Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy
Shuang Zhao,1,* Litao Ma,1,* Chengwen Cao,1 Qianqian Yu,1 Lanmei Chen,1,2 Jie Liu1 1Department of Chemistry, Jinan University, Guangzhou, 2Department of Chemistry, School of Pharmacy, Guangdong Medical University, Zhanjiang, People’s Republic of China *These authors contributed equally t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-03-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/curcumin-loaded-redox-response--of-self-assembled-micelles-for-enhance-peer-reviewed-article-IJN |
| _version_ | 1818347089892999168 |
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| author | Zhao S Ma L Cao C Yu Q Chen L Liu J |
| author_facet | Zhao S Ma L Cao C Yu Q Chen L Liu J |
| author_sort | Zhao S |
| collection | DOAJ |
| description | Shuang Zhao,1,* Litao Ma,1,* Chengwen Cao,1 Qianqian Yu,1 Lanmei Chen,1,2 Jie Liu1 1Department of Chemistry, Jinan University, Guangzhou, 2Department of Chemistry, School of Pharmacy, Guangdong Medical University, Zhanjiang, People’s Republic of China *These authors contributed equally to this work Abstract: At present, it has become evident that inflammation plays a critical role in tumor growth; meanwhile, chemotherapeutic agents using nanocarriers have been suggested as a promising strategy in cancer treatment. In this study, novel redox-responsive micelles were prepared from monomethoxy-poly(ethylene glycol)-chitosan-S-S-hexadecyl (C16-SS-CS-mPEG). These micelles were able to carry and deliver drugs into tumor cells. To serve as a control, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexadecyl (C16-CC-CS-mPEG) was developed in a similar fashion to that used to yield C16-CC-CS-mPEG without a redox-responsive disulfide bond. The cellular uptake mechanisms of both micelles were determined. The efficient intracellular drug release from micelles in MCF-7 cells was further confirmed. Results indicated that curcumin (Cur) could rapidly form C16-SS-CS-mPEG@Cur micelles when exposed to reducing agents and efficaciously enhance intracellular accumulation. The cytotoxicity assay demonstrated that C16-SS-CS-mPEG@Cur exhibited satisfactory cytotoxicity against MCF-7 cells. Anti-inflammation assay results indicated that C16-SS-CS-mPEG@Cur treatment significantly downregulated tumor necrosis factor (TNF-α) expression and showed good anti-inflammatory effects in tumor microenvironment. Most importantly, antitumor effects in vivo showed satisfactory therapeutic effects with C16-SS-CS-mPEG@Cur. Hence, C16-SS-CS-mPEG@Cur micelles can be useful in tumor therapy. Keywords: micelles, curcumin, anti-inflammatory effect, anti-tumor effect, tumor |
| first_indexed | 2024-12-13T17:28:37Z |
| format | Article |
| id | doaj.art-ab37604def8a412384d1d825bb2bc622 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-13T17:28:37Z |
| publishDate | 2017-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-ab37604def8a412384d1d825bb2bc6222022-12-21T23:37:07ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-03-01Volume 122489250432109Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacyZhao SMa LCao CYu QChen LLiu JShuang Zhao,1,* Litao Ma,1,* Chengwen Cao,1 Qianqian Yu,1 Lanmei Chen,1,2 Jie Liu1 1Department of Chemistry, Jinan University, Guangzhou, 2Department of Chemistry, School of Pharmacy, Guangdong Medical University, Zhanjiang, People’s Republic of China *These authors contributed equally to this work Abstract: At present, it has become evident that inflammation plays a critical role in tumor growth; meanwhile, chemotherapeutic agents using nanocarriers have been suggested as a promising strategy in cancer treatment. In this study, novel redox-responsive micelles were prepared from monomethoxy-poly(ethylene glycol)-chitosan-S-S-hexadecyl (C16-SS-CS-mPEG). These micelles were able to carry and deliver drugs into tumor cells. To serve as a control, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexadecyl (C16-CC-CS-mPEG) was developed in a similar fashion to that used to yield C16-CC-CS-mPEG without a redox-responsive disulfide bond. The cellular uptake mechanisms of both micelles were determined. The efficient intracellular drug release from micelles in MCF-7 cells was further confirmed. Results indicated that curcumin (Cur) could rapidly form C16-SS-CS-mPEG@Cur micelles when exposed to reducing agents and efficaciously enhance intracellular accumulation. The cytotoxicity assay demonstrated that C16-SS-CS-mPEG@Cur exhibited satisfactory cytotoxicity against MCF-7 cells. Anti-inflammation assay results indicated that C16-SS-CS-mPEG@Cur treatment significantly downregulated tumor necrosis factor (TNF-α) expression and showed good anti-inflammatory effects in tumor microenvironment. Most importantly, antitumor effects in vivo showed satisfactory therapeutic effects with C16-SS-CS-mPEG@Cur. Hence, C16-SS-CS-mPEG@Cur micelles can be useful in tumor therapy. Keywords: micelles, curcumin, anti-inflammatory effect, anti-tumor effect, tumorhttps://www.dovepress.com/curcumin-loaded-redox-response--of-self-assembled-micelles-for-enhance-peer-reviewed-article-IJNKeywords: Micelles Curcumin Anti-inflammatory effecta Anti-tumor effecta Tumor |
| spellingShingle | Zhao S Ma L Cao C Yu Q Chen L Liu J Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy International Journal of Nanomedicine Keywords: Micelles Curcumin Anti-inflammatory effecta Anti-tumor effecta Tumor |
| title | Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy |
| title_full | Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy |
| title_fullStr | Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy |
| title_full_unstemmed | Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy |
| title_short | Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy |
| title_sort | curcumin loaded redox response of self assembled micelles for enhanced antitumor and anti inflammation efficacy |
| topic | Keywords: Micelles Curcumin Anti-inflammatory effecta Anti-tumor effecta Tumor |
| url | https://www.dovepress.com/curcumin-loaded-redox-response--of-self-assembled-micelles-for-enhance-peer-reviewed-article-IJN |
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