Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors
<i>Bacillus cereus</i> is an opportunistic foodborne pathogen causing food intoxication and infectious diseases. Different toxins and pathogenic factors are responsible for diarrheal syndrome, like nonhemolytic enterotoxin Nhe, hemolytic enterotoxin Hbl, enterotoxin FM and cytotoxin K, w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/9/2667 |
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| author | Nalini Ramarao Seav-Ly Tran Marco Marin Jasmina Vidic |
| author_facet | Nalini Ramarao Seav-Ly Tran Marco Marin Jasmina Vidic |
| author_sort | Nalini Ramarao |
| collection | DOAJ |
| description | <i>Bacillus cereus</i> is an opportunistic foodborne pathogen causing food intoxication and infectious diseases. Different toxins and pathogenic factors are responsible for diarrheal syndrome, like nonhemolytic enterotoxin Nhe, hemolytic enterotoxin Hbl, enterotoxin FM and cytotoxin K, while emetic syndrome is caused by the depsipeptide cereulide toxin. The traditional method of <i>B. cereus</i> detection is based on the bacterial culturing onto selective agars and cells enumeration. In addition, molecular and chemical methods are proposed for toxin gene profiling, toxin quantification and strain screening for defined virulence factors. Finally, some advanced biosensors such as phage-based, cell-based, immunosensors and DNA biosensors have been elaborated to enable affordable, sensitive, user-friendly and rapid detection of specific <i>B. cereus</i> strains. This review intends to both illustrate the state of the <i>B. cereus</i> diagnostic field and to highlight additional research that is still at the development level. |
| first_indexed | 2024-03-10T19:59:39Z |
| format | Article |
| id | doaj.art-ab3905f74c944f12a542ac680fae49d9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T19:59:39Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ab3905f74c944f12a542ac680fae49d92023-11-19T23:41:44ZengMDPI AGSensors1424-82202020-05-01209266710.3390/s20092667Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic FactorsNalini Ramarao0Seav-Ly Tran1Marco Marin2Jasmina Vidic3INRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, France<i>Bacillus cereus</i> is an opportunistic foodborne pathogen causing food intoxication and infectious diseases. Different toxins and pathogenic factors are responsible for diarrheal syndrome, like nonhemolytic enterotoxin Nhe, hemolytic enterotoxin Hbl, enterotoxin FM and cytotoxin K, while emetic syndrome is caused by the depsipeptide cereulide toxin. The traditional method of <i>B. cereus</i> detection is based on the bacterial culturing onto selective agars and cells enumeration. In addition, molecular and chemical methods are proposed for toxin gene profiling, toxin quantification and strain screening for defined virulence factors. Finally, some advanced biosensors such as phage-based, cell-based, immunosensors and DNA biosensors have been elaborated to enable affordable, sensitive, user-friendly and rapid detection of specific <i>B. cereus</i> strains. This review intends to both illustrate the state of the <i>B. cereus</i> diagnostic field and to highlight additional research that is still at the development level.https://www.mdpi.com/1424-8220/20/9/2667<i>Bacillus cereus</i>bacterial detectionbiosensorstoxin detectionfood safetyhuman health |
| spellingShingle | Nalini Ramarao Seav-Ly Tran Marco Marin Jasmina Vidic Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors Sensors <i>Bacillus cereus</i> bacterial detection biosensors toxin detection food safety human health |
| title | Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors |
| title_full | Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors |
| title_fullStr | Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors |
| title_full_unstemmed | Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors |
| title_short | Advanced Methods for Detection of <i>Bacillus cereus</i> and Its Pathogenic Factors |
| title_sort | advanced methods for detection of i bacillus cereus i and its pathogenic factors |
| topic | <i>Bacillus cereus</i> bacterial detection biosensors toxin detection food safety human health |
| url | https://www.mdpi.com/1424-8220/20/9/2667 |
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