation
Abstract Lysophosphatidylcholine (LPC) was previously found to show neuroprotective effect on nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) induced signalings. Also, numerous studies reported the emerging roles of long noncoding RNAs (LncRNAs) involved in neurodegenerative d...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21676-5 |
| _version_ | 1811230488489099264 |
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| author | Wen Zhang Su Dun Yin Ping Qingliang Wang Siqin Tana Aodong Tana Si Qin Xilinqiqige Bao Alateng Qimuge Tegexi Baiyin Dezhi Yang Siqin Bao Seyin Baoyin Wuhan Qimuge |
| author_facet | Wen Zhang Su Dun Yin Ping Qingliang Wang Siqin Tana Aodong Tana Si Qin Xilinqiqige Bao Alateng Qimuge Tegexi Baiyin Dezhi Yang Siqin Bao Seyin Baoyin Wuhan Qimuge |
| author_sort | Wen Zhang |
| collection | DOAJ |
| description | Abstract Lysophosphatidylcholine (LPC) was previously found to show neuroprotective effect on nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) induced signalings. Also, numerous studies reported the emerging roles of long noncoding RNAs (LncRNAs) involved in neurodegenerative disease. However, the biological mechanism of LPC and expression profile of lncRNAs has not been reported. Here, lncRNAs in PC12 cells under LPC and NGF treatment were analyzed using high throughput sequencing technology for the first time. We identified 564 annotated and 1077 novel lncRNAs in PC12 cells. Among them, 121 lncRNAs were differentially expressed in the PC12 cells under LPC stimulation. KEGG analysis showed that differentially expressed mRNAs co-expressed with lncRNAs mainly enriched in ribosome, oxidative phosphorylation, Parkinson’s disease, Huntington’s disease and Alzheimer’s disease etc. LncRNA-mRNA network analysis showed that lncRNA ENSRNOT00000082515 had interactions with 626 different mRNAs suggesting that lncRNA ENSRNOT00000082515 probably play vital role. Finally, sequencing data were validated by qRT-PCR for ENSRNOT00000084874, ENSRNOT00000082515, LNC_001033 forward Fgf18, Vcam1, and Pck2. |
| first_indexed | 2024-04-12T10:30:48Z |
| format | Article |
| id | doaj.art-ab358f99ba4143729e7e9e11e522f172 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T10:30:48Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ab358f99ba4143729e7e9e11e522f1722022-12-22T03:36:52ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-21676-5Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulationWen Zhang0Su Dun1Yin Ping2Qingliang Wang3Siqin Tana4Aodong Tana5Si Qin6Xilinqiqige Bao7Alateng Qimuge8Tegexi Baiyin9Dezhi Yang10Siqin Bao11Seyin Baoyin12Wuhan Qimuge13School of Life Science, Inner Mongolia UniversityResearch and Development Center, HUA Cloud Intelligent Healthcare Co., LtdAffiliated Hospital of Inner Mongolia University for NationalitiesInner Mongolia Medical UniversityInner Mongolia International Mongolian HospitalInner Mongolia International Mongolian HospitalInner Mongolia International Mongolian HospitalInner Mongolia Medical UniversityInner Mongolia International Mongolian HospitalAffiliated Hospital of Inner Mongolia University for NationalitiesInner Mongolia International Mongolian HospitalInner Mongolia International Mongolian HospitalInner Mongolia International Mongolian HospitalInner Mongolia International Mongolian HospitalAbstract Lysophosphatidylcholine (LPC) was previously found to show neuroprotective effect on nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) induced signalings. Also, numerous studies reported the emerging roles of long noncoding RNAs (LncRNAs) involved in neurodegenerative disease. However, the biological mechanism of LPC and expression profile of lncRNAs has not been reported. Here, lncRNAs in PC12 cells under LPC and NGF treatment were analyzed using high throughput sequencing technology for the first time. We identified 564 annotated and 1077 novel lncRNAs in PC12 cells. Among them, 121 lncRNAs were differentially expressed in the PC12 cells under LPC stimulation. KEGG analysis showed that differentially expressed mRNAs co-expressed with lncRNAs mainly enriched in ribosome, oxidative phosphorylation, Parkinson’s disease, Huntington’s disease and Alzheimer’s disease etc. LncRNA-mRNA network analysis showed that lncRNA ENSRNOT00000082515 had interactions with 626 different mRNAs suggesting that lncRNA ENSRNOT00000082515 probably play vital role. Finally, sequencing data were validated by qRT-PCR for ENSRNOT00000084874, ENSRNOT00000082515, LNC_001033 forward Fgf18, Vcam1, and Pck2.https://doi.org/10.1038/s41598-022-21676-5 |
| spellingShingle | Wen Zhang Su Dun Yin Ping Qingliang Wang Siqin Tana Aodong Tana Si Qin Xilinqiqige Bao Alateng Qimuge Tegexi Baiyin Dezhi Yang Siqin Bao Seyin Baoyin Wuhan Qimuge Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation Scientific Reports |
| title | Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation |
| title_full | Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation |
| title_fullStr | Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation |
| title_full_unstemmed | Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation |
| title_short | Differentially expressed long noncoding RNAs and mRNAs in PC12 cells under lysophosphatidylcholine stimulation |
| title_sort | differentially expressed long noncoding rnas and mrnas in pc12 cells under lysophosphatidylcholine stimulation |
| url | https://doi.org/10.1038/s41598-022-21676-5 |
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