High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia
<i>Klebsiella pneumoniae</i> poses an urgent public health threat, causing nosocomial outbreaks in different continents. It has been observed to develop resistance to antimicrobials more easily than most bacteria. These days, multidrug-resistant strains are being increasingly reported fr...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/8/1007 |
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| author | Tewachew Awoke Brhanu Teka Aminu Seman Shemse Sebre Biruk Yeshitela Abraham Aseffa Adane Mihret Tamrat Abebe |
| author_facet | Tewachew Awoke Brhanu Teka Aminu Seman Shemse Sebre Biruk Yeshitela Abraham Aseffa Adane Mihret Tamrat Abebe |
| author_sort | Tewachew Awoke |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> poses an urgent public health threat, causing nosocomial outbreaks in different continents. It has been observed to develop resistance to antimicrobials more easily than most bacteria. These days, multidrug-resistant strains are being increasingly reported from different countries. However, studies on the surveillance of multidrug-resistant <i>Klebsiella pneumoniae</i> are very rare in Ethiopia. This study aimed to determine the antimicrobial resistance patterns and magnitude of MDR <i>K. pneumoniae</i> isolates from patients attending or admitted to Tikur Anbessa Specialized Hospital (TASH). A cross-sectional study was conducted from September 2018 to February 2019 at TASH, Addis Ababa, Ethiopia. Identification of <i>K. pneumoniae</i> was done by examining the Gram stain, colony characteristics on MacConkey agar and 5% sheep blood agar, as well as using a series of biochemical tests. Antimicrobial susceptibility testing of the isolates for 21 antimicrobials was done by the Kirby–Bauer disc diffusion technique. Data were double entered using Epidata 3.1 and exported to SPSS version 25 software for analysis. Among the total <i>K. pneumoniae</i> isolates (<i>n</i> = 132), almost all 130 (98.5%) were MDR. Two (1.5%) isolates showed complete non-susceptibility to all antimicrobial agents tested. Moreover, a high rate of resistance was observed to cefotaxime and ceftriaxone 128 (97%), trimethoprim-sulfamethoxazole 124 (93.9%), and cefepime 111 (84.1%). High susceptibility was recorded to amikacin 123 (93.2%), imipenem 107 (81.1%), meropenem 96 (72.7%), and ertapenem 93 (70.5%). <i>K. pneumoniae</i> isolates showed a high rate of resistance to most of the tested antimicrobials. The magnitude of MDR <i>K. pneumoniae</i> was very alarming. Therefore, strengthening antimicrobial stewardship programs and antimicrobial surveillance practices is strongly recommended in TASH. |
| first_indexed | 2024-03-10T09:03:34Z |
| format | Article |
| id | doaj.art-ab3678598eb14bc1b39f39c7d23a88be |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:03:34Z |
| publishDate | 2021-08-01 |
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| series | Antibiotics |
| spelling | doaj.art-ab3678598eb14bc1b39f39c7d23a88be2023-11-22T06:34:35ZengMDPI AGAntibiotics2079-63822021-08-01108100710.3390/antibiotics10081007High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in EthiopiaTewachew Awoke0Brhanu Teka1Aminu Seman2Shemse Sebre3Biruk Yeshitela4Abraham Aseffa5Adane Mihret6Tamrat Abebe7Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar 79, EthiopiaDepartment of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa 9086, EthiopiaDepartment of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa 9086, EthiopiaDepartment of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa 9086, EthiopiaBacteriology Unit, Armauer Hansen Research Institute, Addis Ababa 1005, EthiopiaBacteriology Unit, Armauer Hansen Research Institute, Addis Ababa 1005, EthiopiaDepartment of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa 9086, EthiopiaDepartment of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa 9086, Ethiopia<i>Klebsiella pneumoniae</i> poses an urgent public health threat, causing nosocomial outbreaks in different continents. It has been observed to develop resistance to antimicrobials more easily than most bacteria. These days, multidrug-resistant strains are being increasingly reported from different countries. However, studies on the surveillance of multidrug-resistant <i>Klebsiella pneumoniae</i> are very rare in Ethiopia. This study aimed to determine the antimicrobial resistance patterns and magnitude of MDR <i>K. pneumoniae</i> isolates from patients attending or admitted to Tikur Anbessa Specialized Hospital (TASH). A cross-sectional study was conducted from September 2018 to February 2019 at TASH, Addis Ababa, Ethiopia. Identification of <i>K. pneumoniae</i> was done by examining the Gram stain, colony characteristics on MacConkey agar and 5% sheep blood agar, as well as using a series of biochemical tests. Antimicrobial susceptibility testing of the isolates for 21 antimicrobials was done by the Kirby–Bauer disc diffusion technique. Data were double entered using Epidata 3.1 and exported to SPSS version 25 software for analysis. Among the total <i>K. pneumoniae</i> isolates (<i>n</i> = 132), almost all 130 (98.5%) were MDR. Two (1.5%) isolates showed complete non-susceptibility to all antimicrobial agents tested. Moreover, a high rate of resistance was observed to cefotaxime and ceftriaxone 128 (97%), trimethoprim-sulfamethoxazole 124 (93.9%), and cefepime 111 (84.1%). High susceptibility was recorded to amikacin 123 (93.2%), imipenem 107 (81.1%), meropenem 96 (72.7%), and ertapenem 93 (70.5%). <i>K. pneumoniae</i> isolates showed a high rate of resistance to most of the tested antimicrobials. The magnitude of MDR <i>K. pneumoniae</i> was very alarming. Therefore, strengthening antimicrobial stewardship programs and antimicrobial surveillance practices is strongly recommended in TASH.https://www.mdpi.com/2079-6382/10/8/1007<i>K. pneumoniae</i>antimicrobial susceptibility patternsMDREthiopia |
| spellingShingle | Tewachew Awoke Brhanu Teka Aminu Seman Shemse Sebre Biruk Yeshitela Abraham Aseffa Adane Mihret Tamrat Abebe High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia Antibiotics <i>K. pneumoniae</i> antimicrobial susceptibility patterns MDR Ethiopia |
| title | High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia |
| title_full | High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia |
| title_fullStr | High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia |
| title_full_unstemmed | High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia |
| title_short | High Prevalence of Multidrug-Resistant <i>Klebsiella pneumoniae</i> in a Tertiary Care Hospital in Ethiopia |
| title_sort | high prevalence of multidrug resistant i klebsiella pneumoniae i in a tertiary care hospital in ethiopia |
| topic | <i>K. pneumoniae</i> antimicrobial susceptibility patterns MDR Ethiopia |
| url | https://www.mdpi.com/2079-6382/10/8/1007 |
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